3D Cultures of prostate cancer cells cultured in a novel high-throughput culture platform are more resistant to chemotherapeutics compared to cells cultured in monolayer

• Published in: PloS one Vol. 9; no. 11; p. e111029
• Main Authors: Chambers, Karen F, Mosaad, Eman M O, Russell, Pamela J, Clements, Judith A, Doran, Michael R
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 07.11.2014; Public Library of Science (PLoS)
• Subjects: Antineoplastic Agents - pharmacology; Apoptosis; Apoptosis - drug effects; Biology; Biology and Life Sciences; Cancer; Cancer cells; Cell adhesion & migration; Cell Aggregation - drug effects; Cell culture; Cell Culture Techniques - methods; Cell Line, Tumor; Cell Proliferation - drug effects; Cell Survival - drug effects; Chemotherapy; Dimethylpolysiloxanes - chemistry; Drug development; Drug Resistance, Neoplasm - drug effects; Drug testing; Engineering and Technology; Extracellular matrix; Growth kinetics; Humans; In vivo methods and tests; Kinetics; Laboratories; Male; Medical research; Medical screening; Medicine and Health Sciences; Monolayers; Monomolecular films; Morphogenesis; Polarity; Prostate cancer; Prostatic Neoplasms - drug therapy; Prostatic Neoplasms - pathology; Research and Analysis Methods; Stem cells; Tissue Array Analysis; Tissues; Tumors; Queensland Australia; Australia

Despite monolayer cultures being widely used for cancer drug development and testing, 2D cultures tend to be hypersensitive to chemotherapy and are relatively poor predictors of whether a drug will provide clinical benefit. Whilst generally more complicated, three dimensional (3D) culture systems of...