Basophil Activation Test Using Recombinant Allergens: Highly Specific Diagnostic Method Complementing Routine Tests in Wasp Venom Allergy

• Published in: PloS one Vol. 9; no. 10; p. e108619
• Main Authors: Balzer, Lukas, Pennino, Davide, Blank, Simon, Seismann, Henning, Darsow, Ulf, Schnedler, Mathias, McIntyre, Mareike, Ollert, Markus W., Durham, Stephen R., Spillner, Edzard, Ring, Johannes, Cifuentes, Liliana
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 17.10.2014; Public Library of Science (PLoS)
• Subjects: Activation; Adult; Allergens; Allergens - genetics; Allergens - immunology; Allergies; Allergy; Analysis; Anaphylaxis; Anaphylaxis - diagnosis; Anaphylaxis - immunology; Antigens; Basophils - immunology; Biology and Life Sciences; Case-Control Studies; Continuous improvement; Data processing; Dermatology; Diagnosis; Diagnostic reagents; Diagnostic systems; Enzyme-linked immunosorbent assay; Evaluation; Female; Humans; Hymenoptera; Hypersensitivity; Immunoglobulin E; Immunologic Tests - methods; Immunology; Immunotherapy; In vitro methods and tests; Insect bites; Male; Medical diagnosis; Medicine and Health Sciences; Methods; Middle Aged; Patients; Proteins; Recombinant Proteins - genetics; Recombinant Proteins - immunology; Sensitivity; Sensitivity and Specificity; Silicon compounds; Skin; Skin tests; Stings; Venom; Vespula; Wasp Venoms - genetics; Wasp Venoms - immunology; Wasps; Denmark; United Kingdom > UK; Switzerland; Aarhus Denmark; Germany

Skin testing can expose allergic subjects to potential systemic reactions, sensitization against unrelated proteins, and increased risk of future sting reactions. Therefore the continuous improvement of in vitro diagnostic methods is desirable. Recombinant allergens have been shown to improve the se...