Characterization of 4-HNE modified L-FABP reveals alterations in structural and functional dynamics

• Published in: PloS one Vol. 7; no. 6; p. e38459
• Main Authors: Smathers, Rebecca L, Fritz, Kristofer S, Galligan, James J, Shearn, Colin T, Reigan, Philip, Marks, Michael J, Petersen, Dennis R
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 06.06.2012; Public Library of Science (PLoS)
• Subjects: 4-Hydroxynonenal; Adipocytes; Alcohol; Aldehydes; Aldehydes - metabolism; Alterations; Amino acids; Analysis; Anilino Naphthalenesulfonates - metabolism; Animals; Biochemistry; Biology; Carbonyls; Chemistry; Cloning, Molecular; Compartments; Computer applications; Computer Science; Computer simulation; Deoxyribonucleic acid; DNA; DNA Primers - genetics; Electrophoresis, Gel, Two-Dimensional; Ethanol; Ethanol - adverse effects; Fatty acid-binding protein; Fatty Acid-Binding Proteins - chemistry; Fatty Acid-Binding Proteins - isolation & purification; Fatty Acid-Binding Proteins - metabolism; Fatty acids; Fatty liver; Fluorescence; Gene Expression Regulation - drug effects; Homeostasis; Hydrophobicity; Immunoprecipitation; Ligands; Lipid peroxidation; Lipids; Liver; Liver diseases; Liver Diseases, Alcoholic - metabolism; Liver Diseases, Alcoholic - physiopathology; Male; Mass Spectrometry; Mass spectroscopy; Medicine; Metabolism; Mice; Mice, Inbred C57BL; Models, Molecular; Molecular modelling; mRNA; Oxidative stress; Pathogenesis; Peroxidation; Pharmaceutical sciences; Pharmacy; Polyunsaturated fatty acids; Protein Binding; Protein Conformation; Protein structure; Proteins; Proteomics - methods; Reverse Transcriptase Polymerase Chain Reaction; RNA; Rodents; Structural integrity; Structure-function relationships; Sulfonic acid; Target recognition; Thermal stability; Type 2 diabetes; Ubiquitination; Unsaturated fatty acids; United States > US; Colorado

4-Hydroxynonenal (4-HNE) is a reactive α,β-unsaturated aldehyde produced during oxidative stress and subsequent lipid peroxidation of polyunsaturated fatty acids. The reactivity of 4-HNE towards DNA and nucleophilic amino acids has been well established. In this report, using proteomic approaches, l...