Direct and dynamic detection of HIV-1 in living cells

• Published in: PloS one Vol. 7; no. 11; p. e50026
• Main Authors: Helma, Jonas, Schmidthals, Katrin, Lux, Vanda, Nüske, Stefan, Scholz, Armin M, Kräusslich, Hans-Georg, Rothbauer, Ulrich, Leonhardt, Heinrich
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 28.11.2012; Public Library of Science (PLoS)
• Subjects: Amino Acid Sequence; Antibodies; Antibody Affinity - immunology; Antigens; Biology; Biosensors; Capsid protein; Cell Membrane - metabolism; Cells (biology); Chromatography; Detection equipment; Development and progression; Diagnostic systems; Fluorescence; gag Gene Products, Human Immunodeficiency Virus - immunology; gag Gene Products, Human Immunodeficiency Virus - metabolism; Gag protein; Genetic modification; Genetically modified organisms; Health aspects; HeLa Cells; HIV; HIV Core Protein p24 - chemistry; HIV Core Protein p24 - immunology; HIV Core Protein p24 - metabolism; HIV tests; HIV-1 - immunology; HIV-1 - isolation & purification; HIV-1 - metabolism; Human immunodeficiency virus; Humans; Image analysis; Image processing; Immunoglobulins; Infection; Infectious diseases; Kinetics; Light microscopy; Medicine; Microscopy; Microscopy, Confocal; Molecular Imaging; Molecular Sequence Data; Morphogenesis; Nanobodies; Protein binding; Protein Binding - immunology; Protein Transport; Proteins; Recovery (Medical); Single-Domain Antibodies - immunology; Single-Domain Antibodies - metabolism; Time-Lapse Imaging; Veterinary medicine; Viral proteins; Virions; Virology; Virus Assembly; Viruses; Germany

In basic and applied HIV research, reliable detection of viral components is crucial to monitor progression of infection. While it is routine to detect structural viral proteins in vitro for diagnostic purposes, it previously remained impossible to directly and dynamically visualize HIV in living ce...