An RT-PCR panel for rapid serotyping of dengue virus serotypes 1 to 4 in human serum and mosquito on a field-deployable PCR system

• Published in: PloS one Vol. 14; no. 3; p. e0214328
• Main Authors: Tsai, Jih-Jin, Liu, Wei-Liang, Lin, Ping-Chang, Huang, Bo-Yi, Tsai, Ching-Yi, Chou, Pin-Hsing, Lee, Fu-Chun, Ping, Chia-Fong, Lee, Pei-Yu Alison, Liu, Li-Teh, Chen, Chun-Hong
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 25.03.2019; Public Library of Science (PLoS)
• Subjects: Animals; Antigens; Aquatic insects; Biology and life sciences; Biotechnology; Chikungunya virus; Culicidae - virology; Dengue; Dengue - virology; Dengue fever; Dengue hemorrhagic fever; Dengue virus; Dengue Virus - classification; Dengue Virus - genetics; Disease control; Epidemics; Female; Fever; Health aspects; Health surveillance; Hospitals; Humans; Hygiene; Infection; Infections; Infectious diseases; Insect Vectors - virology; Laboratories; Medical research; Medical technology; Medicine; Medicine and Health Sciences; Methods; Mosquitoes; Outbreaks; Pathogenic microorganisms; Point-of-Care Systems; Polymerase chain reaction; Public health; Reagents; Research and Analysis Methods; Reverse Transcriptase Polymerase Chain Reaction; Reverse transcription; RNA, Viral - blood; Sensitivity analysis; Sensitivity and Specificity; Serotypes; Serotyping; Target detection; Underserved populations; Vector-borne diseases; Viral diseases; Viruses; Zika virus; Taiwan; Sri Lanka

Dengue fever, a mosquito-borne disease, is caused by dengue virus (DENV) which includes four major serotypes (DENV-1, -2, -3, and -4). Some serotypes cause more severe diseases than the other; severe dengue is associated with secondary infections by a different serotype. Timely serotyping can provid...