The Impact of Entropy on the Spatial Organization of Synaptonemal Complexes within the Cell Nucleus

• Published in: PloS one Vol. 7; no. 5; p. e36282
• Main Authors: Fritsche, Miriam, Reinholdt, Laura G., Lessard, Mark, Handel, Mary Ann, Bewersdorf, Jörg, Heermann, Dieter W.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 04.05.2012; Public Library of Science (PLoS)
• Subjects: Actin; Animals; Biology; Biopolymers; Cables; Cell division; Chromosomes; Chromosomes - metabolism; Confining; Deoxyribonucleic acid; DNA; Entanglement; Entropy; Imaging, Three-Dimensional; Laboratories; Male; Mathematical models; Meiosis; Mice; Microscopy; Nuclei; Nuclei (cytology); Numerical models; Pachytene; Physics; Polymers; Proteins; Rigidity; Spatial distribution; Spermatocytes - cytology; Studies; Synaptonemal Complex - metabolism; Tethering; Theoretical physics; Three dimensional models; United States > US; Maine; Germany
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0036282

We employ 4Pi-microscopy to study SC organization in mouse spermatocyte nuclei allowing for the three-dimensional reconstruction of the SC's backbone arrangement. Additionally, we model the SCs in the cell nucleus by confined, self-avoiding polymers, whose chain ends are attached to the envelop...