Characterizing the Mechanism of Action of Double-Stranded RNA Activity against Western Corn Rootworm (Diabrotica virgifera virgifera LeConte)

• Published in: PloS one Vol. 7; no. 10; p. e47534
• Main Authors: Bolognesi, Renata, Ramaseshadri, Parthasarathy, Anderson, Jerry, Bachman, Pamela, Clinton, William, Flannagan, Ronald, Ilagan, Oliver, Lawrence, Christina, Levine, Steven, Moar, William, Mueller, Geoffrey, Tan, Jianguo, Uffman, Joshua, Wiggins, Elizabeth, Heck, Gregory, Segers, Gerrit
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 11.10.2012; Public Library of Science (PLoS)
• Subjects: Agriculture; Analysis of Variance; Animals; Artificial diets; Base Sequence; Bioassays; Biological activity; Biological Assay; Biology; Biopesticides; Biotechnology; Blotting, Western; Chemical industry; Coleoptera; Coleoptera - drug effects; Coleoptera - genetics; Corn; Diabrotica virgifera virgifera; Diet; Diseases and pests; Double-stranded RNA; Drosophila; Endosomal Sorting Complexes Required for Transport - genetics; Endosomal Sorting Complexes Required for Transport - metabolism; Enzyme-Linked Immunosorbent Assay; Gastrointestinal Tract - metabolism; Gene expression; Genetic aspects; Helicoverpa armigera; Ingestion; Insect Control - methods; Insects; Larva - drug effects; Larvae; Lethal Dose 50; Machinery and equipment; Midgut; Molecular Sequence Data; Mortality; mRNA; Organisms; Physiological aspects; Plant tissues; Protein transport; Proteins; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; Ribonucleic acid; RNA; RNA Interference; RNA, Double-Stranded - genetics; RNA, Double-Stranded - toxicity; RNA-mediated interference; siRNA; Spodoptera exigua; Studies; Transgenic plants; Trends; Worms (Animals); Zea mays; United States > US; Missouri

RNA interference (RNAi) has previously been shown to be effective in western corn rootworm (WCR, Diabrotica virgifera virgifera LeConte) larvae via oral delivery of synthetic double-stranded RNA (dsRNA) in an artificial diet bioassay, as well as by ingestion of transgenic corn plant tissues engineer...