Patient-Specific and Genome-Edited Induced Pluripotent Stem Cell–Derived Cardiomyocytes Elucidate Single-Cell Phenotype of Brugada Syndrome - Liang, Ping; Sallam, Karim; Wu, Haodi; Li, Yingxin; Itzhaki, Ilanit; Garg, Priyanka; Zhang, Ying; Termglichan, Vittavat; Lan, Feng; Gu, Mingxia; Gong, Tingyu; Zhuge, Yan; et al. (Journal of the American College of Cardiology, 2016)
Keywords: action potential; Adolescent; Adult; arrhythmia; Brugada Syndrome - genetics; Brugada Syndrome - metabolism; Brugada Syndrome - pathology; Ca2+ transient; Cardiology; Cardiomyocytes; Cardiomyopathy; Cardiovascular; Cell adhesion & migration; Cell Differentiation; Editing; Electrocardiography; Family medical history; Fibroblasts; Gene expression; Gene Expression Regulation; Genetic engineering; genome editing; Genomes; Genotype; Genotype & phenotype; Heart Conduction System - pathology; Heart Conduction System - physiopathology; Humans; Hypotheses; Induced Pluripotent Stem Cells - cytology; Induced Pluripotent Stem Cells - metabolism; Male; Methods; Middle Aged; Mutation; Myocytes, Cardiac - cytology; Myocytes, Cardiac - metabolism; NAV1.5 Voltage-Gated Sodium Channel - biosynthesis; NAV1.5 Voltage-Gated Sodium Channel - genetics; Patients; Pedigree; Phenotype; Polymerase Chain Reaction; RNA - genetics; SCN5A; Sendai virus; Sodium; Stem cells; Studies; Young Adult; genome editing; BrS; iPSC-CM; Vmax; iPSC; ECG; action potential; AP; hNav1.5; arrhythmia; Ca2+ transient; SCN5A; INa; gene expression; human sodium channel protein type 5 subunit alpha; Ca 2+ transient; I Na; sodium voltage-gated channel alpha subunit 5; electrocardiogram; type 1 Brugada syndrome; induced pluripotent stem cell; maximal upstroke velocity; V max; hNa v1.5; sodium current; induced pluripotent stem cell–derived cardiomyocyte; Ca(2+) transient