Creation of Functional Viruses from Non-Functional cDNA Clones Obtained from an RNA Virus Population by the Use of Ancestral Reconstruction

• Published in: PloS one Vol. 10; no. 10; p. e0140912
• Main Authors: Fahnøe, Ulrik, Pedersen, Anders Gorm, Dräger, Carolin, Orton, Richard J, Blome, Sandra, Höper, Dirk, Beer, Martin, Rasmussen, Thomas Bruun
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 20.10.2015; Public Library of Science (PLoS)
• Subjects: Analysis; Animal welfare; Artificial chromosomes; Bioinformatics; Cell culture; Classical Swine Fever Virus - genetics; Cloning; Cloning, Molecular; Computer applications; DNA, Complementary - genetics; DNA, Viral - genetics; Evolution; Evolution, Molecular; Fever; Fitness; Gene sequencing; Genetic aspects; Genome, Viral; Genomes; Genomics; Haplotypes; Hog cholera; Hogs; In vivo methods and tests; Infections; Life sciences; Livestock; Mutation; Mutation rates; Phylogenetics; Phylogeny; Population; Reconstruction; Reproductive fitness; Ribonucleic acid; RNA; RNA viruses; RNA, Viral - genetics; Socio-economic aspects; Software; Sus scrofa; Swine; Swine production; Virology; Virulence; Virulence (Microbiology); Viruses; West Nile virus; United States; United Kingdom > UK; Denmark; Germany

RNA viruses have the highest known mutation rates. Consequently it is likely that a high proportion of individual RNA virus genomes, isolated from an infected host, will contain lethal mutations and be non-functional. This is problematic if the aim is to clone and investigate high-fitness, functiona...