Purification of family B G protein-coupled receptors using nanodiscs: Application to human glucagon-like peptide-1 receptor

• Published in: PloS one Vol. 12; no. 6; p. e0179568
• Main Authors: Cai, Yingying, Liu, Yuting, Culhane, Kelly J, DeVree, Brian T, Yang, Yang, Sunahara, Roger K, Yan, Elsa C Y
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 13.06.2017; Public Library of Science (PLoS)
• Subjects: Activation analysis; Amino acids; Analysis; Apolipoproteins; Biochemistry; Biocompatibility; Biology and Life Sciences; Biomedical materials; Biophysics; Chemistry; Chromatography; Coupling (molecular); Denaturation; Density; Diabetes; Diabetes mellitus; Diabetes Mellitus, Type 2 - drug therapy; Diseases; Engineering and Technology; Environment; Exenatide; Functional anatomy; G protein-coupled receptors; G proteins; Glucagon; Glucagon-like peptide 1; Glucagon-Like Peptide 1 - metabolism; Glucagon-Like Peptide-1 Receptor - agonists; Glucagon-Like Peptide-1 Receptor - genetics; Glucagon-Like Peptide-1 Receptor - isolation & purification; Guanine nucleotide-binding protein; HEK293 Cells; Homeostasis; Humans; Hypoglycemic Agents - therapeutic use; Kinases; Labeling; Ligands; Lipid bilayers; Lipid Bilayers - chemistry; Lipid Bilayers - metabolism; Lipids; Lipoproteins, HDL - chemistry; Lipoproteins, HDL - metabolism; Metabolic disorders; Metabolism; Methods; Microscopy, Electron, Transmission; Mutagenesis; Nanostructure; Nanostructures - chemistry; Nanostructures - ultrastructure; Nanotechnology - methods; Osteoporosis; Particulates; Peptides; Peptides - metabolism; Peptides - therapeutic use; Physical Sciences; Protein Binding; Protein denaturation; Protein purification; Proteins; Purification; Receptors; Receptors, G-Protein-Coupled - agonists; Receptors, G-Protein-Coupled - genetics; Receptors, G-Protein-Coupled - isolation & purification; Reproducibility of Results; Rodents; Signal transduction; Solubilization; Spectroscopic analysis; Stabilization; Studies; Venoms - metabolism; Venoms - therapeutic use; United States > US; Connecticut; California

Family B G protein-coupled receptors (GPCRs) play vital roles in hormone-regulated homeostasis. They are drug targets for metabolic diseases, including type 2 diabetes and osteoporosis. Despite their importance, the signaling mechanisms for family B GPCRs at the molecular level remain largely unexpl...