A DNA metabarcoding approach for recovering plankton communities from archived samples fixed in formalin

• Published in: PloS one Vol. 16; no. 2; p. e0245936
• Main Authors: Shiozaki, Takuhei, Itoh, Fumihiro, Hirose, Yuu, Onodera, Jonaotaro, Kuwata, Akira, Harada, Naomi
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 17.02.2021; Public Library of Science (PLoS)
• Subjects: Biological diversity; Biological specimens; Biology and Life Sciences; Deoxyribonucleic acid; DNA; DNA barcoding; Earth; Earth science; Ecology and Environmental Sciences; Editing; Environmental science; Fisheries; Fisheries research; Fishery resources; Formaldehyde; Funding; Gene amplification; Genetic aspects; Laboratories; Marine technology; Medical research; Methodology; Methods; Microscopy; Museums; Natural history; Oceanographic research; Plankton; Plankton research; Research and analysis methods; Reviews; Science and technology; Seawater; Sediments; Technology; Visualization; Japan

Plankton samples have been routinely collected and preserved in formalin in many laboratories and museums for more than 100 years. Recently, attention has turned to use DNA information from formalin-fixed samples to examine changes in plankton diversity over time. However, no molecular ecological st...