Phenotypic characterization of prostate cancer LNCaP cells cultured within a bioengineered microenvironment

• Published in: PloS one Vol. 7; no. 9; p. e40217
• Main Authors: Sieh, Shirly, Taubenberger, Anna V, Rizzi, Simone C, Sadowski, Martin, Lehman, Melanie L, Rockstroh, Anja, An, Jiyuan, Clements, Judith A, Nelson, Colleen C, Hutmacher, Dietmar W
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 05.09.2012; Public Library of Science (PLoS)
• Subjects: Active Transport, Cell Nucleus; Amino Acid Motifs; Androgen receptors; Androgens; Apoptosis; Arginine; Aspartic acid; Binding sites; Bioengineering; Biology; Biomimetics; Biophysics - methods; Cancer; Cell culture; Cell Culture Techniques; Cell Differentiation; Cell growth; Cell Line, Tumor; Chemistry; Collagen; Collagen - chemistry; Cores; Degradability; Differentiation; Diffusion; DNA microarrays; Drug Combinations; Engineering; Evolution & development; Extracellular matrix; Extracellular Matrix - metabolism; Gene expression; Glycine; Growth kinetics; Hospitals; Humans; Hydrogel, Polyethylene Glycol Dimethacrylate; Hydrogels; Hydrogels - chemistry; Hypoxia; Kinases; Kinetics; Laminin - chemistry; Ligands; Male; Materials Science; Matrix metalloproteinase; Mechanical properties; Medical prognosis; Medical research; Medicine; Mens health; Metalloproteinase; Morphogenesis; Morphology; Nuclear transport; Phenotype; Polyethylene glycol; Prostate cancer; Prostatic Neoplasms - pathology; Protein binding; Proteins; Proteoglycans - chemistry; Proteolysis; Receptors, Androgen - metabolism; Stiffness; Stimulation; Stress, Mechanical; Studies; Time Factors; Translocation; Canada; Queensland Australia; Vancouver British Columbia Canada; Australia

Biophysical and biochemical properties of the microenvironment regulate cellular responses such as growth, differentiation, morphogenesis and migration in normal and cancer cells. Since two-dimensional (2D) cultures lack the essential characteristics of the native cellular microenvironment, three-di...