Probing Relevant Molecules in Modulating the Neurite Outgrowth of Hippocampal Neurons on Substrates of Different Stiffness

• Published in: PloS one Vol. 8; no. 12; p. e83394
• Main Authors: Chen, Wei-Hsin, Cheng, Sin-Jhong, Tzen, Jason T. C., Cheng, Chao-Min, Lin, Yi-Wen
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 30.12.2013; Public Library of Science (PLoS)
• Subjects: Animals; Axonogenesis; Biology; Cell adhesion & migration; Central nervous system; Curing agents; Dimethylpolysiloxane; Elongation; Engineering; Environmental effects; Extracellular matrix; Fibronectin; Fibronectins; Focal Adhesion Protein-Tyrosine Kinases - metabolism; Hippocampus; Hippocampus - cytology; Hippocampus - metabolism; Kinases; Learning; Lysine; Materials Science; Mechanotransduction; Medicine; Memory; Mice; Mitogen-Activated Protein Kinase 1 - metabolism; Mitogen-Activated Protein Kinase 3 - metabolism; Neurites - metabolism; Neurons; Poly-L-lysine; Polydimethylsiloxane; Primary Cell Culture; Protein Kinase C-alpha - metabolism; Pyramidal Cells - growth & development; Pyramidal Cells - metabolism; Rodents; Scaffolding; Signaling; Silicone resins; Stem cells; Stiffness; Substrates; Taiwan

Hippocampal neurons play a critical role in learning and memory; however, the effects of environmental mechanical forces on neurite extension and associated underlying mechanisms are largely unexplored, possibly due to difficulties in maintaining central nervous system neurons. Neuron adhesion, neur...