Phenotypic characterization of prostate cancer LNCaP cells cultured within a bioengineered microenvironment
Biophysical and biochemical properties of the microenvironment regulate cellular responses such as growth, differentiation, morphogenesis and migration in normal and cancer cells. Since two-dimensional (2D) cultures lack the essential characteristics of the native cellular microenvironment, three-di...
Saved in:
Published in | PloS one Vol. 7; no. 9; p. e40217 |
---|---|
Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Public Library of Science
05.09.2012
Public Library of Science (PLoS) |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Biophysical and biochemical properties of the microenvironment regulate cellular responses such as growth, differentiation, morphogenesis and migration in normal and cancer cells. Since two-dimensional (2D) cultures lack the essential characteristics of the native cellular microenvironment, three-dimensional (3D) cultures have been developed to better mimic the natural extracellular matrix. To date, 3D culture systems have relied mostly on collagen and Matrigel™ hydrogels, allowing only limited control over matrix stiffness, proteolytic degradability, and ligand density. In contrast, bioengineered hydrogels allow us to independently tune and systematically investigate the influence of these parameters on cell growth and differentiation. In this study, polyethylene glycol (PEG) hydrogels, functionalized with the Arginine-glycine-aspartic acid (RGD) motifs, common cell-binding motifs in extracellular matrix proteins, and matrix metalloproteinase (MMP) cleavage sites, were characterized regarding their stiffness, diffusive properties, and ability to support growth of androgen-dependent LNCaP prostate cancer cells. We found that the mechanical properties modulated the growth kinetics of LNCaP cells in the PEG hydrogel. At culture periods of 28 days, LNCaP cells underwent morphogenic changes, forming tumor-like structures in 3D culture, with hypoxic and apoptotic cores. We further compared protein and gene expression levels between 3D and 2D cultures upon stimulation with the synthetic androgen R1881. Interestingly, the kinetics of R1881 stimulated androgen receptor (AR) nuclear translocation differed between 2D and 3D cultures when observed by immunofluorescent staining. Furthermore, microarray studies revealed that changes in expression levels of androgen responsive genes upon R1881 treatment differed greatly between 2D and 3D cultures. Taken together, culturing LNCaP cells in the tunable PEG hydrogels reveals differences in the cellular responses to androgen stimulation between the 2D and 3D environments. Therefore, we suggest that the presented 3D culture system represents a powerful tool for high throughput prostate cancer drug testing that recapitulates tumor microenvironment. |
---|---|
AbstractList | Biophysical and biochemical properties of the microenvironment regulate cellular responses such as growth, differentiation, morphogenesis and migration in normal and cancer cells. Since two-dimensional (2D) cultures lack the essential characteristics of the native cellular microenvironment, three-dimensional (3D) cultures have been developed to better mimic the natural extracellular matrix. To date, 3D culture systems have relied mostly on collagen and Matrigel[TM] hydrogels, allowing only limited control over matrix stiffness, proteolytic degradability, and ligand density. In contrast, bioengineered hydrogels allow us to independently tune and systematically investigate the influence of these parameters on cell growth and differentiation. In this study, polyethylene glycol (PEG) hydrogels, functionalized with the Arginine-glycine-aspartic acid (RGD) motifs, common cell-binding motifs in extracellular matrix proteins, and matrix metalloproteinase (MMP) cleavage sites, were characterized regarding their stiffness, diffusive properties, and ability to support growth of androgen-dependent LNCaP prostate cancer cells. We found that the mechanical properties modulated the growth kinetics of LNCaP cells in the PEG hydrogel. At culture periods of 28 days, LNCaP cells underwent morphogenic changes, forming tumor-like structures in 3D culture, with hypoxic and apoptotic cores. We further compared protein and gene expression levels between 3D and 2D cultures upon stimulation with the synthetic androgen R1881. Interestingly, the kinetics of R1881 stimulated androgen receptor (AR) nuclear translocation differed between 2D and 3D cultures when observed by immunofluorescent staining. Furthermore, microarray studies revealed that changes in expression levels of androgen responsive genes upon R1881 treatment differed greatly between 2D and 3D cultures. Taken together, culturing LNCaP cells in the tunable PEG hydrogels reveals differences in the cellular responses to androgen stimulation between the 2D and 3D environments. Therefore, we suggest that the presented 3D culture system represents a powerful tool for high throughput prostate cancer drug testing that recapitulates tumor microenvironment. Biophysical and biochemical properties of the microenvironment regulate cellular responses such as growth, differentiation, morphogenesis and migration in normal and cancer cells. Since two-dimensional (2D) cultures lack the essential characteristics of the native cellular microenvironment, three-dimensional (3D) cultures have been developed to better mimic the natural extracellular matrix. To date, 3D culture systems have relied mostly on collagen and Matrigel™ hydrogels, allowing only limited control over matrix stiffness, proteolytic degradability, and ligand density. In contrast, bioengineered hydrogels allow us to independently tune and systematically investigate the influence of these parameters on cell growth and differentiation. In this study, polyethylene glycol (PEG) hydrogels, functionalized with the Arginine-glycine-aspartic acid (RGD) motifs, common cell-binding motifs in extracellular matrix proteins, and matrix metalloproteinase (MMP) cleavage sites, were characterized regarding their stiffness, diffusive properties, and ability to support growth of androgen-dependent LNCaP prostate cancer cells. We found that the mechanical properties modulated the growth kinetics of LNCaP cells in the PEG hydrogel. At culture periods of 28 days, LNCaP cells underwent morphogenic changes, forming tumor-like structures in 3D culture, with hypoxic and apoptotic cores. We further compared protein and gene expression levels between 3D and 2D cultures upon stimulation with the synthetic androgen R1881. Interestingly, the kinetics of R1881 stimulated androgen receptor (AR) nuclear translocation differed between 2D and 3D cultures when observed by immunofluorescent staining. Furthermore, microarray studies revealed that changes in expression levels of androgen responsive genes upon R1881 treatment differed greatly between 2D and 3D cultures. Taken together, culturing LNCaP cells in the tunable PEG hydrogels reveals differences in the cellular responses to androgen stimulation between the 2D and 3D environments. Therefore, we suggest that the presented 3D culture system represents a powerful tool for high throughput prostate cancer drug testing that recapitulates tumor microenvironment. |
Audience | Academic |
Author | Taubenberger, Anna V Rockstroh, Anja Hutmacher, Dietmar W Sadowski, Martin An, Jiyuan Clements, Judith A Rizzi, Simone C Lehman, Melanie L Sieh, Shirly Nelson, Colleen C |
AuthorAffiliation | University of North Carolina at Chapel Hill, United States of America 1 Regenerative Medicine and Cancer Program, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia 3 Department of Urologic Sciences, Vancouver Prostate Centre, University of British Columbia, Vancouver, British Columbia, Canada 2 Australian Prostate Cancer Research Centre-Queensland, Queensland University of Technology and Princess Alexandra Hospital, Woolloongabba, Queensland, Australia |
AuthorAffiliation_xml | – name: 2 Australian Prostate Cancer Research Centre-Queensland, Queensland University of Technology and Princess Alexandra Hospital, Woolloongabba, Queensland, Australia – name: University of North Carolina at Chapel Hill, United States of America – name: 3 Department of Urologic Sciences, Vancouver Prostate Centre, University of British Columbia, Vancouver, British Columbia, Canada – name: 1 Regenerative Medicine and Cancer Program, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia |
Author_xml | – sequence: 1 givenname: Shirly surname: Sieh fullname: Sieh, Shirly organization: Regenerative Medicine and Cancer Program, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia – sequence: 2 givenname: Anna V surname: Taubenberger fullname: Taubenberger, Anna V – sequence: 3 givenname: Simone C surname: Rizzi fullname: Rizzi, Simone C – sequence: 4 givenname: Martin surname: Sadowski fullname: Sadowski, Martin – sequence: 5 givenname: Melanie L surname: Lehman fullname: Lehman, Melanie L – sequence: 6 givenname: Anja surname: Rockstroh fullname: Rockstroh, Anja – sequence: 7 givenname: Jiyuan surname: An fullname: An, Jiyuan – sequence: 8 givenname: Judith A surname: Clements fullname: Clements, Judith A – sequence: 9 givenname: Colleen C surname: Nelson fullname: Nelson, Colleen C – sequence: 10 givenname: Dietmar W surname: Hutmacher fullname: Hutmacher, Dietmar W |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/22957009$$D View this record in MEDLINE/PubMed |
BookMark | eNqNk1tv0zAUxyM0xC7wDRBEQkLw0OJLHCcvSFPFpVLFJm6vluOcNC6p3dnOYHx6HJpNDdoDykPik9_5-1xPkyNjDSTJU4zmmHL8ZmN7Z2Q330XzHKEMEcwfJCe4pGSWE0SPDr6Pk1PvNwgxWuT5o-SYkJJxhMqT5MdlC8aGm51WqWqlkyqA079l0Naktkl3zvogA6RKGgUuXX1ayMtUQdf5VPVd6B3U6U8dWm1SmVbagllrAzCYt1q5eL7WzpotmPA4edjIzsOT8X2WfHv_7uvi42x18WG5OF_NFGdFmOUqB4kJQxxqhogCRBUtmkrWjKgGatkQYKSqYsKSZ4jmGHFUNmXJFFZ1mdGz5Pled9dZL8Y6eYEpRUWRMVRGYrknais3Yuf0VrobYaUWfw3WrYV0QasOBJWAVYxA8TzPWFFUBYYmb0hB6qLMaxa13o639dUWahUTdbKbiE7_GN2Ktb0WNKMZzoZwX40Czl714IPYaj9UWBqwfYwb0YKVsYtD3C_-Qe_PbqTWMiagTWPjvWoQFecsGzjOeaTm91DxqSE2Lg5Vo6N94vB64hCZAL_CWvbei-WXz__PXnyfsi8P2BZkF1pvu34YQT8Fsz0Yx8p7B81dkTESw07cVkMMOyHGnYhuzw4bdOd0uwT0DwcvCfc |
CitedBy_id | crossref_primary_10_1186_s13578_022_00887_3 crossref_primary_10_1126_sciadv_abb3298 crossref_primary_10_1371_journal_pone_0112122 crossref_primary_10_1016_j_actbio_2016_03_017 crossref_primary_10_1177_2472555217693191 crossref_primary_10_1186_s12935_015_0174_4 crossref_primary_10_3390_molecules28155719 crossref_primary_10_1155_2018_2856518 crossref_primary_10_1021_la500402m crossref_primary_10_1146_annurev_bioeng_071811_150028 crossref_primary_10_1038_srep17814 crossref_primary_10_1007_s40610_017_0073_7 crossref_primary_10_1021_acsbiomaterials_6b00218 crossref_primary_10_1016_j_biomaterials_2018_10_014 crossref_primary_10_1186_s40064_016_2629_z crossref_primary_10_1016_j_drudis_2016_06_001 crossref_primary_10_1371_journal_pone_0111029 crossref_primary_10_3390_cancers12102754 crossref_primary_10_18632_oncotarget_6267 crossref_primary_10_1016_j_biomaterials_2015_02_124 crossref_primary_10_1080_15384101_2017_1356512 crossref_primary_10_1016_j_biomaterials_2014_04_090 crossref_primary_10_1186_1471_2407_14_154 crossref_primary_10_3389_fcell_2022_932510 crossref_primary_10_3390_cancers13061334 crossref_primary_10_1021_acs_biomac_6b01311 crossref_primary_10_3390_cancers9010010 crossref_primary_10_3389_fphy_2021_749830 crossref_primary_10_1002_jbm_a_36070 crossref_primary_10_1093_jrr_rrv018 crossref_primary_10_1038_s41416_018_0260_1 crossref_primary_10_18632_oncotarget_12647 crossref_primary_10_1002_stem_1978 crossref_primary_10_1002_adhm_201500258 crossref_primary_10_1179_1743280415Y_0000000003 crossref_primary_10_1002_adhm_201400102 crossref_primary_10_1021_bm4004496 crossref_primary_10_18632_oncotarget_2313 crossref_primary_10_3390_bios6010002 crossref_primary_10_1038_s41388_018_0488_5 crossref_primary_10_1002_btpr_2692 crossref_primary_10_1021_mp500141b crossref_primary_10_1021_acsbiomaterials_0c01530 crossref_primary_10_1016_j_addr_2014_05_006 crossref_primary_10_1080_14737140_2022_2000864 crossref_primary_10_1016_j_addr_2014_06_004 crossref_primary_10_1016_j_biomaterials_2019_119402 crossref_primary_10_1016_j_actbio_2022_05_011 crossref_primary_10_1039_c3ib20252f crossref_primary_10_1557_mrc_2017_86 crossref_primary_10_1002_pros_24496 crossref_primary_10_1016_j_jbiosc_2014_01_008 crossref_primary_10_1186_s12885_015_1164_6 crossref_primary_10_1016_j_addr_2014_06_001 crossref_primary_10_1530_ERC_12_0401 crossref_primary_10_1371_journal_pone_0081689 crossref_primary_10_1371_journal_pone_0158116 crossref_primary_10_1038_nrd_2016_175 crossref_primary_10_18632_oncotarget_6643 crossref_primary_10_2144_btn_2021_0058 crossref_primary_10_1021_acsbiomaterials_7b00139 crossref_primary_10_1002_adtp_201800108 crossref_primary_10_1038_srep44824 crossref_primary_10_1158_1541_7786_MCR_18_1147 crossref_primary_10_1089_ten_teb_2015_0567 crossref_primary_10_1016_j_bone_2014_02_001 crossref_primary_10_1039_C9SM01666J crossref_primary_10_1155_2014_921905 |
ContentType | Journal Article |
Copyright | COPYRIGHT 2012 Public Library of Science Sieh et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2012 Sieh et al 2012 Sieh et al |
Copyright_xml | – notice: COPYRIGHT 2012 Public Library of Science – notice: Sieh et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: 2012 Sieh et al 2012 Sieh et al |
DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION IOV ISR 3V. 7QG 7QL 7QO 7RV 7SN 7SS 7T5 7TG 7TM 7U9 7X2 7X7 7XB 88E 8AO 8C1 8FD 8FE 8FG 8FH 8FI 8FJ 8FK ABJCF ABUWG AFKRA ARAPS ATCPS AZQEC BBNVY BENPR BGLVJ BHPHI C1K CCPQU D1I DWQXO FR3 FYUFA GHDGH GNUQQ H94 HCIFZ K9. KB. KB0 KL. L6V LK8 M0K M0S M1P M7N M7P M7S NAPCQ P5Z P62 P64 PATMY PDBOC PIMPY PQEST PQQKQ PQUKI PRINS PTHSS PYCSY RC3 7X8 5PM DOA |
DOI | 10.1371/journal.pone.0040217 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Opposing Viewpoints Resource Center Science in Context ProQuest Central (Corporate) Animal Behavior Abstracts Bacteriology Abstracts (Microbiology B) Biotechnology Research Abstracts ProQuest Nursing & Allied Health Database Ecology Abstracts Entomology Abstracts (Full archive) Immunology Abstracts Meteorological & Geoastrophysical Abstracts Nucleic Acids Abstracts Virology and AIDS Abstracts Agricultural Science Collection Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) ProQuest Pharma Collection ProQuest Public Health Database Technology Research Database ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Collection Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) Materials Science & Engineering Collection ProQuest Central (Alumni) ProQuest Central Advanced Technologies & Aerospace Collection Agricultural & Environmental Science Collection ProQuest Central Essentials Biological Science Collection ProQuest Central Technology Collection Natural Science Collection Environmental Sciences and Pollution Management ProQuest One Community College ProQuest Materials Science Collection ProQuest Central Korea Engineering Research Database Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student AIDS and Cancer Research Abstracts SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) https://resources.nclive.org/materials Nursing & Allied Health Database (Alumni Edition) Meteorological & Geoastrophysical Abstracts - Academic ProQuest Engineering Collection Biological Sciences Agriculture Science Database Health & Medical Collection (Alumni Edition) PML(ProQuest Medical Library) Algology Mycology and Protozoology Abstracts (Microbiology C) Biological Science Database Engineering Database Nursing & Allied Health Premium Advanced Technologies & Aerospace Database ProQuest Advanced Technologies & Aerospace Collection Biotechnology and BioEngineering Abstracts Environmental Science Database Materials Science Collection Publicly Available Content (ProQuest) ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China Engineering Collection Environmental Science Collection Genetics Abstracts MEDLINE - Academic PubMed Central (Full Participant titles) Directory of Open Access Journals |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef Agricultural Science Database Publicly Available Content Database ProQuest Central Student ProQuest Advanced Technologies & Aerospace Collection ProQuest Central Essentials Nucleic Acids Abstracts SciTech Premium Collection ProQuest Central China Environmental Sciences and Pollution Management Health Research Premium Collection Meteorological & Geoastrophysical Abstracts Natural Science Collection Biological Science Collection ProQuest Medical Library (Alumni) Engineering Collection Advanced Technologies & Aerospace Collection Engineering Database Virology and AIDS Abstracts ProQuest Biological Science Collection ProQuest One Academic Eastern Edition Agricultural Science Collection ProQuest Hospital Collection ProQuest Technology Collection Health Research Premium Collection (Alumni) Biological Science Database Ecology Abstracts ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts Environmental Science Collection Entomology Abstracts Nursing & Allied Health Premium ProQuest Health & Medical Complete ProQuest One Academic UKI Edition Environmental Science Database ProQuest Nursing & Allied Health Source (Alumni) Engineering Research Database ProQuest One Academic Meteorological & Geoastrophysical Abstracts - Academic Technology Collection Technology Research Database Materials Science Collection ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest Natural Science Collection ProQuest Pharma Collection ProQuest Central Genetics Abstracts ProQuest Engineering Collection Biotechnology Research Abstracts Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) Agricultural & Environmental Science Collection AIDS and Cancer Research Abstracts Materials Science Database ProQuest Materials Science Collection ProQuest Public Health ProQuest Nursing & Allied Health Source ProQuest SciTech Collection Advanced Technologies & Aerospace Database ProQuest Medical Library Animal Behavior Abstracts Materials Science & Engineering Collection Immunology Abstracts ProQuest Central (Alumni) MEDLINE - Academic |
DatabaseTitleList | MEDLINE Agricultural Science Database |
Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 4 dbid: 8FG name: ProQuest Technology Collection url: https://search.proquest.com/technologycollection1 sourceTypes: Aggregation Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Sciences (General) Medicine Biology Engineering Chemistry |
DocumentTitleAlternate | Characterization of LNCaP Cells Grown in 3D |
EISSN | 1932-6203 |
Editor | Wilson, Elizabeth |
Editor_xml | – sequence: 1 givenname: Elizabeth surname: Wilson fullname: Wilson, Elizabeth |
EndPage | e40217 |
ExternalDocumentID | 1330884509 oai_doaj_org_article_3ae1c03cc7664588b81ef6f282d896d5 2949801951 A543308777 10_1371_journal_pone_0040217 22957009 |
Genre | Research Support, Non-U.S. Gov't Journal Article |
GeographicLocations | Canada Queensland Australia Vancouver British Columbia Canada Australia |
GeographicLocations_xml | – name: Canada – name: Vancouver British Columbia Canada – name: Queensland Australia – name: Australia |
GrantInformation_xml | – fundername: Medical Research Council |
GroupedDBID | --- 123 29O 2WC 3V. 53G 5VS 7RV 7X2 7X7 7XC 88E 8AO 8C1 8CJ 8FE 8FG 8FH 8FI 8FJ A8Z AAFWJ ABDBF ABIVO ABJCF ABUWG ACGFO ACIHN ACIWK ACPRK ADBBV ADRAZ AEAQA AENEX AFKRA AFRAH AHMBA ALIPV ALMA_UNASSIGNED_HOLDINGS AOIJS APEBS ARAPS ATCPS BAWUL BBNVY BBORY BCNDV BENPR BGLVJ BHPHI BKEYQ BPHCQ BVXVI BWKFM CCPQU CGR CS3 CUY CVF D1I D1J D1K DIK DU5 E3Z EAP EAS EBD ECM EIF EMOBN ESTFP ESX EX3 F5P FPL FYUFA GROUPED_DOAJ GX1 HCIFZ HH5 HMCUK HYE IAO IEA IHR IHW INH INR IOV IPNFZ IPY ISE ISR ITC K6- KB. KQ8 L6V LK5 LK8 M0K M1P M48 M7P M7R M7S M~E NAPCQ NPM O5R O5S OK1 P2P P62 PATMY PDBOC PIMPY PQQKQ PROAC PSQYO PTHSS PV9 PYCSY RIG RNS RPM RZL SV3 TR2 UKHRP WOQ WOW ~02 ~KM AAYXX CITATION AFPKN 7QG 7QL 7QO 7SN 7SS 7T5 7TG 7TM 7U9 7XB 8FD 8FK AZQEC C1K DWQXO FR3 GNUQQ H94 K9. KL. M7N P64 PQEST PQUKI PRINS RC3 7X8 5PM AAPBV ABPTK |
ID | FETCH-LOGICAL-c758t-6c6ea12507ed502ce03c38fbad52cfedaf2e52bb021a7403610709f995c1cd943 |
IEDL.DBID | RPM |
ISSN | 1932-6203 |
IngestDate | Sun Nov 05 00:21:01 EDT 2023 Tue Oct 22 15:16:21 EDT 2024 Tue Sep 17 21:09:35 EDT 2024 Fri Oct 25 01:19:16 EDT 2024 Thu Oct 10 17:02:14 EDT 2024 Tue Nov 19 21:04:52 EST 2024 Tue Nov 12 22:53:34 EST 2024 Thu Aug 01 20:10:12 EDT 2024 Thu Aug 01 20:09:03 EDT 2024 Tue Aug 20 22:10:03 EDT 2024 Thu Nov 21 23:08:50 EST 2024 Tue Oct 15 23:44:09 EDT 2024 |
IsDoiOpenAccess | true |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 9 |
Language | English |
License | This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. Creative Commons Attribution License |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c758t-6c6ea12507ed502ce03c38fbad52cfedaf2e52bb021a7403610709f995c1cd943 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Competing Interests: The authors have declared that no competing interests exist. Conceived and designed the experiments: SS AVT SCR JAC CCN DWH. Performed the experiments: SS AVT SCR. Analyzed the data: SS AVT MS MLL AR. Contributed reagents/materials/analysis tools: SS AVT SR MS AR JA MLL JAC CCN DWH. Wrote the paper: SS AVT MS MLL AR. Bioinformatics: MLL JA. |
OpenAccessLink | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3434144/ |
PMID | 22957009 |
PQID | 1330884509 |
PQPubID | 1436336 |
PageCount | e40217 |
ParticipantIDs | plos_journals_1330884509 doaj_primary_oai_doaj_org_article_3ae1c03cc7664588b81ef6f282d896d5 pubmedcentral_primary_oai_pubmedcentral_nih_gov_3434144 proquest_miscellaneous_1038592039 proquest_journals_1330884509 gale_infotracmisc_A543308777 gale_infotracacademiconefile_A543308777 gale_incontextgauss_ISR_A543308777 gale_incontextgauss_IOV_A543308777 gale_healthsolutions_A543308777 crossref_primary_10_1371_journal_pone_0040217 pubmed_primary_22957009 |
PublicationCentury | 2000 |
PublicationDate | 2012-09-05 |
PublicationDateYYYYMMDD | 2012-09-05 |
PublicationDate_xml | – month: 09 year: 2012 text: 2012-09-05 day: 05 |
PublicationDecade | 2010 |
PublicationPlace | United States |
PublicationPlace_xml | – name: United States – name: San Francisco – name: San Francisco, USA |
PublicationTitle | PloS one |
PublicationTitleAlternate | PLoS One |
PublicationYear | 2012 |
Publisher | Public Library of Science Public Library of Science (PLoS) |
Publisher_xml | – name: Public Library of Science – name: Public Library of Science (PLoS) |
References | 10385425 - Endocrinology. 1999 Jul;140(7):3282-91 16169468 - Cancer Cell. 2005 Sep;8(3):241-54 18512241 - Int J Cancer. 2008 Aug 15;123(4):760-8 12818555 - Biomaterials. 2003 Sep;24(21):3825-34 9255794 - Nat Biotechnol. 1997 Aug;15(8):778-83 18566957 - Biotechnol J. 2008 Oct;3(9-10):1172-84 12036948 - Cancer Res. 2002 Jun 1;62(11):3298-307 11586096 - Am J Clin Oncol. 2001 Oct;24(5):458-61 19126683 - Proc Natl Acad Sci U S A. 2009 Jan 13;106(2):399-404 17719539 - Cell. 2007 Aug 24;130(4):601-10 15753383 - Cancer Res. 2005 Mar 1;65(5):1849-57 19693011 - Nature. 2009 Sep 3;461(7260):109-13 21056661 - J Steroid Biochem Mol Biol. 2011 Jan;123(1-2):58-64 21197221 - Organogenesis. 2010 Jul-Sep;6(3):181-8 20097238 - J Biotechnol. 2010 Jul 1;148(1):3-15 9716373 - Eur J Biochem. 1998 Jul 15;255(2):329-35 20454659 - PLoS One. 2010;5(5):e10431 20684983 - Biomaterials. 2010 Nov;31(32):8454-64 18058370 - Int J Radiat Biol. 2007 Nov-Dec;83(11-12):849-71 17957715 - Biopolymers. 2008 Feb;89(2):135-43 20886123 - PLoS One. 2010;5(9):e12905 21886458 - J Carcinog. 2011;10:20 18957712 - Cancer Biomark. 2008;4(4-5):213-25 16456867 - Cytometry B Clin Cytom. 2006 Mar;70(2):45-55 20960462 - Int J Cancer. 2011 Feb 15;128(4):962-73 14967430 - Int J Radiat Oncol Biol Phys. 2004 Mar 1;58(3):750-7 9105051 - J Cell Biol. 1997 Apr 7;137(1):231-45 17440684 - Cancer Metastasis Rev. 2007 Jun;26(2):225-39 12231360 - Curr Opin Cell Biol. 2002 Oct;14(5):633-9 19247489 - PLoS One. 2009;4(2):e4632 19575657 - Annu Rev Cell Dev Biol. 2009;25:407-29 20056286 - Trends Biotechnol. 2010 Mar;28(3):125-33 21210057 - Integr Biol (Camb). 2011 Apr;3(4):267-78 20133591 - Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3722-7 21732405 - J Cell Biochem. 2011 Nov;112(11):3151-8 15143345 - Cancer Gene Ther. 2004 Jul;11(7):508-13 12741789 - Biomacromolecules. 2003 May-Jun;4(3):713-22 16707435 - Cancer Res. 2006 May 15;66(10):5121-9 11953828 - Br J Cancer. 2002 Mar 18;86(6):947-53 17568666 - Biomaterials. 2007 Sep;28(26):3856-66 19931152 - Cell. 2009 Nov 25;139(5):891-906 17767164 - Nat Methods. 2007 Oct;4(10):855-60 12837835 - J Natl Cancer Inst. 2003 Jul 2;95(13):990-1000 12202388 - Biophys J. 2002 Sep;83(3):1650-60 20929283 - Tissue Eng Part A. 2011 Mar;17(5-6):713-24 10541360 - Clin Cancer Res. 1999 Oct;5(10 Suppl):3171s-3176s 18287387 - CA Cancer J Clin. 2008 Mar-Apr;58(2):71-96 17352215 - Anticancer Res. 2007 Jan-Feb;27(1A):45-53 21084386 - Cold Spring Harb Perspect Biol. 2010 Dec;2(12):a005066 3871197 - J Biol Chem. 1985 Jan 10;260(1):455-61 21747118 - Cancer Res. 2011 Sep 1;71(17):5754-64 20709389 - Biomaterials. 2010 Nov;31(32):8494-506 PLoS One. 2013;8(1). doi:10.1371/annotation/a26f1a2e-2765-4c10-942e-d2a738b80bf9 21310825 - Cancer Res. 2011 Apr 1;71(7):2739-49 18384940 - Cancer Lett. 2008 Jun 18;264(2):172-80 12686696 - Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5413-8 2451290 - Science. 1988 Apr 8;240(4849):177-84 19546857 - Nat Rev Mol Cell Biol. 2009 Jul;10(7):445-57 21723391 - Matrix Biol. 2011 Sep;30(7-8):363-8 17129778 - Cell. 2006 Dec 1;127(5):905-15 15330157 - Anticancer Res. 2004 Jul-Aug;24(4):2169-77 15637621 - Nat Biotechnol. 2005 Jan;23(1):47-55 15082523 - Endocr Rev. 2004 Apr;25(2):276-308 16607289 - Nat Rev Mol Cell Biol. 2006 Apr;7(4):265-75 3689657 - Br J Cancer. 1987 Oct;56(4):395-400 19107869 - Prostate. 2009 Mar 1;69(4):428-42 21606178 - Clin Cancer Res. 2011 Jul 1;17(13):4414-24 18680382 - Mol Pharm. 2008 Sep-Oct;5(5):849-62 18307210 - Hepatology. 2008 Apr;47(4):1394-400 18676866 - Cancer Res. 2008 Aug 1;68(15):6407-15 17579212 - Mol Endocrinol. 2007 Sep;21(9):2071-84 19151763 - Oncogene. 2009 Mar 5;28(9):1248-60 19626122 - PLoS One. 2009;4(7):e6361 17900747 - Adv Drug Deliv Rev. 2007 Nov 10;59(13):1329-39 17582839 - Tissue Eng. 2007 May;13(5):1091-101 10197347 - Ultrason Imaging. 1998 Oct;20(4):260-74 19276168 - Mol Cancer Ther. 2009 Mar;8(3):499-508 11604064 - Radiat Res. 2001 Nov;156(5 Pt 1):510-20 16293750 - Science. 2005 Nov 18;310(5751):1139-43 17883273 - Biomacromolecules. 2007 Oct;8(10):3000-7 14760391 - Br J Cancer. 2004 Feb 9;90(3):728-35 17396127 - Nat Methods. 2007 Apr;4(4):359-65 12475912 - FASEB J. 2003 Jan;17(1):97-9 15191644 - J Biomol Screen. 2004 Jun;9(4):273-85 21465482 - J Cell Physiol. 2012 Feb;227(2):751-8 20473410 - Integr Biol (Camb). 2010 Jan;2(1):32-40 20082656 - J Cell Mol Med. 2010 Apr;14(4):1003-13 14755679 - J Cell Biochem. 2004 Feb 15;91(3):483-90 18524624 - Semin Cancer Biol. 2008 Oct;18(5):338-48 9851876 - Exp Cell Res. 1998 Dec 15;245(2):350-9 17670948 - Proc Natl Acad Sci U S A. 2007 Aug 7;104(32):13092-7 |
References_xml | |
SSID | ssj0053866 |
Score | 2.4012988 |
Snippet | Biophysical and biochemical properties of the microenvironment regulate cellular responses such as growth, differentiation, morphogenesis and migration in... |
SourceID | plos doaj pubmedcentral proquest gale crossref pubmed |
SourceType | Open Website Open Access Repository Aggregation Database Index Database |
StartPage | e40217 |
SubjectTerms | Active Transport, Cell Nucleus Amino Acid Motifs Androgen receptors Androgens Apoptosis Arginine Aspartic acid Binding sites Bioengineering Biology Biomimetics Biophysics - methods Cancer Cell culture Cell Culture Techniques Cell Differentiation Cell growth Cell Line, Tumor Chemistry Collagen Collagen - chemistry Cores Degradability Differentiation Diffusion DNA microarrays Drug Combinations Engineering Evolution & development Extracellular matrix Extracellular Matrix - metabolism Gene expression Glycine Growth kinetics Hospitals Humans Hydrogel, Polyethylene Glycol Dimethacrylate Hydrogels Hydrogels - chemistry Hypoxia Kinases Kinetics Laminin - chemistry Ligands Male Materials Science Matrix metalloproteinase Mechanical properties Medical prognosis Medical research Medicine Mens health Metalloproteinase Morphogenesis Morphology Nuclear transport Phenotype Polyethylene glycol Prostate cancer Prostatic Neoplasms - pathology Protein binding Proteins Proteoglycans - chemistry Proteolysis Receptors, Androgen - metabolism Stiffness Stimulation Stress, Mechanical Studies Time Factors Translocation |
SummonAdditionalLinks | – databaseName: Directory of Open Access Journals dbid: DOA link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Nb9QwELXQnnpBtAW60FKDkIBD2sR2nM2xVFQFoVIBRb1FjuNpV0ASNbsH_n1nYm_YoEpw4BpPomQ-4jfJzBvGXlqMGQCg5hiBCYpyOQ1yzyMtwMgMctzl-mqLM316oT5cppdro76oJszTA3vFHUrjEhtLazOtqauynCUONGCmUM1yXXn20liskin_DsYo1jo0ysksOQx2OWib2h2Q34p-QNnvjajn6x_eypP2R9PdBTn_rJxc24pOHrD7AUPyI3_vm-yeq7fYZojSjr8OVNJvttn382tXN4tf7dxyO1Az-85L3gBvqecD0Sa3ZPwb_vHs2Jxz-pjfcc_J4SpOn2rnNTe8nDcu0Bfi4Z9UyrfWJ_eQXZy8-3p8GoXxCpHFJGERaaudQXwTZ65KY0GDw6ycQWmqVFhwlQHhUlGWqCyTKdzpMFOMc8jz1Ca2ypV8xCY1KnSHcZFpsDFUiK0SZUuJabWAEhQkBhGmhSmLVrouWs-iUfS_0jLMPrzSCrJNEWwzZW_JIIMscWD3B9AziuAZxd88Y8r2yZyFbygdIrk4SpX0PIhT9qKXIB6Mmgptrsyy64r3n779g9CXzyOhV0EIGnQMa0JzAz4T8WuNJHdHkhjNdrS8Q8630kqHOsKFmUJch2euHPLu5efDMl2Uiudq1yxRhv7-5iKWKPPY---gWZrmnsV0djby7JHqxyv1_LqnIZcKEZBST_6HrZ6yDUSioi_eS3fZZHGzdHuI9hblsz6wbwFyUVQe priority: 102 providerName: Directory of Open Access Journals – databaseName: ProQuest Technology Collection dbid: 8FG link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1Lb9QwELZgEYgLosujCwUMQgIOaRPHcTYnVCKWgmhZAa16sxzHbleskrDZPfTfM5M4aYMqxDWevOZhz9gz3xDyWoPNWGuxOIZBgMJNgo3cE08wq8LYJrDKNdkWR-LgmH85jU7dhlvt0iq7ObGZqPNS4x75HsRSYBAc1rf31W8Pu0bh6aproXGT3ApYLDClbzr71M3EYMtCuHK5MA72nHR2q7Iwu6i9rGlTdrkcNaj9_dw8qpZlfZ3j-Xf-5JUFaXaf3HOeJN1vRb9FbphiTG63vSUvxuTOoTs1H5MtZ8A1fetQpt89IL_m56Yo1xfVQtO0R21uizJpaekcy0HAEaUp6sWKfj1K1ZymZrmsaYvDaXKKu7iLgioKrzUO2RAuH2KW35USuofkePbxZ3rguc4Lnob4Ye0JLYwC18ePTR75DHuK6XBqM5VHTFuTK8tMxLIMOKhiDosgBJF-YpMk0oHOEx4-IqMCuLxNKIjFat_m4HYFXGchRNzMZpbbQIHzqe2EeJ0AZNUCbMjmlC2GwKTlpESBSSewCfmAUuppER67uVCuzqSzNhkqE2j4Zh0LgaW42TQwVlgIL_NpIvJoQl6gjGVba9obudyPeNhCJE7Iq4YCITIKzME5U5u6lp-_nfwH0Y_vA6I3jsiWoC1auboH-CeE3hpQ7gwowdD1YHgbNbLjSi0vTQLu7LT0-uGX_TA-FPPqClNugAYPhhPmh0DzuFXqnrPY6D328e54oO4D1g9HisV5g1AecnCOOH_y7896Su6C-8majL1oh4zWq415Bi7eOnve2PEflFFRDQ priority: 102 providerName: ProQuest – databaseName: Scholars Portal Journals: Open Access dbid: M48 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1Rb9MwELZGeeEFsQ1YxxgGIQEPqRLHcZoHhMa0aSAYE1C0t8hxfFvFSELTSuzfc5c40YKKtNf4nDbnu_i7-O47xl4a9BkAoOIYgQGKtAk1ck88JUCHMSS4yzXZFqfqZCY_nkfnG6zr2eoUWK8N7aif1GxxNfnz-_odOvzbpmtDHHSTJlVZ2AlZJcLsO-yuwL2Rkrw-y_5cAb1bKVdA97-Zgw2q4fHv39aj6qqs10HRfzMqb2xRxw_YfYct-UFrDJtswxZbbNN5b81fO4rpN9vs59mlLcrldTU33PSUzW1FJi-BV1QLgiiUGzKKBf90eqjPOH3kr3nL1WFzTp9w5wXXPJuX1tEa4uVflOJ3o37uIZsdH30_PPFc2wXPYPCw9JRRViPu8WObR76ghmImnEKm80gYsLkGYSORZagsHUvcATGC9BNIksgEJk9k-IiNClToDuMiVmB8yBFzBdJkIYbbAjKQEGhEngbGzOt0nVYtu0baHLHFGJW0SktpbVK3NmP2nhaklyVu7OZCubhInaulobaBwf9sYqWoDjebBhYUYGyZTxOVR2P2jJYzbQtNew9PDyIZtvyIY_aikSB-jIIScC70qq7TD19-3ELo29eB0CsnBCUahtGu6AGfiXi3BpJ7A0n0cjMY3iHj67RSo45wYCoR7-HMziDXDz_vh-mmlFRX2HKFMnQqnAg_RJnHrf32mqUu77FPs-OBZQ9UPxwp5pcNPXkoERlJuXuL333C7iEAFU3OXrTHRsvFyj5FkLfM9hu__QsPclOd priority: 102 providerName: Scholars Portal |
Title | Phenotypic characterization of prostate cancer LNCaP cells cultured within a bioengineered microenvironment |
URI | https://www.ncbi.nlm.nih.gov/pubmed/22957009 https://www.proquest.com/docview/1330884509 https://search.proquest.com/docview/1038592039 https://pubmed.ncbi.nlm.nih.gov/PMC3434144 https://doaj.org/article/3ae1c03cc7664588b81ef6f282d896d5 http://dx.doi.org/10.1371/journal.pone.0040217 |
Volume | 7 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9NAEB61QbwOiIZHAyUsCAk4OInt9evYWg0FkRAVWuVm2evdNiK1ozg59N8zY6-tGPWAuOzBO7bX89id8c58C_BBoM0opag4xsIAhcuADnIPDNdSse2pAFe5Mtti6p5d8G9zZ74HTl0LUybti2QxyJY3g2xxXeZWrm7EsM4TG84moc1x7uV8uA_7uPzWIXo1_aIBu66ukbM9c6hFMljlmRyQyqIPTgjAVkDI7kFrOSpR-5u5ubNa5sVdjuff-ZM7C9L4KTzRniQ7rkZ8AHsy68L96mzJ2y48DOuj3LrweAd1sAsPJno_vQsH2rQL9knjT39-Br9n1zLLN7erhWBhg-dclWuyXLEZFYqgi8pC0pg1-z4N4xkL5XJZsAqhU6aM_u8uMhYzHJDUb8fLE8r_2ymuew4X49Nf4Zmhz2QwBEYWG8MVrozRKRp5MnVGFp02JmxfJXHqWELJNFaWdKwkQTbHHsflEcPLUaCCwBGmSANuv4BOhqI4BGZ5rhIjlaJDZnKR2BiLWypRXJkxuqVC9cCoRROtKuiNqNx_8zBkqXgckVQjLdUenJD8GloCzi4v5OurSKtPZMfSFDhm4bkuFekmvimVqzDwTP3ATZ0evCXpR1UVamP-0bHD7Qo8sQfvSwoCz8goO-cq3hZF9PXH5T8Q_TxvEX3URCpHPRKxrojAbyJQrhblUYsS1Ue0ug9JV2uuFMgj7PA5OoN4Z62_d3e_a7rpoZRxl8l8izS0ZRxYIxtpXlbq3nC2Np4eeC1DaLG-3YOmXGKXa9N99d93voZH6LNaZZqfcwSdzXor36BfuEn6OBvMPWz90KR2_KUP905Op7PzfvmnBdsJ9_vlbPEHFfNoWA |
link.rule.ids | 230,314,727,780,784,864,885,2102,2221,12056,12223,12765,21388,24318,27924,27925,31719,31720,33266,33267,33373,33374,33744,33745,43310,43579,43600,43805,53791,53793,73745,74014,74035,74302 |
linkProvider | National Library of Medicine |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV3db9MwELegiI8XxMrHCoMZhAQ8ZEscx2me0IiYOmjLBBvam-U49lZRJaVpH_bfc5e42YImxGt8aZP7yN3Zd78j5K0Gm7HWYnMMgwSFmwQHuSeeYFaFsU3Ay9XVFlMxOuVfzqIzt-FWubLKzTex_lDnpcY98n3IpcAgOPi3j4vfHk6NwtNVN0LjNrkDbj9GrR6mbYkH2LIQrl0ujIN9J529RVmYPdReVo8pu3JHNWp_-23uLeZldVPg-Xf95DWHdPiIPHSRJD1oRL9FbpmiT-42syUv--TexJ2a98mWM-CKvnco0x8ek1_HF6YoV5eLmaZpi9rcNGXS0tJjbAeBQJSmqBdLOp6m6pimZj6vaIPDaXKKu7izgioKf2scsiFcnmCV37UWuifk9PDzSTry3OQFT0P-sPKEFkZB6OPHJo98hjPFdDi0mcojpq3JlWUmYlkGHFQxBycISaSf2CSJdKDzhIdPSa8ALm8TymJhtW9zCLsCrrMQMm5mM8ttoCD41HZAvI0A5KIB2JD1KVsMiUnDSYkCk05gA_IJpdTSIjx2faFcnktnbTJUJtDwzDoWAltxs2FgrLCQXubDROTRgOyijGXTa9oauTyIeNhAJA7Im5oCITIKrME5V-uqkkfffv4H0Y_vHaJ3jsiWoC1aub4HeCeE3upQ7nQowdB1Z3kbNXLDlUpemQTcudHSm5dft8v4o1hXV5hyDTR4MJwwPwSaZ41St5zFQe-xj3fHHXXvsL67UswuaoTykENwxPnzfz_WLrk_OpmM5fho-vUFeQChKKur96Id0lst1-YlhHur7FVt038APghTzA |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV3db9MwELegiIkXxMrHCoMZhAQ8ZE2cxGme0AhUG2ylAob2ZjmOvVVUSWnah_333DlutqAJ8Rpfvu7DvrPvfkfIawU2Y4zB4hgGAUqkU2zknnqcGRkmJoVVzmZbTPjhafT5LD5z-U-1S6vczIl2oi4qhXvkQ4ilwCAiWN-GxqVFTD-O3y9-e9hBCk9aXTuN2-QOdplDHP1R1qZ7gF1z7krnwiQYOkntL6pS76MmM9uy7Gppsgj-7TzdW8yr-iYn9O9cymuL0_gBue-8SnrQqME2uaXLPrnb9Jm87JOtE3eC3ifbzphr-tYhTr97SH5NL3RZrS4XM0WzFsG5KdCklaFTLA0Bp5RmqCNLejzJ5JRmej6vaYPJqQuKO7qzkkoKr9UO5RAun2DG37VyukfkdPzpR3bouS4MnoJYYuVxxbUEN8hPdBH7DPuLqXBkclnETBldSMN0zPIcOCiB8SH4Y4mfmjSNVaCKNAofk14JXN4hlCXcKN8U4IIFkcpDiL6ZyU1kAgmOqDID4m0EIBYN2IawJ24JBCkNJwUKTDiBDcgHlFJLi1DZ9kK1PBfO8kQodaDgm1XCOZbl5qNAG24g1CxGKS_iAdlDGYum7rQ1eHEQR2EDlzggrywFwmWUqHjncl3X4ujrz_8g-v6tQ_TGEZkKtEVJVwMB_4QwXB3K3Q4lGL3qDO-gRm64Uosr84A7N1p68_DLdhgfijl2pa7WQIOHxCnzQ6B50ih1y1ls-p74eHfSUfcO67sj5ezCopWHEThKUfT035-1R7bAnMXx0eTLM3IPvFJmE_niXdJbLdf6OXh-q_yFNek_HFhX8Q |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Phenotypic+characterization+of+prostate+cancer+LNCaP+cells+cultured+within+a+bioengineered+microenvironment&rft.jtitle=PloS+one&rft.au=Sieh%2C+Shirly&rft.au=Taubenberger%2C+Anna+V&rft.au=Rizzi%2C+Simone+C&rft.au=Sadowski%2C+Martin&rft.date=2012-09-05&rft.eissn=1932-6203&rft.volume=7&rft.issue=9&rft.spage=e40217&rft.epage=e40217&rft_id=info:doi/10.1371%2Fjournal.pone.0040217&rft.externalDBID=NO_FULL_TEXT |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1932-6203&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1932-6203&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1932-6203&client=summon |