Evaluation of estrogenic potential of flavonoids using a recombinant yeast strain and MCF7/BUS cell proliferation assay
Phytoestrogens are of interest because of their reported beneficial effects on many human maladies including cancer, neurodegeneration, cardiovascular disease and diabetes. Furthermore, there is a search for compounds with estrogenic activity that can replace estrogen in hormone replacement therapy...
Saved in:
Published in | PloS one Vol. 8; no. 10; p. e74881 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Public Library of Science
01.10.2013
Public Library of Science (PLoS) |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Phytoestrogens are of interest because of their reported beneficial effects on many human maladies including cancer, neurodegeneration, cardiovascular disease and diabetes. Furthermore, there is a search for compounds with estrogenic activity that can replace estrogen in hormone replacement therapy during menopause, without the undesirable effects of estrogen, such as the elevation of breast cancer occurrence. Thus, the principal objective of this study was to assess the estrogenic activity of flavonoids with different hydroxylation patterns: quercetin, kaempferol, luteolin, fisetin, chrysin, galangin, flavone, 3-hydroxyflavone, 5-hydroxyflavone and 7-hydroxyflavone via two different in vitro assays, the recombinant yeast assay (RYA) and the MCF-7 proliferation assay (E-screen), since the most potent phytoestrogens are members of the flavonoid family. In these assays, kaempferol was the only compound that showed ERα-dependent transcriptional activation activity by RYA, showing 6.74±1.7 nM EEQ, besides acting as a full agonist for the stimulation of proliferation of MCF-7/BUS cells. The other compounds did not show detectable levels of interaction with ER under the conditions used in the RYA. However, in the E-screen assay, compounds such as galangin, luteolin and fisetin also stimulated the proliferation of MCF-7/BUS cells, acting as partial agonists. In the evaluation of antiestrogenicity, the compounds quercetin, chrysin and 3-hydroxyflavone significantly inhibited the cell proliferation induced by 17-β-estradiol in the E-screen assay, indicating that these compounds may act as estrogen receptor antagonists. Overall, it became clear in the assay results that the estrogenic activity of flavonoids was affected by small structural differences such as the number of hydroxyl groups, especially those on the B ring of the flavonoid. |
---|---|
AbstractList | Phytoestrogens are of interest because of their reported beneficial effects on many human maladies including cancer, neurodegeneration, cardiovascular disease and diabetes. Furthermore, there is a search for compounds with estrogenic activity that can replace estrogen in hormone replacement therapy during menopause, without the undesirable effects of estrogen, such as the elevation of breast cancer occurrence. Thus, the principal objective of this study was to assess the estrogenic activity of flavonoids with different hydroxylation patterns: quercetin, kaempferol, luteolin, fisetin, chrysin, galangin, flavone, 3-hydroxyflavone, 5-hydroxyflavone and 7-hydroxyflavone via two different in vitro assays, the recombinant yeast assay (RYA) and the MCF-7 proliferation assay (E-screen), since the most potent phytoestrogens are members of the flavonoid family. In these assays, kaempferol was the only compound that showed ERα-dependent transcriptional activation activity by RYA, showing 6.74±1.7 nM EEQ, besides acting as a full agonist for the stimulation of proliferation of MCF-7/BUS cells. The other compounds did not show detectable levels of interaction with ER under the conditions used in the RYA. However, in the E-screen assay, compounds such as galangin, luteolin and fisetin also stimulated the proliferation of MCF-7/BUS cells, acting as partial agonists. In the evaluation of antiestrogenicity, the compounds quercetin, chrysin and 3-hydroxyflavone significantly inhibited the cell proliferation induced by 17-β-estradiol in the E-screen assay, indicating that these compounds may act as estrogen receptor antagonists. Overall, it became clear in the assay results that the estrogenic activity of flavonoids was affected by small structural differences such as the number of hydroxyl groups, especially those on the B ring of the flavonoid. Phytoestrogens are of interest because of their reported beneficial effects on many human maladies including cancer, neurodegeneration, cardiovascular disease and diabetes. Furthermore, there is a search for compounds with estrogenic activity that can replace estrogen in hormone replacement therapy during menopause, without the undesirable effects of estrogen, such as the elevation of breast cancer occurrence. Thus, the principal objective of this study was to assess the estrogenic activity of flavonoids with different hydroxylation patterns: quercetin, kaempferol, luteolin, fisetin, chrysin, galangin, flavone, 3-hydroxyflavone, 5-hydroxyflavone and 7-hydroxyflavone via two different in vitro assays, the recombinant yeast assay (RYA) and the MCF-7 proliferation assay (E-screen), since the most potent phytoestrogens are members of the flavonoid family. In these assays, kaempferol was the only compound that showed ER[alpha]-dependent transcriptional activation activity by RYA, showing 6.74±1.7 nM EEQ, besides acting as a full agonist for the stimulation of proliferation of MCF-7/BUS cells. The other compounds did not show detectable levels of interaction with ER under the conditions used in the RYA. However, in the E-screen assay, compounds such as galangin, luteolin and fisetin also stimulated the proliferation of MCF-7/BUS cells, acting as partial agonists. In the evaluation of antiestrogenicity, the compounds quercetin, chrysin and 3-hydroxyflavone significantly inhibited the cell proliferation induced by 17-[beta]-estradiol in the E-screen assay, indicating that these compounds may act as estrogen receptor antagonists. Overall, it became clear in the assay results that the estrogenic activity of flavonoids was affected by small structural differences such as the number of hydroxyl groups, especially those on the B ring of the flavonoid. Phytoestrogens are of interest because of their reported beneficial effects on many human maladies including cancer, neurodegeneration, cardiovascular disease and diabetes. Furthermore, there is a search for compounds with estrogenic activity that can replace estrogen in hormone replacement therapy during menopause, without the undesirable effects of estrogen, such as the elevation of breast cancer occurrence. Thus, the principal objective of this study was to assess the estrogenic activity of flavonoids with different hydroxylation patterns: quercetin, kaempferol, luteolin, fisetin, chrysin, galangin, flavone, 3-hydroxyflavone, 5-hydroxyflavone and 7-hydroxyflavone via two different in vitro assays, the recombinant yeast assay (RYA) and the MCF-7 proliferation assay ( E-screen ), since the most potent phytoestrogens are members of the flavonoid family. In these assays, kaempferol was the only compound that showed ERα-dependent transcriptional activation activity by RYA, showing 6.74±1.7 nM EEQ, besides acting as a full agonist for the stimulation of proliferation of MCF-7/BUS cells. The other compounds did not show detectable levels of interaction with ER under the conditions used in the RYA. However, in the E-screen assay, compounds such as galangin, luteolin and fisetin also stimulated the proliferation of MCF-7/BUS cells, acting as partial agonists. In the evaluation of antiestrogenicity, the compounds quercetin, chrysin and 3-hydroxyflavone significantly inhibited the cell proliferation induced by 17-β-estradiol in the E-screen assay, indicating that these compounds may act as estrogen receptor antagonists. Overall, it became clear in the assay results that the estrogenic activity of flavonoids was affected by small structural differences such as the number of hydroxyl groups, especially those on the B ring of the flavonoid. |
Audience | Academic |
Author | de Camargo, Mariana S Varanda, Eliana A Resende, Flávia A Vilegas, Wagner de Oliveira, Ana Paula S |
AuthorAffiliation | Michigan State University, United States of America 1 Department of Biological Sciences, Faculty of Pharmaceutical Sciences, Univ Estadual Paulista, Araraquara, São Paulo, Brazil 2 Campus do Litoral Paulista-Unidade São Vicente, Univ Estadual Paulista, São Vicente, São Paulo, Brazil |
AuthorAffiliation_xml | – name: 1 Department of Biological Sciences, Faculty of Pharmaceutical Sciences, Univ Estadual Paulista, Araraquara, São Paulo, Brazil – name: 2 Campus do Litoral Paulista-Unidade São Vicente, Univ Estadual Paulista, São Vicente, São Paulo, Brazil – name: Michigan State University, United States of America |
Author_xml | – sequence: 1 givenname: Flávia A surname: Resende fullname: Resende, Flávia A organization: Department of Biological Sciences, Faculty of Pharmaceutical Sciences, Univ Estadual Paulista, Araraquara, São Paulo, Brazil – sequence: 2 givenname: Ana Paula S surname: de Oliveira fullname: de Oliveira, Ana Paula S – sequence: 3 givenname: Mariana S surname: de Camargo fullname: de Camargo, Mariana S – sequence: 4 givenname: Wagner surname: Vilegas fullname: Vilegas, Wagner – sequence: 5 givenname: Eliana A surname: Varanda fullname: Varanda, Eliana A |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/24098354$$D View this record in MEDLINE/PubMed |
BookMark | eNqNk19v0zAUxSM0xP7AN0AQCQnBQzs7dmL3BWlUG1QamsQYr9aN7bSuXLuzk0K_PQ7NphbtAeUh0c3vnGsf-55mR847nWWvMRpjwvD50nfBgR2vU3mMEKOc42fZCZ6QYlQViBztfR9npzEuESoJr6oX2XFB0YSTkp5kvy43YDtojXe5b3Id2-Dn2hmZr32rXWvA9vXGwsY7b1TMu2jcPIc8aOlXtXHg2nyrIbZ50oJxOTiVf5tesfPPd7e51Nbm6-CtaXTYtYEYYfsye96AjfrV8D7L7q4uf0y_jq5vvsymF9cjyUrejgqsKqIKKhGXhVJVVXDKawmYF5hQwDUHJlXBFCjeIEyAEFnokpeKsElTa3KWvd35rq2PYsgsCkwJZwhRWiRitiOUh6VYB7OCsBUejPhb8GEuILRGWi0YRwwU5aAbSqHSnDe0IZjUoJjiuE5en4ZuXb3SSqYAA9gD08M_zizE3G8EYZyjkieDD4NB8PddOg2xMrHPEJz2Xb9uSiguMUIJffcP-vTuBmoOaQPGNT71lb2puKCMU1pVfJKo8RNUepReGZkuWGNS_UDw8UCQmFb_bufQxShmt9__n735eci-32MXGmy7iN52_cWJhyDdgTL4GINuHkPGSPTz8ZCG6OdDDPORZG_2D-hR9DAQ5A8tUQ3x |
CitedBy_id | crossref_primary_10_1016_j_envint_2015_05_012 crossref_primary_10_1016_j_jep_2023_116325 crossref_primary_10_2478_aoas_2021_0040 crossref_primary_10_3389_fphar_2023_1122031 crossref_primary_10_3945_jn_114_198598 crossref_primary_10_1016_j_yrtph_2017_08_010 crossref_primary_10_1124_pr_115_010843 crossref_primary_10_3390_ijerph110807725 crossref_primary_10_1016_j_jnutbio_2022_109250 crossref_primary_10_1186_s43094_024_00623_3 crossref_primary_10_1002_cbin_11102 crossref_primary_10_3390_biom12040582 crossref_primary_10_1002_ptr_7855 crossref_primary_10_3390_nu12010059 crossref_primary_10_1097_GME_0000000000000395 crossref_primary_10_1016_j_foodres_2014_01_051 crossref_primary_10_1530_REP_17_0530 crossref_primary_10_1016_j_tifs_2019_11_014 crossref_primary_10_3390_ijms22137178 crossref_primary_10_3390_molecules25153531 crossref_primary_10_3892_or_2014_3627 crossref_primary_10_1515_jbcpp_2019_0032 crossref_primary_10_1038_s41598_019_43768_5 crossref_primary_10_1016_j_biopha_2018_09_154 crossref_primary_10_1016_j_jpba_2023_115241 crossref_primary_10_1002_mnfr_202100976 crossref_primary_10_1007_s12012_017_9420_4 crossref_primary_10_1371_journal_pone_0192490 crossref_primary_10_1002_mnfr_201400515 crossref_primary_10_2166_wh_2023_153 crossref_primary_10_2174_1385272824999201126214150 crossref_primary_10_1021_acs_jcim_1c01077 crossref_primary_10_1155_2017_7829059 crossref_primary_10_1111_nmo_14093 crossref_primary_10_1016_j_jep_2016_11_038 crossref_primary_10_1016_j_fitote_2017_11_003 crossref_primary_10_1038_srep32756 crossref_primary_10_1016_j_taap_2017_10_013 crossref_primary_10_1080_87559129_2021_2003380 crossref_primary_10_1016_j_foodres_2019_108715 crossref_primary_10_3109_13880209_2015_1133657 crossref_primary_10_1038_s41598_020_65403_4 crossref_primary_10_1080_26895293_2022_2147225 crossref_primary_10_1016_j_intimp_2018_06_016 crossref_primary_10_1016_j_rechem_2024_101544 crossref_primary_10_1186_s12906_016_1385_5 crossref_primary_10_1186_s12906_017_1895_9 crossref_primary_10_1016_j_biopha_2017_03_069 crossref_primary_10_3390_molecules25214914 crossref_primary_10_1248_bpb_b14_00780 crossref_primary_10_3390_ijms22168798 crossref_primary_10_1016_j_abb_2014_03_003 crossref_primary_10_1016_j_dental_2019_11_016 crossref_primary_10_1016_j_jclepro_2021_128202 crossref_primary_10_1186_s12906_017_1568_8 crossref_primary_10_1016_j_biopha_2021_112257 crossref_primary_10_1021_acsomega_1c04239 crossref_primary_10_2174_1871520622666220324094033 crossref_primary_10_1016_j_chroma_2017_02_019 crossref_primary_10_1186_s12906_020_02960_1 crossref_primary_10_1007_s43450_022_00238_w crossref_primary_10_1016_j_ab_2024_115529 crossref_primary_10_3389_fphar_2022_832928 crossref_primary_10_3390_ijerph13040373 |
Cites_doi | 10.1021/tx000208y 10.1016/j.phymed.2005.06.003 10.1016/S0009-2797(99)00139-8 10.1007/s11356-009-0234-1 10.1089/ars.2009.2816 10.1055/s-2007-993760 10.1007/s12199-010-0146-1 10.1016/j.chemosphere.2005.03.082 10.1289/ehp.95103844 10.1016/j.bmc.2008.12.044 10.1897/03-141 10.1124/mol.112.084228 10.1016/j.envint.2010.02.004 10.1016/0076-6879(91)94028-B 10.1021/np040069a 10.1016/j.tiv.2013.03.003 10.1016/j.jsbmb.2013.01.003 10.1002/etc.5620200603 10.1016/j.abb.2010.05.018 10.1016/j.talanta.2005.09.044 10.1023/A:1006422302173 10.1210/endo.139.10.6216 10.1016/j.jsbmb.2011.03.002 10.1016/j.jmgm.2011.01.001 10.1016/j.tiv.2009.07.032 10.1016/S0039-128X(96)00242-5 10.1016/S0027-5107(01)00073-2 10.1016/j.scitotenv.2010.06.049 |
ContentType | Journal Article |
Copyright | COPYRIGHT 2013 Public Library of Science 2013 Resende et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2013 Resende et al 2013 Resende et al |
Copyright_xml | – notice: COPYRIGHT 2013 Public Library of Science – notice: 2013 Resende et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: 2013 Resende et al 2013 Resende et al |
DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION IOV ISR 3V. 7QG 7QL 7QO 7RV 7SN 7SS 7T5 7TG 7TM 7U9 7X2 7X7 7XB 88E 8AO 8C1 8FD 8FE 8FG 8FH 8FI 8FJ 8FK ABJCF ABUWG AFKRA ARAPS ATCPS AZQEC BBNVY BENPR BGLVJ BHPHI C1K CCPQU D1I DWQXO FR3 FYUFA GHDGH GNUQQ H94 HCIFZ K9. KB. KB0 KL. L6V LK8 M0K M0S M1P M7N M7P M7S NAPCQ P5Z P62 P64 PATMY PDBOC PIMPY PQEST PQQKQ PQUKI PRINS PTHSS PYCSY RC3 7X8 5PM DOA |
DOI | 10.1371/journal.pone.0074881 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Opposing Viewpoints Resource Center Gale In Context: Science ProQuest Central (Corporate) Animal Behavior Abstracts Bacteriology Abstracts (Microbiology B) Biotechnology Research Abstracts ProQuest Nursing & Allied Health Database Ecology Abstracts Entomology Abstracts (Full archive) Immunology Abstracts Meteorological & Geoastrophysical Abstracts Nucleic Acids Abstracts Virology and AIDS Abstracts Agricultural Science Collection ProQuest Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) ProQuest Pharma Collection ProQuest Public Health Database Technology Research Database ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Collection Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) Materials Science & Engineering Collection ProQuest Central (Alumni) ProQuest Central UK/Ireland Advanced Technologies & Aerospace Collection Agricultural & Environmental Science Collection ProQuest Central Essentials Biological Science Collection AUTh Library subscriptions: ProQuest Central Technology Collection ProQuest Natural Science Collection Environmental Sciences and Pollution Management ProQuest One Community College ProQuest Materials Science Collection ProQuest Central Engineering Research Database Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student AIDS and Cancer Research Abstracts SciTech Premium Collection (Proquest) (PQ_SDU_P3) ProQuest Health & Medical Complete (Alumni) Materials Science Database Nursing & Allied Health Database (Alumni Edition) Meteorological & Geoastrophysical Abstracts - Academic ProQuest Engineering Collection Biological Sciences Agricultural Science Database Health & Medical Collection (Alumni Edition) PML(ProQuest Medical Library) Algology Mycology and Protozoology Abstracts (Microbiology C) Biological Science Database Engineering Database Nursing & Allied Health Premium Advanced Technologies & Aerospace Database ProQuest Advanced Technologies & Aerospace Collection Biotechnology and BioEngineering Abstracts Environmental Science Database Materials Science Collection Publicly Available Content Database ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China Engineering Collection Environmental Science Collection Genetics Abstracts MEDLINE - Academic PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef Agricultural Science Database Publicly Available Content Database ProQuest Central Student ProQuest Advanced Technologies & Aerospace Collection ProQuest Central Essentials Nucleic Acids Abstracts SciTech Premium Collection ProQuest Central China Environmental Sciences and Pollution Management Health Research Premium Collection Meteorological & Geoastrophysical Abstracts Natural Science Collection Biological Science Collection ProQuest Medical Library (Alumni) Engineering Collection Advanced Technologies & Aerospace Collection Engineering Database Virology and AIDS Abstracts ProQuest Biological Science Collection ProQuest One Academic Eastern Edition Agricultural Science Collection ProQuest Hospital Collection ProQuest Technology Collection Health Research Premium Collection (Alumni) Biological Science Database Ecology Abstracts ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts Environmental Science Collection Entomology Abstracts Nursing & Allied Health Premium ProQuest Health & Medical Complete ProQuest One Academic UKI Edition Environmental Science Database ProQuest Nursing & Allied Health Source (Alumni) Engineering Research Database ProQuest One Academic Meteorological & Geoastrophysical Abstracts - Academic Technology Collection Technology Research Database Materials Science Collection ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest Natural Science Collection ProQuest Pharma Collection ProQuest Central Genetics Abstracts ProQuest Engineering Collection Biotechnology Research Abstracts Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) Agricultural & Environmental Science Collection AIDS and Cancer Research Abstracts Materials Science Database ProQuest Materials Science Collection ProQuest Public Health ProQuest Nursing & Allied Health Source ProQuest SciTech Collection Advanced Technologies & Aerospace Database ProQuest Medical Library Animal Behavior Abstracts Materials Science & Engineering Collection Immunology Abstracts ProQuest Central (Alumni) MEDLINE - Academic |
DatabaseTitleList | MEDLINE Agricultural Science Database |
Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 4 dbid: 8FG name: ProQuest Technology Collection url: https://search.proquest.com/technologycollection1 sourceTypes: Aggregation Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Sciences (General) |
DocumentTitleAlternate | Estrogenic Potential of Flavonoids |
EISSN | 1932-6203 |
Editor | Jeong, Jae-wook |
Editor_xml | – sequence: 1 givenname: Jae-wook surname: Jeong fullname: Jeong, Jae-wook |
EndPage | e74881 |
ExternalDocumentID | 1438700442 oai_doaj_org_article_7807ad48aef44a6e88f4f313bad7d81b 3087142581 A478446689 10_1371_journal_pone_0074881 24098354 |
Genre | Research Support, Non-U.S. Gov't Journal Article |
GeographicLocations | Brazil United States--US |
GeographicLocations_xml | – name: United States--US – name: Brazil |
GroupedDBID | --- 123 29O 2WC 3V. 53G 5VS 7RV 7X2 7X7 7XC 88E 8AO 8C1 8CJ 8FE 8FG 8FH 8FI 8FJ A8Z AAFWJ ABDBF ABIVO ABJCF ABUWG ACGFO ACIHN ACIWK ACPRK ADBBV ADRAZ AEAQA AENEX AFKRA AFRAH AHMBA ALIPV ALMA_UNASSIGNED_HOLDINGS AOIJS APEBS ARAPS ATCPS BAWUL BBNVY BBORY BCNDV BENPR BGLVJ BHPHI BKEYQ BPHCQ BVXVI BWKFM CCPQU CGR CS3 CUY CVF D1I D1J D1K DIK DU5 E3Z EAP EAS EBD ECM EIF EMOBN ESTFP ESX EX3 F5P FPL FYUFA GROUPED_DOAJ GX1 HCIFZ HH5 HMCUK HYE IAO IEA IHR IHW INH INR IOV IPNFZ IPY ISE ISR ITC K6- KB. KQ8 L6V LK5 LK8 M0K M1P M48 M7P M7R M7S M~E NAPCQ NPM O5R O5S OK1 P2P P62 PATMY PDBOC PIMPY PQQKQ PROAC PSQYO PTHSS PV9 PYCSY RIG RNS RPM RZL SV3 TR2 UKHRP WOQ WOW ~02 ~KM AAYXX AFPKN CITATION 7QG 7QL 7QO 7SN 7SS 7T5 7TG 7TM 7U9 7XB 8FD 8FK AZQEC C1K DWQXO FR3 GNUQQ H94 K9. KL. M7N P64 PQEST PQUKI PRINS RC3 7X8 5PM - 02 AAPBV ABPTK ADACO BBAFP KM |
ID | FETCH-LOGICAL-c758t-21d63d24c08c2dd662848bca182134a1b8a7cd27dad8f013a33c2e585d379fbe3 |
IEDL.DBID | RPM |
ISSN | 1932-6203 |
IngestDate | Fri Nov 26 17:53:13 EST 2021 Tue Oct 22 14:58:54 EDT 2024 Tue Sep 17 21:23:41 EDT 2024 Fri Jun 28 06:35:33 EDT 2024 Thu Oct 10 18:14:00 EDT 2024 Thu Feb 22 23:54:20 EST 2024 Fri Feb 02 04:05:29 EST 2024 Thu Aug 01 20:12:56 EDT 2024 Thu Aug 01 19:39:30 EDT 2024 Tue Aug 20 22:11:08 EDT 2024 Fri Aug 23 03:32:48 EDT 2024 Tue Oct 15 23:46:23 EDT 2024 |
IsDoiOpenAccess | true |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 10 |
Language | English |
License | This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. Creative Commons Attribution License |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c758t-21d63d24c08c2dd662848bca182134a1b8a7cd27dad8f013a33c2e585d379fbe3 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Competing Interests: The authors have declared that no competing interests exist. Conceived and designed the experiments: FAR WV EAV. Performed the experiments: FAR APSO MSC. Analyzed the data: FAR. Contributed reagents/materials/analysis tools: WV EAV. Wrote the paper: FAR. |
OpenAccessLink | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3788058/ |
PMID | 24098354 |
PQID | 1438700442 |
PQPubID | 1436336 |
PageCount | e74881 |
ParticipantIDs | plos_journals_1438700442 doaj_primary_oai_doaj_org_article_7807ad48aef44a6e88f4f313bad7d81b pubmedcentral_primary_oai_pubmedcentral_nih_gov_3788058 proquest_miscellaneous_1443415100 proquest_journals_1438700442 gale_infotracmisc_A478446689 gale_infotracacademiconefile_A478446689 gale_incontextgauss_ISR_A478446689 gale_incontextgauss_IOV_A478446689 gale_healthsolutions_A478446689 crossref_primary_10_1371_journal_pone_0074881 pubmed_primary_24098354 |
PublicationCentury | 2000 |
PublicationDate | 2013-10-01 |
PublicationDateYYYYMMDD | 2013-10-01 |
PublicationDate_xml | – month: 10 year: 2013 text: 2013-10-01 day: 01 |
PublicationDecade | 2010 |
PublicationPlace | United States |
PublicationPlace_xml | – name: United States – name: San Francisco – name: San Francisco, USA |
PublicationTitle | PloS one |
PublicationTitleAlternate | PLoS One |
PublicationYear | 2013 |
Publisher | Public Library of Science Public Library of Science (PLoS) |
Publisher_xml | – name: Public Library of Science – name: Public Library of Science (PLoS) |
References | 19654042 - Toxicol In Vitro. 2009 Oct;23(7):1242-8 15290790 - Environ Toxicol Chem. 2004 Mar;23(3):705-11 19141368 - Bioorg Med Chem. 2009 Feb 1;17(3):1109-17 21334935 - J Mol Graph Model. 2011 Apr;29(6):784-94 21432561 - Environ Health Prev Med. 2010 Sep;15(5):319-26 11258977 - Chem Res Toxicol. 2001 Mar;14(3):280-94 16428019 - Phytomedicine. 2006 Feb;13(3):139-45 23523586 - Toxicol Appl Pharmacol. 2013 Jun 1;269(2):176-86 11295156 - Mutat Res. 2001 Apr 18;475(1-2):89-111 10658900 - Chem Biol Interact. 2000 Jan 3;124(1):29-51 7498097 - Environ Health Perspect. 1995 Sep;103(9):844-50 11392124 - Environ Toxicol Chem. 2001 Jun;20(6):1152-8 23376612 - J Steroid Biochem Mol Biol. 2013 Sep;137:165-73 19730910 - Environ Sci Pollut Res Int. 2010 Feb;17(2):250-60 18095219 - Planta Med. 2008 Jan;74(1):25-32 20633926 - Sci Total Environ. 2010 Sep 15;408(20):4451-60 20494649 - Arch Biochem Biophys. 2010 Sep 1;501(1):170-5 10989984 - Breast Cancer Res Treat. 2000 Jul;62(1):35-49 2005797 - Methods Enzymol. 1991;194:373-88 18970573 - Talanta. 2006 Apr 15;69(2):351-8 21397020 - J Steroid Biochem Mol Biol. 2011 Oct;127(1-2):139-46 20227763 - Environ Int. 2010 May;36(4):361-7 20070239 - Antioxid Redox Signal. 2010 Sep 1;13(5):691-719 23524099 - Toxicol In Vitro. 2013 Aug;27(5):1467-75 15568770 - J Nat Prod. 2004 Nov;67(11):1829-32 15893793 - Chemosphere. 2005 Dec;61(11):1710-9 9751507 - Endocrinology. 1998 Oct;139(10):4252-63 9071738 - Steroids. 1997 Mar;62(3):268-303 23462505 - Mol Pharmacol. 2013 May;83(5):1066-77 ref13 LR Ferguson (ref19) 2001; 475 S Liu (ref24) 2013; 85(5) H Fang (ref25) 2001; 14 T Ogawa (ref3) 2009; 17 ref10 TN Noguerol (ref8) 2006; 69(2) ref2 W De-Eknamkul (ref31) 2011; 29 B Kuch (ref16) 2010; 17 R Brix (ref1) 2010; 36 SM Oh (ref23) 2009; 23 DX Hou (ref28) 2010; 13 GM Anstead (ref26) 1997; 62 JC Schneider (ref11) 1991; 194 T Schiliró (ref15) 2011; 127 R Céspedes (ref7) 2005; 61 C Watanabe (ref30) 2010; 15 RS Zand (ref18) 2000; 62 M Villalobos (ref12) 1995; 103 GJ Wanda (ref21) 2006; 13 N Garcia-Reyero (ref6) 2001; 20 I Duarte Silva (ref29) 2000; 124 N Garcia-Reyero (ref9) 2004; 23 P Tarnow (ref14) 2013; 27(5) SY Choi (ref17) 2008; 74 GG Kuiper (ref20) 1998; 139 A De Naeyer (ref4) 2004; 67(11) C Vanparys (ref22) 2010; 408 S Sharan (ref5) 2013; 269(2) HA Ward (ref27) 2010; 501 |
References_xml | – volume: 14 start-page: 280 year: 2001 ident: ref25 article-title: Structure-activity relationships for a large diverse set of natural, synthetic, and environmental estrogens publication-title: Chem Res Toxicol doi: 10.1021/tx000208y contributor: fullname: H Fang – volume: 13 start-page: 139 year: 2006 ident: ref21 article-title: Estrogenic properties of isoflavones derived from Millettia griffoniana. publication-title: Phytomed doi: 10.1016/j.phymed.2005.06.003 contributor: fullname: GJ Wanda – volume: 124 start-page: 29 year: 2000 ident: ref29 article-title: Chemical features of flavonols affecting their genotoxicity. Potential implications in their use as therapeutical agents publication-title: Chem Biol Interact doi: 10.1016/S0009-2797(99)00139-8 contributor: fullname: I Duarte Silva – volume: 17 start-page: 250 year: 2010 ident: ref16 article-title: Effect-related monitoring: estrogen-like substances in groundwater publication-title: Environ Sci Poll Res Int doi: 10.1007/s11356-009-0234-1 contributor: fullname: B Kuch – volume: 13 start-page: 691 year: 2010 ident: ref28 article-title: Flavonoids as protein kinase inhibitors for cancer chemoprevention: direct binding and molecular modeling publication-title: Antiox Redox Signal doi: 10.1089/ars.2009.2816 contributor: fullname: DX Hou – volume: 74 start-page: 25 year: 2008 ident: ref17 article-title: Estrogenic activities of isoflavones and flavones and their structure-activity relationships publication-title: Planta Med doi: 10.1055/s-2007-993760 contributor: fullname: SY Choi – volume: 15 start-page: 319 year: 2010 ident: ref30 article-title: DNA damage and estrogenic activity induced by the environmental pollutant 2-nitrotoluene and its metabolite publication-title: Environ Health Prev Med doi: 10.1007/s12199-010-0146-1 contributor: fullname: C Watanabe – volume: 61 start-page: 1710 year: 2005 ident: ref7 article-title: Distribution of endocrine disruptors in the Llobregat River basin (Catalonia, NE Spain) publication-title: Chemosphere doi: 10.1016/j.chemosphere.2005.03.082 contributor: fullname: R Céspedes – volume: 103 start-page: 844 year: 1995 ident: ref12 article-title: The E-screen assay: a comparison of different MCF-7 cell stocks publication-title: Environ Health Perspect doi: 10.1289/ehp.95103844 contributor: fullname: M Villalobos – volume: 17 start-page: 1109 year: 2009 ident: ref3 article-title: Synthesis and biological evaluation of p-carborane bisphenols and their derivatives: structure-activity relationship for estrogenic activity publication-title: Bioorg Med Chem doi: 10.1016/j.bmc.2008.12.044 contributor: fullname: T Ogawa – volume: 23 start-page: 705 year: 2004 ident: ref9 article-title: Estrogenic potential of halogenated derivatives of nonylphenol ethoxylates and carboxylates publication-title: Environ Toxicol Chem doi: 10.1897/03-141 contributor: fullname: N Garcia-Reyero – ident: ref13 – volume: 85(5) start-page: 1066 year: 2013 ident: ref24 article-title: Cooperative activation of gene expression by agonists and antagonists mediated by estrogen receptor heteroligand dimer complexes publication-title: Mol Pharmacol doi: 10.1124/mol.112.084228 contributor: fullname: S Liu – volume: 36 start-page: 361 year: 2010 ident: ref1 article-title: Evaluation of the suitability of recombinant yeast-based estrogenicity assays as a pre-screening tool in environmental samples publication-title: Environ Int doi: 10.1016/j.envint.2010.02.004 contributor: fullname: R Brix – volume: 194 start-page: 373 year: 1991 ident: ref11 article-title: Vectors for expression of cloned genes in yeast: Regulation, overproduction, and underproduction publication-title: Meth Enzymol doi: 10.1016/0076-6879(91)94028-B contributor: fullname: JC Schneider – volume: 67(11) start-page: 1829 year: 2004 ident: ref4 article-title: Estrogenic and anticarcinogenic properties of kurarinone, a lavandulyl flavanone from the roots of Sophora flavescens publication-title: J Nat Prod doi: 10.1021/np040069a contributor: fullname: A De Naeyer – volume: 27(5) start-page: 1467 year: 2013 ident: ref14 article-title: Effects of triclocarban on the transcription of estrogen, androgen and aryl hydrocarbon receptor responsive genes in human breast cancer cells publication-title: Toxicol In Vitro doi: 10.1016/j.tiv.2013.03.003 contributor: fullname: P Tarnow – ident: ref2 doi: 10.1016/j.jsbmb.2013.01.003 – volume: 20 start-page: 1152 year: 2001 ident: ref6 article-title: Monitoring of endocrine disruptors in surface waters by the yeast recombinant assay publication-title: Environ Toxicol Chem doi: 10.1002/etc.5620200603 contributor: fullname: N Garcia-Reyero – volume: 501 start-page: 170 year: 2010 ident: ref27 article-title: Phytoestrogen consumption and association with breast, prostate and colorectal cancer in EPIC Norfolk publication-title: Arch Biochem Bioph doi: 10.1016/j.abb.2010.05.018 contributor: fullname: HA Ward – volume: 69(2) start-page: 351 year: 2006 ident: ref8 article-title: Detection of hormone receptor ligands in yeast by fluorogenic methods publication-title: Talanta doi: 10.1016/j.talanta.2005.09.044 contributor: fullname: TN Noguerol – volume: 62 start-page: 35 year: 2000 ident: ref18 article-title: Steroid hormone activity of flavonoids and related compounds publication-title: Breast Cancer Res Treat doi: 10.1023/A:1006422302173 contributor: fullname: RS Zand – volume: 269(2) start-page: 176 year: 2013 ident: ref5 article-title: Effects of low dose treatment of tributyltin on the regulation of estrogen receptor functions in MCF-7 cells. Toxicol Appl Pharmacol contributor: fullname: S Sharan – volume: 139 start-page: 4252 year: 1998 ident: ref20 article-title: Interaction of estrogenic chemicals and phytoestrogens with estrogen receptor beta publication-title: Endocrinol doi: 10.1210/endo.139.10.6216 contributor: fullname: GG Kuiper – volume: 127 start-page: 139 year: 2011 ident: ref15 article-title: Endocrine disrupting activity in fruits and vegetables evaluated with the E-screen assay in relation to pesticide residues publication-title: J Steroid Biochem Mol Biol doi: 10.1016/j.jsbmb.2011.03.002 contributor: fullname: T Schiliró – volume: 29 start-page: 784 year: 2011 ident: ref31 article-title: QSAR study of natural estrogen-like isoflavonoids and diphenolics from Thai medicinal plants publication-title: J Mol Graph Model doi: 10.1016/j.jmgm.2011.01.001 contributor: fullname: W De-Eknamkul – volume: 23 start-page: 1242 year: 2009 ident: ref23 article-title: In vitro estrogenic and antiestrogenic potential of chlorostyrenes publication-title: Toxicol In Vitro doi: 10.1016/j.tiv.2009.07.032 contributor: fullname: SM Oh – volume: 62 start-page: 268 year: 1997 ident: ref26 article-title: The estradiol pharmacophore: ligand structure-estrogen receptor binding affinity relationships and a model for the receptor binding site publication-title: Steroids doi: 10.1016/S0039-128X(96)00242-5 contributor: fullname: GM Anstead – ident: ref10 – volume: 475 start-page: 89 year: 2001 ident: ref19 article-title: Role of plant polyphenols in genomic stability publication-title: Mutat Res doi: 10.1016/S0027-5107(01)00073-2 contributor: fullname: LR Ferguson – volume: 408 start-page: 4451 year: 2010 ident: ref22 article-title: Performance of the flow cytometric E-screen assay in screening estrogenicity of pure compounds and environmental samples publication-title: Sci Total Environ doi: 10.1016/j.scitotenv.2010.06.049 contributor: fullname: C Vanparys |
SSID | ssj0053866 |
Score | 2.4460032 |
Snippet | Phytoestrogens are of interest because of their reported beneficial effects on many human maladies including cancer, neurodegeneration, cardiovascular disease... |
SourceID | plos doaj pubmedcentral proquest gale crossref pubmed |
SourceType | Open Website Open Access Repository Aggregation Database Index Database |
StartPage | e74881 |
SubjectTerms | 17β-Estradiol Assaying Breast cancer Cancer Cardiovascular diseases Cell cycle Cell growth Cell proliferation Cell Proliferation - drug effects Cell Survival - drug effects Chemicals Diabetes mellitus DNA, Recombinant - genetics Estradiol Estrogen receptors Estrogenic activity Estrogens Evaluation Flavonoids Flavonoids - metabolism Flavonoids - pharmacology Gene expression Hormone replacement therapy Humans Hydroxyl groups Hydroxylation Isoflavones Kaempferol Kinases Ligands MCF-7 Cells Menopause Neurodegeneration Pharmaceutical sciences Phytoestrogens Phytoestrogens - metabolism Phytoestrogens - pharmacology Plasmids Quercetin Sex hormones Transcription activation Xenoestrogens Yeast Yeasts - cytology Yeasts - genetics |
SummonAdditionalLinks | – databaseName: DOAJ Directory of Open Access Journals dbid: DOA link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9QwELbQnrggyquBAgYhAYewm9hrO8e26qogFSTKot4sP-J2pZJEzS6o_54ZJxs1qBIcuMaT18w39kwyno-QN7ZwcY9Mmst5SHnmfKqYM6l3TlnGnLCRP-Xkszhe8k9n87MbVF9YE9a1B-4UN5VqJo3nypSBcyNKpQIPLGPWeOkh5oqzbzbfJlPdHAxeLES_UY7JbNrb5UNTVyU2ywbUZqOFKPbrH2blSXNZt7eFnH9WTt5Yihb3yb0-hqT73bPvkDtl9YDs9F7a0nd9K-n3D8mvo6GZN60DhZtc1YCYlaNNvcY6IbgMHA-X5mdd1SvfUqyDP6eGYqL8w8YyGXqN_D60jWwS1FSenhwu5PRgeUrxsz9tkPgnlB2UKATj5voRWS6Ovh0epz3VQuogYVineeYF8zl3M-Vy74WAVUtZZyD7yBg3mVVGOp9Lb7wKEDUaMGNeQqrhmSyCLdljMqlAubuE5l4E5QsBSrKcz8Dk3gikNHbMhiIUCUm3etdN11FDx99qEjKRToEa7aR7OyXkAI0zyGI_7HgAUKJ7lOi_oSQhL9G0uttcOni13udS4R9tBY_1OkpgT4wKi27OzaZt9ccv3_9B6PTrSOhtLxRqsIwz_UYHeCfstTWS3BtJgme70fAuAnGrlVYjVT3SEfAcztyC8_bhV8MwXhQL6aqy3qAMh7gFJuJZQp50WB40C8Fdgd8BEyJHKB-pfjxSrS5iS3JkJZjN1dP_Yatn5G6OnCOxYnKPTNZXm_I5RH5r-yI6-W-7t1nf priority: 102 providerName: Directory of Open Access Journals – databaseName: ProQuest Public Health Database dbid: 8C1 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV3fb9MwELagvPCCGL9WNsAgJOAhtIld23lCW9VqIA0mStF4ihw7HpVK0jUtiP-eO8cNBE2I1_gapffd2Xf2-T5Cnuep8XdkokSOXMRjYyPFjI6sMSpnzIjc86ecvhcnc_7ufHQeNtzqUFa5mxP9RG0rg3vkA6TpxlbsPHmzuoyQNQpPVwOFxnVyIwbDROoGNW5LPMCXhQjX5ZiMBwGd16uqLLBlNthu3FmOfNf-dm7urZZVfVXg-Xf95B8L0vQ2uRUiSXrUQL9HrhXlHbIXfLWmL0ND6Vd3yY9J29KbVo5O6s26ArtZGHpWbbBaCF4Dz6dL_b0qq4WtqS8koJpicvot98Uy9Auy_NCZ55SgurT0dDyVg-P5jI6L5ZKeIf2PKxqDooC6_nmPzKeTT-OTKBAuRAbShk2UxFYwm3AzVCaxVghYu1RuNOQgMeM6zpWWxibSaqscxI4awEwKSDgsk6nLC3af9EpQ7j6hiRVO2VSAknLOhwC81QKJjQ3LXerSPol2es9WTV-NzB-uSchHGgVmiFMWcOqTYwSnlcWu2P5Btb7IgpNlUg2ltlzpwnGuRaGU447FLNdWWojP--QJQps1V0xb386OuFR4rq3gs555CeyMUWLpzYXe1nX29sPn_xCafewIvQhCrgJkjA7XHeA_YcetjuRhRxL823SG99EQd1qps9-eAL_cGefVw0_bYXwpltOVRbVFGQ7RC0zHwz550Nhyq1kI8VLcDewT2bHyjuq7I-Xiq29MjtwEw5F6-O_POiA3E-QU8RWRh6S3WW-LRxDZbfLH3n1_AcdOTuc priority: 102 providerName: ProQuest – databaseName: Scholars Portal Journals: Open Access dbid: M48 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV3db9MwELem8sILYnytY4BBSMBDRj5c23lAaKtaDaTCRCkaT5E_4lGpJKVpgf333DlpRFAn8Rqfo-Tud_FdfL4fIc91avwZmSAWAxewyNhAJkYF1hipk8Rw7flTJh_42Yy9vxhc7JEtZ2ujwGpnaod8UrPV4vj3j6u34PBvPGuDiLaTjpdlkWMrbMAk5EM3YpYwxPyEtfsK4N2cNwforpvZWaB8H__2a91bLspqVyj6b0XlX0vU-Da51cSW9KQGwz7Zy4s7ZL_x3oq-bFpMv7pLfo3aJt-0dHRUrVclIGlu6Hm5xvohuA1cHy_Uz7Io57aivrSAKorp6nfty2foV-T9oVPPMkFVYelkOBavT2dTOswXC3qOhEAuryFGAQfq6h6ZjUefh2dBQ8EQGEgk1kEcWZ7YmJlQmthazmE1k9ooyEqihKlISyWMjYVVVjqIJhWYN84hBbGJSJ3Ok_ukV4ByDwiNLXfSphyUpBkLAQpWcaQ6Nol2qUv7JNjqPVvWnTYyv90mIEOpFZihnbLGTn1yisZpZbFPtr9Qri6zxu0yIUOhLJMqd4wpnkvpmEuiRCsrLETsffIETZvVh05bb89OmJC40y3hsZ55CeyVUWAxzqXaVFX27uOX_xCafuoIvWiEXAmWMao5AAHvhD24OpJHHUnweNMZPkAgbrVSZUhhjzQFLIaZW3DuHn7aDuNNscCuyMsNyoC3QPQXhn3yoMZyq1kI-lL8P9gnooPyjuq7I8X8m29VjmwF4UAeXv_ED8nNGBlGfH3kEemtV5v8EcR5a_3Yu-4fVH9TQA priority: 102 providerName: Scholars Portal |
Title | Evaluation of estrogenic potential of flavonoids using a recombinant yeast strain and MCF7/BUS cell proliferation assay |
URI | https://www.ncbi.nlm.nih.gov/pubmed/24098354 https://www.proquest.com/docview/1438700442 https://search.proquest.com/docview/1443415100 https://pubmed.ncbi.nlm.nih.gov/PMC3788058 https://doaj.org/article/7807ad48aef44a6e88f4f313bad7d81b http://dx.doi.org/10.1371/journal.pone.0074881 |
Volume | 8 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lj9MwELZ2y4ULYnltYSkGIQGHbJvYjZ3jtmpZkLpUW4rKKfIj2a3UJlXTgvj3zDhJRdAeEBcf4kmUzMOecWa-IeStjoyrkfEC0U897hvrSWaUZ42RmjETatc_ZXIVXs7550V_cUT6dS2MS9o3enmerdbn2fLW5VZu1qZb54l1p5MhYqD3-rJ7TI4FY3WIXi6_YMBhWNXIMeF3K5Gcb_IsQZxsUFjsDgMbWYRnHo3tyKH2H9bm1maVF3c5nn_nT_6xIY0fkgeVJ0kvyjc-IUdJ9oicVLZa0PcVoPSHx-Tn6ADpTfOUjordNge9WRo6zXeYLQSPgevjlfqRZ_nSFtQlElBFMThda5csQ79jlx86cz0lqMosnQzHojuYz-gwWa3oFNv_pEmpUBSkrn49IfPx6Ovw0qsaLngGwoadF_g2ZDbgpidNYG0Ywt4ltVEQg_iMK19LJYwNhFVWpuA7KhBmkEDAYZmIUp2wp6SVAZ9PCQ1smEobhcAkzXkPBG9ViI2NDdNplEZt4tV8jzclrkbsfq4JiEdKBsYosrgSWZsMUDgHWkTFdhfy7U1c6UYsZE8oy6VKUs5VmEiZ8pT5TCsrLPjnbfIKRRuXJaYH244vuJD4X1vCa71xFIiMkWHqzY3aF0X86cu3fyCaXTeI3lVEaQ6SMaoqd4BvQsStBuVZgxLs2zSmT1ERa64UMTasx6YEPIA7a-W8e_r1YRofiul0WZLvkYaD9wLLca9NnpW6fOBsbRltIhpa3mB9cwbs1AGTV3b5_L_vfEHuB9huxCVLnpHWbrtPXoLTt9MdMPWFgFEOfRzHHzvk3mB0Nb3uuGMUGCdcdtxS8BsjP15A |
link.rule.ids | 230,315,733,786,790,870,891,2115,2236,12083,12250,12792,21416,24346,27955,27956,31752,31753,33299,33300,33406,33407,33777,33778,43343,43612,43633,43838,53825,53827,74100,74369,74390,74657 |
linkProvider | National Library of Medicine |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV3Nb9MwFLegO8AFMb5WGMwgJOAQ2iRu7JzQWrXqYC3VuqFxihw7HpVKUpoWxH_Pe44bCJoQ1_g1St-Xn-3n34-Ql2ms7B0ZL-A94zFfaU-ESnpaKZGGoYpSy58ymUbjC_b-snfpNtxK11a5y4k2UetC4R55B2m6EYqdBe9W3zxkjcLTVUehcZPsIeSmaJG9_nA6O9vlYojmKHIX5kLud5x93q6KPEPQbPBevzEhWdz-Oju3VsuivK70_LuD8o8paXSX3HG1JD2ujL9PbmT5PbLvorWkrx2k9Jv75MewBvWmhaHDcrMuwHMWis6KDfYLwWvg-Wgpvxd5sdAlta0EVFJcnn5NbbsM_Yw8P3RuWSWozDWdDEa807-Y00G2XNIZEgCZrHIpCnaXPx-Qi9HwfDD2HOWCp2DhsPECX0ehDpjqChVoHUUwe4lUSViF-CGTfiokVzrgWmphoHqUYM4ggyWHDnls0ix8SFo5KPeA0EBHRug4AiWljHXB9FpGSG2swtTEJm4Tb6f3ZFUhayT2eI3DiqRSYIJ2Spyd2qSPxqllERfbPijWV4kLs4SLLpeaCZkZxmSUCWGYCf0wlZprqNDb5AhNm1SXTOvoTo4ZF3iyLeCzXlgJxMbIsfnmSm7LMjn5-Ok_hOZnDaFXTsgUYBkl3YUH-E-IudWQPGxIQoSrxvABOuJOK2XyOxbglzvnvH74eT2ML8WGujwrtijDoH6BhNxtk0eVL9eahSIvxv3ANuENL2-ovjmSL75YaHJkJ-j2xON_f9YRuTU-n5wmpyfTD0_I7QAZRmx_5CFpbdbb7CnUeZv0mQvmX9cCU3s |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV3fb9MwELagSIgXxPi1wmAGIQEPoU3i2s4T2kqrDdioKJ3GU-TY8ahUktK0IP577hw3EDQhXuNLlNx9Z9_F5_sIeZYl2p2RCSIxsAELtQlkrFVgtJZZHGueOf6Uk1N-NGNvzwfnvv6p8mWV2znRTdSm1PiPvIc03diKnUU968siJm_Gr5ffAmSQwp1WT6dxlVyDVZEjwuWwKfcAv-bcH52LRdjzlnq1LIsc22cDjsPW0uQ6-DfzdGe5KKvLgtC_ayn_WJzGt8hNH1XSgxoGO-RKXtwmO95vK_rCN5d-eYf8GDXtvWlp6ahar0rA0FzTSbnGyiF4DFwfL9T3sijnpqKuqIAqionq18wVztDPyPhDp45fgqrC0JPhWPQOZ1M6zBcLOkEqIJvX4KKAAPXzLpmNR5-GR4EnXwg0pBDrIAoNj03EdF_qyBjOYR2TmVaQj4QxU2EmldAmEkYZaSGOVGDYKIfkw8QisVke3yOdApS7S2hkuJUm4aCkjLE-gMAojiTHOs5sYpMuCbZ6T5d1j43UbbQJyE1qBaZop9TbqUsO0TiNLHbIdhfK1UXqHS4Vsi-UYVLlljHFcykts3EYZ8oIA7F6l-yjadP6uGnj5-kBExL3uCW81lMngV0yCsTbhdpUVXr84ew_hKYfW0LPvZAtwTJa-aMP8E3YfasludeSBF_XreFdBOJWK1X62yvgzi04Lx9-0gzjQ7G0rsjLDcowiGRgau53yf0ay41mIdxL8M9gl4gWyluqb48U8y-uSTnyFPQH8sG_X2ufXAcvTt8fn757SG5ESDXiCiX3SGe92uSPIOBbZ4-dJ_8CzyhWFQ |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Evaluation+of+Estrogenic+Potential+of+Flavonoids+Using+a+Recombinant+Yeast+Strain+and+MCF7%2FBUS+Cell+Proliferation+Assay&rft.jtitle=PloS+one&rft.au=Resende%2C+Fl%C3%A1via&rft.au=Ana+Paula+S+de+Oliveira&rft.au=de+Camargo%2C+Mariana&rft.au=Wagner+Vilegas&rft.date=2013-10-01&rft.pub=Public+Library+of+Science&rft.eissn=1932-6203&rft.volume=8&rft.issue=10&rft_id=info:doi/10.1371%2Fjournal.pone.0074881&rft.externalDocID=1438700442 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1932-6203&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1932-6203&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1932-6203&client=summon |