Downregulation of carbonic anhydrase IX promotes Col10a1 expression in chondrocytes

• Published in: PloS one Vol. 8; no. 2; p. e56984
• Main Authors: Maruyama, Toshifumi, Miyamoto, Yoichi, Yamamoto, Gou, Yamada, Atsushi, Yoshimura, Kentaro, Suzawa, Tetsuo, Takami, Masamichi, Akiyama, Tomohito, Hoshino, Marie, Iwasa, Fuminori, Ikumi, Noriharu, Tachikawa, Tetsuhiko, Mishima, Kenji, Baba, Kazuyoshi, Kamijo, Ryutaro
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 18.02.2013; Public Library of Science (PLoS)
• Subjects: Animals; Basic Helix-Loop-Helix Transcription Factors - genetics; Biochemistry; Biology; Bromination; Cancer; Carbon dioxide; Carbonic anhydrase; Carbonic Anhydrase IX; Carbonic anhydrases; Carbonic Anhydrases - metabolism; Cartilage; Cell adhesion & migration; Cell Enlargement; Cell Hypoxia; Cell Proliferation; Chondrocytes; Chondrocytes - metabolism; Chondrocytes - pathology; Chondrogenesis; Collagen; Collagen Type X - genetics; Collagen Type X - metabolism; Cyclic AMP; Cyclic AMP-Dependent Protein Kinases - metabolism; Dental schools; Dentistry; Differentiation; Down-regulation; Embryos; Enzyme Activation; Enzyme inhibitors; Gene expression; Gene Expression Regulation; Genes; Growth Plate - metabolism; Hypoxia; Inhibitors; Kinases; Localization; Mice; Models, Biological; Pathology; Phosphodiesterase; Phosphorylation; Protein kinase A; Proteins; RNA; RNA Interference; Signal Transduction; siRNA; Studies; Transcription; Transcription factors; Tumors; Japan

Carbonic anhydrase (CA) IX is a transmembrane isozyme of CAs that catalyzes reversible hydration of CO(2). While it is known that CA IX is distributed in human embryonic chondrocytes, its role in chondrocyte differentiation has not been reported. In the present study, we found that Car9 mRNA and CA...