Neuronal Differentiation of Human Mesenchymal Stem Cells Using Exosomes Derived from Differentiating Neuronal Cells

• Published in: PloS one Vol. 10; no. 8; p. e0135111
• Main Authors: Takeda, Yuji S., Xu, Qiaobing
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 06.08.2015; Public Library of Science (PLoS)
• Subjects: Amino acids; Analysis; Animals; Biomedical engineering; Bone Marrow Cells - cytology; Bone Marrow Cells - drug effects; Bone Marrow Cells - metabolism; Cancer; Cell differentiation; Cell Differentiation - drug effects; Cell growth; Cell Line, Tumor; Cells (biology); Culture Media, Conditioned - chemistry; Culture Media, Conditioned - pharmacology; Differentiation; DNA microarrays; Exosomes; Exosomes - chemistry; Gene Expression Profiling; Gene Expression Regulation; Genetic aspects; Growth factors; Humans; Kinases; Melanoma; Melanoma, Experimental - chemistry; Mesenchymal stem cells; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - drug effects; Mesenchymal Stromal Cells - metabolism; Mesenchyme; Mice; MicroRNAs; MicroRNAs - genetics; MicroRNAs - metabolism; Microscopy; miRNA; Neural stem cells; Neurogenesis; Neurons; Neurons - cytology; Neurons - drug effects; Neurons - metabolism; Oligonucleotide Array Sequence Analysis; PC12 Cells; Pheochromocytoma cells; Physiological aspects; Primary Cell Culture; Progenitor cells; Proteins; Rats; Regeneration; Ribonucleic acid; RNA; Spinal cord injuries; Stem cells; Technology application; Tissue engineering; Transplants & implants; United States; United States > US

Exosomes deliver functional proteins and genetic materials to neighboring cells, and have potential applications for tissue regeneration. One possible mechanism of exosome-promoted tissue regeneration is through the delivery of microRNA (miRNA). In this study, we hypothesized that exosomes derived f...