Spinoculation Enhances HBV Infection in NTCP-Reconstituted Hepatocytes
Hepatitis B virus (HBV) infection and its sequelae remain a major public health burden, but both HBV basic research and the development of antiviral therapeutics have been hindered by the lack of an efficient in vitro infection system. Recently, sodium taurocholate cotransporting polypeptide (NTCP)...
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Published in | PloS one Vol. 10; no. 6; p. e0129889 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
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Public Library of Science
12.06.2015
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Abstract | Hepatitis B virus (HBV) infection and its sequelae remain a major public health burden, but both HBV basic research and the development of antiviral therapeutics have been hindered by the lack of an efficient in vitro infection system. Recently, sodium taurocholate cotransporting polypeptide (NTCP) has been identified as the HBV receptor. We herein report that we established a NTCP-complemented HepG2 cell line (HepG2-NTCP12) that supports HBV infection, albeit at a low infectivity level following the reported infection procedures. In our attempts to optimize the infection conditions, we found that the centrifugation of HepG2-NTCP12 cells during HBV inoculation (termed "spinoculation") significantly enhanced the virus infectivity. Moreover, the infection level gradually increased with accelerated speed of spinoculation up to 1,000g tested. However, the enhancement of HBV infection was not significantly dependent upon the duration of centrifugation. Furthermore, covalently closed circular (ccc) DNA was detected in infected cells under optimized infection condition by conventional Southern blot, suggesting a successful establishment of HBV infection after spinoculation. Finally, the parental HepG2 cells remained uninfected under HBV spinoculation, and HBV entry inhibitors targeting NTCP blocked HBV infection when cells were spinoculated, suggesting the authentic virus entry mechanism is unaltered under centrifugal inoculation. Our data suggest that spinoculation could serve as a standard protocol for enhancing the efficiency of HBV infection in vitro. |
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AbstractList | Hepatitis B virus (HBV) infection and its sequelae remain a major public health burden, but both HBV basic research and the development of antiviral therapeutics have been hindered by the lack of an efficient in vitro infection system. Recently, sodium taurocholate cotransporting polypeptide (NTCP) has been identified as the HBV receptor. We herein report that we established a NTCP-complemented HepG2 cell line (HepG2-NTCP12) that supports HBV infection, albeit at a low infectivity level following the reported infection procedures. In our attempts to optimize the infection conditions, we found that the centrifugation of HepG2-NTCP12 cells during HBV inoculation (termed "spinoculation") significantly enhanced the virus infectivity. Moreover, the infection level gradually increased with accelerated speed of spinoculation up to 1,000g tested. However, the enhancement of HBV infection was not significantly dependent upon the duration of centrifugation. Furthermore, covalently closed circular (ccc) DNA was detected in infected cells under optimized infection condition by conventional Southern blot, suggesting a successful establishment of HBV infection after spinoculation. Finally, the parental HepG2 cells remained uninfected under HBV spinoculation, and HBV entry inhibitors targeting NTCP blocked HBV infection when cells were spinoculated, suggesting the authentic virus entry mechanism is unaltered under centrifugal inoculation. Our data suggest that spinoculation could serve as a standard protocol for enhancing the efficiency of HBV infection in vitro. |
Audience | Academic |
Author | Yan, Ran Zhang, Yongmei Cuconati, Andrea Cai, Dawei Guo, Haitao Liu, Yuanjie |
AuthorAffiliation | 3 Baruch S. Blumberg Institute, Hepatitis B Foundation, 3805 Old Easton Rd, Doylestown, Pennsylvania, 18902, United States of America Academia Sinica, TAIWAN 1 Department of Microbiology and Immunology, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, Indiana, 46202, United States of America 2 Department of Infectious Diseases, Huashan Hospital, Fudan University, 12 Wulumuqi Zhong Rd, Shanghai, 200040, China |
AuthorAffiliation_xml | – name: 1 Department of Microbiology and Immunology, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, Indiana, 46202, United States of America – name: 3 Baruch S. Blumberg Institute, Hepatitis B Foundation, 3805 Old Easton Rd, Doylestown, Pennsylvania, 18902, United States of America – name: Academia Sinica, TAIWAN – name: 2 Department of Infectious Diseases, Huashan Hospital, Fudan University, 12 Wulumuqi Zhong Rd, Shanghai, 200040, China |
Author_xml | – sequence: 1 givenname: Ran surname: Yan fullname: Yan, Ran organization: Department of Microbiology and Immunology, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, Indiana, 46202, United States of America – sequence: 2 givenname: Yongmei surname: Zhang fullname: Zhang, Yongmei organization: Department of Infectious Diseases, Huashan Hospital, Fudan University, 12 Wulumuqi Zhong Rd, Shanghai, 200040, China – sequence: 3 givenname: Dawei surname: Cai fullname: Cai, Dawei organization: Department of Microbiology and Immunology, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, Indiana, 46202, United States of America – sequence: 4 givenname: Yuanjie surname: Liu fullname: Liu, Yuanjie organization: Department of Microbiology and Immunology, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, Indiana, 46202, United States of America – sequence: 5 givenname: Andrea surname: Cuconati fullname: Cuconati, Andrea organization: Baruch S. Blumberg Institute, Hepatitis B Foundation, 3805 Old Easton Rd, Doylestown, Pennsylvania, 18902, United States of America – sequence: 6 givenname: Haitao surname: Guo fullname: Guo, Haitao organization: Department of Microbiology and Immunology, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, Indiana, 46202, United States of America |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/26070202$$D View this record in MEDLINE/PubMed |
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Cites_doi | 10.1038/mtna.2014.68 10.1016/j.jviromet.2007.11.002 10.1002/hep.21896 10.1371/journal.ppat.1002255 10.1128/JVI.00561-08 10.1016/j.bbrc.2013.12.052 10.1073/pnas.92.17.7739 10.1111/j.1462-5822.2010.01557.x 10.1128/JVI.00873-12 10.1007/978-1-62703-484-5_13 10.1002/hep.26982 10.1016/j.cld.2007.08.002 10.1128/JVI.03478-13 10.1371/journal.pone.0110442 10.1371/journal.pone.0043270 10.1016/S0140-6736(14)60220-8 10.3181/00379727-111-27793 10.1128/JVI.05170-11 10.1002/jlb.53.2.208 10.1128/AAC.00473-12 10.1172/JCI117091 10.1053/j.gastro.2013.12.024 10.1038/nm0797-808 10.1038/nrmicro3098 10.1016/j.antiviral.2014.01.021 10.1128/IAI.21.1.281-285.1978 10.1128/JVI.01123-07 10.1128/MMBR.64.1.51-68.2000 10.1016/0166-0934(95)00035-S 10.7554/eLife.00049 10.1128/JVI.74.21.10074-10080.2000 10.1016/S0166-0934(01)00368-8 10.1128/JVI.01921-09 10.1016/j.jviromet.2005.04.008 10.1371/journal.ppat.1003494 10.1128/JVI.02721-06 10.1136/gutjnl-2013-304594 10.1128/JCM.27.7.1659-1660.1989 10.1053/j.gastro.2014.02.024 10.1016/S0168-8278(03)00120-X 10.1073/pnas.232137699 10.1016/0042-6822(76)90326-3 10.1016/j.jhep.2013.11.022 10.1128/JVI.00867-09 10.1128/AAC.00989-10 10.1021/mp300171p 10.1128/JVI.2.9.853-858.1968 |
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Copyright | COPYRIGHT 2015 Public Library of Science 2015 Yan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2015 Yan et al 2015 Yan et al |
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Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Current address: Oncore Biopharma, Inc., 3805 Old Easton Rd, Doylestown, Pennsylvania, 18902, United States of America Competing Interests: The authors have declared that no competing interests exist. Conceived and designed the experiments: RY AC HG. Performed the experiments: RY YZ DC. Analyzed the data: RY YZ DC YL AC HG. Wrote the paper: RY HG. |
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References | 24018384 - Nat Rev Microbiol. 2013 Oct;11(10):688-700 17981225 - Clin Liver Dis. 2007 Nov;11(4):685-706, vii 15908019 - J Virol Methods. 2005 Sep;128(1-2):104-12 24375637 - Hepatology. 2014 May;59(5):1726-37 4302185 - J Virol. 1968 Sep;2(9):853-8 17804499 - J Virol. 2007 Nov;81(22):12472-84 25337821 - PLoS One. 2014;9(10):e110442 23542357 - Gut. 2013 Aug;62(8):1093-5 24361467 - Gastroenterology. 2014 Apr;146(4):1070-83 17409153 - J Virol. 2007 Jun;81(12):6164-74 2549092 - J Clin Microbiol. 1989 Jul;27(7):1659-60 24342612 - Biochem Biophys Res Commun. 2014 Jan 17;443(3):808-13 10704474 - Microbiol Mol Biol Rev. 2000 Mar;64(1):51-68 8132774 - J Clin Invest. 1994 Mar;93(3):1326-31 7644487 - Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):7739-43 23150796 - Elife. 2012;1:e00049 11576644 - J Virol Methods. 2001 Nov;98(2):167-9 180669 - Virology. 1976 Jul 1;72(1):235-43 9212113 - Nat Med. 1997 Jul;3(7):808-11 24509240 - Antiviral Res. 2014 Apr;104:118-27 22740403 - J Virol. 2012 Sep;86(17):9443-53 21135183 - Antimicrob Agents Chemother. 2011 Feb;55(2):478-86 7680371 - J Leukoc Biol. 1993 Feb;53(2):208-12 21795326 - J Virol. 2011 Oct;85(19):9824-33 24576732 - Gastroenterology. 2014 Apr;146(4):902-5 24390325 - J Virol. 2014 Mar;88(6):3273-84 22897388 - Mol Pharm. 2012 Dec 3;9(12):3434-41 12763365 - J Hepatol. 2003 Jun;38(6):736-44 23853601 - PLoS Pathog. 2013;9(7):e1003494 8530565 - J Virol Methods. 1995 Aug;54(2-3):131-43 19625407 - J Virol. 2009 Oct;83(19):9652-62 21129143 - Cell Microbiol. 2011 Apr;13(4):602-19 21966269 - PLoS Pathog. 2011 Sep;7(9):e1002255 22912842 - PLoS One. 2012;7(8):e43270 13940995 - Proc Soc Exp Biol Med. 1962 Nov;111:364-7 23821267 - Methods Mol Biol. 2013;1030:151-61 19864387 - J Virol. 2010 Jan;84(1):387-96 25514649 - Mol Ther Nucleic Acids. 2014 Dec 16;3:e216 22644022 - Antimicrob Agents Chemother. 2012 Aug;56(8):4277-88 18178263 - J Virol Methods. 2008 Mar;148(1-2):161-5 18046710 - Hepatology. 2007 Dec;46(6):1759-68 18524834 - J Virol. 2008 Aug;82(16):7812-7 24954675 - Lancet. 2014 Dec 6;384(9959):2053-63 11024136 - J Virol. 2000 Nov;74(21):10074-80 24295872 - J Hepatol. 2014 Apr;60(4):723-31 213385 - Infect Immun. 1978 Jul;21(1):281-5 12432097 - Proc Natl Acad Sci U S A. 2002 Nov 26;99(24):15655-60 BA Bunnell (ref43) 1995; 92 R Mao (ref16) 2013; 9 IH Park (ref11) 2014; 104 BD Lindenbach (ref47) 2013; 11 H Guo (ref27) 2010; 84 C Seeger (ref1) 2000; 64 L Ye (ref41) 2008; 148 C Seeger (ref33) 2013; 62 H Guo (ref17) 2011; 55 D Cai (ref18) 2012; 56 C Trepo (ref3) 2014; 384 S Tong (ref32) 2014; 146 M Iwamoto (ref10) 2014; 443 RB Tenser (ref37) 1978; 21 D Cai (ref25) 2013; 1030 DR Sundin (ref38) 1989; 27 W Gao (ref26) 2007; 81 TM Block (ref2) 2007; 11 H Yan (ref7) 2012; 1 JB Hudson (ref35) 1976; 72 K Watashi (ref31) 2014; 59 P Gripon (ref6) 2002; 99 Y Ni (ref9) 2014; 146 H Guo (ref28) 2012; 7 N Kotani (ref24) 2012; 9 PM Scanlan (ref44) 2005; 128 B Hagenbuch (ref21) 1994; 93 WZ Ho (ref36) 1993; 53 C Seeger (ref8) 2014; 3 J Guo (ref14) 2011; 85 U O'Doherty (ref15) 2000; 74 AB Bahnson (ref42) 1995; 54 BL Padgett (ref12) 1962; 111 X Ning (ref23) 2011; 7 S Nkongolo (ref30) 2014; 60 N Chai (ref34) 2008; 82 A Bardens (ref22) 2011; 13 JE Osborn (ref13) 1968; 2 Y Zhang (ref19) 2014; 9 S Asabe (ref4) 2009; 83 H Guo (ref20) 2007; 81 H Yan (ref29) 2014; 88 HC Huang (ref46) 2012; 86 C Nyberg-Hoffman (ref39) 1997; 3 SA Reading (ref40) 2001; 98 H Schulze-Bergkamen (ref5) 2003; 38 A Schulze (ref45) 2007; 46 |
References_xml | – volume: 3 start-page: e216 year: 2014 ident: ref8 article-title: Targeting Hepatitis B Virus With CRISPR/Cas9 publication-title: Molecular therapy Nucleic acids doi: 10.1038/mtna.2014.68 contributor: fullname: C Seeger – volume: 148 start-page: 161 issue: 1–2 year: 2008 ident: ref41 article-title: Centrifugal enhancement of hepatitis C virus infection of human hepatocytes publication-title: Journal of virological methods doi: 10.1016/j.jviromet.2007.11.002 contributor: fullname: L Ye – volume: 46 start-page: 1759 issue: 6 year: 2007 ident: ref45 article-title: Hepatitis B virus infection initiates with a large surface protein-dependent binding to heparan sulfate proteoglycans publication-title: Hepatology doi: 10.1002/hep.21896 contributor: fullname: A Schulze – volume: 7 start-page: e1002255 issue: 9 year: 2011 ident: ref23 article-title: Secretion of genome-free hepatitis B virus—single strand blocking model for virion morphogenesis of para-retrovirus publication-title: PLoS pathogens doi: 10.1371/journal.ppat.1002255 contributor: fullname: X Ning – volume: 82 start-page: 7812 issue: 16 year: 2008 ident: ref34 article-title: Properties of subviral particles of hepatitis B virus publication-title: Journal of virology doi: 10.1128/JVI.00561-08 contributor: fullname: N Chai – volume: 443 start-page: 808 issue: 3 year: 2014 ident: ref10 article-title: Evaluation and identification of hepatitis B virus entry inhibitors using HepG2 cells overexpressing a membrane transporter NTCP publication-title: Biochemical and biophysical research communications doi: 10.1016/j.bbrc.2013.12.052 contributor: fullname: M Iwamoto – volume: 92 start-page: 7739 issue: 17 year: 1995 ident: ref43 article-title: High-efficiency retroviral-mediated gene transfer into human and nonhuman primate peripheral blood lymphocytes publication-title: Proceedings of the National Academy of Sciences of the United States of America doi: 10.1073/pnas.92.17.7739 contributor: fullname: BA Bunnell – volume: 13 start-page: 602 issue: 4 year: 2011 ident: ref22 article-title: Alix regulates egress of hepatitis B virus naked capsid particles in an ESCRT-independent manner publication-title: Cellular microbiology doi: 10.1111/j.1462-5822.2010.01557.x contributor: fullname: A Bardens – volume: 86 start-page: 9443 issue: 17 year: 2012 ident: ref46 article-title: Entry of hepatitis B virus into immortalized human primary hepatocytes by clathrin-dependent endocytosis publication-title: Journal of virology doi: 10.1128/JVI.00873-12 contributor: fullname: HC Huang – volume: 1030 start-page: 151 year: 2013 ident: ref25 article-title: A southern blot assay for detection of hepatitis B virus covalently closed circular DNA from cell cultures publication-title: Methods in molecular biology doi: 10.1007/978-1-62703-484-5_13 contributor: fullname: D Cai – volume: 59 start-page: 1726 issue: 5 year: 2014 ident: ref31 article-title: Cyclosporin A and its analogs inhibit hepatitis B virus entry into cultured hepatocytes through targeting a membrane transporter, sodium taurocholate cotransporting polypeptide (NTCP) publication-title: Hepatology doi: 10.1002/hep.26982 contributor: fullname: K Watashi – volume: 11 start-page: 685 issue: 4 year: 2007 ident: ref2 article-title: Molecular virology of hepatitis B virus for clinicians publication-title: Clinics in liver disease doi: 10.1016/j.cld.2007.08.002 contributor: fullname: TM Block – volume: 88 start-page: 3273 issue: 6 year: 2014 ident: ref29 article-title: Viral entry of hepatitis B and D viruses and bile salts transportation share common molecular determinants on sodium taurocholate cotransporting polypeptide publication-title: Journal of virology doi: 10.1128/JVI.03478-13 contributor: fullname: H Yan – volume: 9 start-page: e110442 issue: 10 year: 2014 ident: ref19 article-title: Transcription of hepatitis B virus covalently closed circular DNA is regulated by CpG methylation during chronic infection publication-title: PloS one doi: 10.1371/journal.pone.0110442 contributor: fullname: Y Zhang – volume: 7 start-page: e43270 issue: 8 year: 2012 ident: ref28 article-title: Characterization of the host factors required for hepadnavirus covalently closed circular (ccc) DNA formation publication-title: PloS one doi: 10.1371/journal.pone.0043270 contributor: fullname: H Guo – volume: 384 start-page: 2053 issue: 9959 year: 2014 ident: ref3 article-title: Hepatitis B virus infection publication-title: Lancet doi: 10.1016/S0140-6736(14)60220-8 contributor: fullname: C Trepo – volume: 111 start-page: 364 year: 1962 ident: ref12 article-title: Use of centrifugal force to promote adsorption of myxoma virus to cell monolayers publication-title: Proceedings of the Society for Experimental Biology and Medicine Society for Experimental Biology and Medicine doi: 10.3181/00379727-111-27793 contributor: fullname: BL Padgett – volume: 85 start-page: 9824 issue: 19 year: 2011 ident: ref14 article-title: Spinoculation triggers dynamic actin and cofilin activity that facilitates HIV-1 infection of transformed and resting CD4 T cells publication-title: Journal of virology doi: 10.1128/JVI.05170-11 contributor: fullname: J Guo – volume: 53 start-page: 208 issue: 2 year: 1993 ident: ref36 article-title: Centrifugal enhancement of human immunodeficiency virus type 1 infection and human cytomegalovirus gene expression in human primary monocyte/macrophages in vitro publication-title: Journal of leukocyte biology doi: 10.1002/jlb.53.2.208 contributor: fullname: WZ Ho – volume: 56 start-page: 4277 issue: 8 year: 2012 ident: ref18 article-title: Identification of disubstituted sulfonamide compounds as specific inhibitors of hepatitis B virus covalently closed circular DNA formation publication-title: Antimicrobial agents and chemotherapy doi: 10.1128/AAC.00473-12 contributor: fullname: D Cai – volume: 93 start-page: 1326 issue: 3 year: 1994 ident: ref21 article-title: Molecular cloning, chromosomal localization, and functional characterization of a human liver Na+/bile acid cotransporter publication-title: The Journal of clinical investigation doi: 10.1172/JCI117091 contributor: fullname: B Hagenbuch – volume: 146 start-page: 1070 issue: 4 year: 2014 ident: ref9 article-title: Hepatitis B and D viruses exploit sodium taurocholate co-transporting polypeptide for species-specific entry into hepatocytes publication-title: Gastroenterology doi: 10.1053/j.gastro.2013.12.024 contributor: fullname: Y Ni – volume: 3 start-page: 808 issue: 7 year: 1997 ident: ref39 article-title: Sensitivity and reproducibility in adenoviral infectious titer determination publication-title: Nature medicine doi: 10.1038/nm0797-808 contributor: fullname: C Nyberg-Hoffman – volume: 11 start-page: 688 issue: 10 year: 2013 ident: ref47 article-title: The ins and outs of hepatitis C virus entry and assembly publication-title: Nature reviews Microbiology doi: 10.1038/nrmicro3098 contributor: fullname: BD Lindenbach – volume: 104 start-page: 118 year: 2014 ident: ref11 article-title: Inhibition of hepatitis B virus replication by ligand-mediated activation of RNase L publication-title: Antiviral research doi: 10.1016/j.antiviral.2014.01.021 contributor: fullname: IH Park – volume: 21 start-page: 281 issue: 1 year: 1978 ident: ref37 article-title: Ultracentrifugal inoculation of herpes simplex virus publication-title: Infection and immunity doi: 10.1128/IAI.21.1.281-285.1978 contributor: fullname: RB Tenser – volume: 81 start-page: 12472 issue: 22 year: 2007 ident: ref20 article-title: Characterization of the intracellular deproteinized relaxed circular DNA of hepatitis B virus: an intermediate of covalently closed circular DNA formation publication-title: Journal of virology doi: 10.1128/JVI.01123-07 contributor: fullname: H Guo – volume: 64 start-page: 51 issue: 1 year: 2000 ident: ref1 article-title: Hepatitis B virus biology publication-title: Microbiology and molecular biology reviews: MMBR doi: 10.1128/MMBR.64.1.51-68.2000 contributor: fullname: C Seeger – volume: 54 start-page: 131 issue: 2–3 year: 1995 ident: ref42 article-title: Centrifugal enhancement of retroviral mediated gene transfer publication-title: Journal of virological methods doi: 10.1016/0166-0934(95)00035-S contributor: fullname: AB Bahnson – volume: 1 start-page: e00049 year: 2012 ident: ref7 article-title: Sodium taurocholate cotransporting polypeptide is a functional receptor for human hepatitis B and D virus publication-title: eLife doi: 10.7554/eLife.00049 contributor: fullname: H Yan – volume: 74 start-page: 10074 issue: 21 year: 2000 ident: ref15 article-title: Human immunodeficiency virus type 1 spinoculation enhances infection through virus binding publication-title: Journal of virology doi: 10.1128/JVI.74.21.10074-10080.2000 contributor: fullname: U O'Doherty – volume: 98 start-page: 167 issue: 2 year: 2001 ident: ref40 article-title: Increasing the efficiency of virus infectivity assays: small inoculum volumes are as effective as centrifugal enhancement publication-title: Journal of virological methods doi: 10.1016/S0166-0934(01)00368-8 contributor: fullname: SA Reading – volume: 84 start-page: 387 issue: 1 year: 2010 ident: ref27 article-title: Production and function of the cytoplasmic deproteinized relaxed circular DNA of hepadnaviruses publication-title: Journal of virology doi: 10.1128/JVI.01921-09 contributor: fullname: H Guo – volume: 128 start-page: 104 issue: 1–2 year: 2005 ident: ref44 article-title: Spinoculation of heparan sulfate deficient cells enhances HSV-1 entry, but does not abolish the need for essential glycoproteins in viral fusion publication-title: Journal of virological methods doi: 10.1016/j.jviromet.2005.04.008 contributor: fullname: PM Scanlan – volume: 9 start-page: e1003494 issue: 7 year: 2013 ident: ref16 article-title: Inhibition of hepatitis B virus replication by the host zinc finger antiviral protein publication-title: PLoS pathogens doi: 10.1371/journal.ppat.1003494 contributor: fullname: R Mao – volume: 81 start-page: 6164 issue: 12 year: 2007 ident: ref26 article-title: Formation of hepatitis B virus covalently closed circular DNA: removal of genome-linked protein publication-title: Journal of virology doi: 10.1128/JVI.02721-06 contributor: fullname: W Gao – volume: 62 start-page: 1093 issue: 8 year: 2013 ident: ref33 article-title: Sodium-dependent taurocholic cotransporting polypeptide: a candidate receptor for human hepatitis B virus publication-title: Gut doi: 10.1136/gutjnl-2013-304594 contributor: fullname: C Seeger – volume: 27 start-page: 1659 issue: 7 year: 1989 ident: ref38 article-title: Enhanced infectivity of bluetongue virus in cell culture by centrifugation publication-title: Journal of clinical microbiology doi: 10.1128/JCM.27.7.1659-1660.1989 contributor: fullname: DR Sundin – volume: 146 start-page: 902 issue: 4 year: 2014 ident: ref32 article-title: Identification of NTCP as an HBV receptor: the beginning of the end or the end of the beginning? publication-title: Gastroenterology doi: 10.1053/j.gastro.2014.02.024 contributor: fullname: S Tong – volume: 38 start-page: 736 issue: 6 year: 2003 ident: ref5 article-title: Primary human hepatocytes—a valuable tool for investigation of apoptosis and hepatitis B virus infection publication-title: Journal of hepatology doi: 10.1016/S0168-8278(03)00120-X contributor: fullname: H Schulze-Bergkamen – volume: 99 start-page: 15655 issue: 24 year: 2002 ident: ref6 article-title: Infection of a human hepatoma cell line by hepatitis B virus publication-title: Proceedings of the National Academy of Sciences of the United States of America doi: 10.1073/pnas.232137699 contributor: fullname: P Gripon – volume: 72 start-page: 235 issue: 1 year: 1976 ident: ref35 article-title: Cytomegalovirus infectivity: analysis of the phenomenon of centrifugal enhancement of infectivity publication-title: Virology doi: 10.1016/0042-6822(76)90326-3 contributor: fullname: JB Hudson – volume: 60 start-page: 723 issue: 4 year: 2014 ident: ref30 article-title: Cyclosporin A inhibits hepatitis B and hepatitis D virus entry by cyclophilin-independent interference with the NTCP receptor publication-title: Journal of hepatology doi: 10.1016/j.jhep.2013.11.022 contributor: fullname: S Nkongolo – volume: 83 start-page: 9652 issue: 19 year: 2009 ident: ref4 article-title: The size of the viral inoculum contributes to the outcome of hepatitis B virus infection publication-title: Journal of virology doi: 10.1128/JVI.00867-09 contributor: fullname: S Asabe – volume: 55 start-page: 478 issue: 2 year: 2011 ident: ref17 article-title: Alkylated porphyrins have broad antiviral activity against hepadnaviruses, flaviviruses, filoviruses, and arenaviruses publication-title: Antimicrobial agents and chemotherapy doi: 10.1128/AAC.00989-10 contributor: fullname: H Guo – volume: 9 start-page: 3434 issue: 12 year: 2012 ident: ref24 article-title: Expression and transport function of drug uptake transporters in differentiated HepaRG cells publication-title: Molecular pharmaceutics doi: 10.1021/mp300171p contributor: fullname: N Kotani – volume: 2 start-page: 853 issue: 9 year: 1968 ident: ref13 article-title: Enhancement of infectivity of murine cytomegalovirus in vitro by centrifugal inoculation publication-title: Journal of virology doi: 10.1128/JVI.2.9.853-858.1968 contributor: fullname: JE Osborn |
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SubjectTerms | Antiviral agents Cell culture Centrifugation Circular DNA Complications Cytomegalovirus Deoxyribonucleic acid DNA Drug development Gene Expression Genome, Viral Genomes Health aspects Hep G2 Cells Hepatitis Hepatitis B Hepatitis B virus - physiology Hepatocytes Hepatocytes - metabolism Hepatocytes - virology Hepatology Humans Immunology In Vitro Techniques Infection Infections Infectivity Inoculation Medicine Organic Anion Transporters, Sodium-Dependent - genetics Plasmids Polypeptides Public health Sodium Symporters - genetics Virion Virology Virus Internalization Viruses |
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Title | Spinoculation Enhances HBV Infection in NTCP-Reconstituted Hepatocytes |
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