Human B-cell isotype switching origins of IgE

• Published in: Journal of allergy and clinical immunology Vol. 137; no. 2; pp. 579 - 586.e7
• Main Authors: Looney, Timothy J., Lee, Ji-Yeun, Roskin, Krishna M., Hoh, Ramona A., King, Jasmine, Glanville, Jacob, Liu, Yi, Pham, Tho D., Dekker, Cornelia L., Davis, Mark M., Boyd, Scott D.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.02.2016; Elsevier Limited
• Subjects: Adult; Aged; Aged, 80 and over; Allergies; Allergy and Immunology; antibody; Antigens; B-Lymphocytes - immunology; B-Lymphocytes - metabolism; Base Sequence; B cell; Case-Control Studies; Cluster Analysis; Computational Biology - methods; direct; Female; high-throughput DNA sequencing; High-Throughput Nucleotide Sequencing; Human subjects; Humans; Hypersensitivity - genetics; Hypersensitivity - immunology; IgE; Immunoglobulin Class Switching - genetics; Immunoglobulin Class Switching - immunology; Immunoglobulin E - genetics; Immunoglobulin E - immunology; Immunoglobulin Heavy Chains - genetics; Immunoglobulin Isotypes - genetics; Immunoglobulin Isotypes - immunology; Immunoglobulins; indirect; Influenza; isotype switching; Library collections; Male; Middle Aged; Molecular Sequence Annotation; Molecular Sequence Data; Mutation; repertoire; Sequence Alignment; Somatic Hypermutation, Immunoglobulin; Young Adult; antibody; indirect; IgE; direct; repertoire; IGH; high-throughput DNA sequencing; FR; B cell; CDR3; isotype switching; Complementarity determining region 3; Immunoglobulin heavy chain gene; Framework region

B cells expressing IgE contribute to immunity against parasites and venoms and are the source of antigen specificity in allergic patients, yet the developmental pathways producing these B cells in human subjects remain a subject of debate. Much of our knowledge of IgE lineage development derives fro...