Evaluation of Disulfide Bond Position to Enhance the Thermal Stability of a Highly Stable Single Domain Antibody - Zabetakis, Dan; Olson, Mark A.; Anderson, George P.; Legler, Patricia M.; Goldman, Ellen R. (PloS one, 2014)
Keywords: Affinity; Analysis; Analytical chemistry; Animals; Antibodies; Antibody Affinity; Bioinformatics; Biology; Biology and Life Sciences; Calorimetry, Differential Scanning; Camelids, New World - immunology; Chemical bonds; Chromatography; Circular Dichroism; Crystal structure; Cysteine - genetics; Denaturation; Disulfide bonds; Disulfides - chemistry; Engineering; Enterotoxin B; Enterotoxins - metabolism; Immunoassay; Immunoglobulins; Immunology; Laboratories; Medicine and Health Sciences; Melting; Melting point; Melting points; Models, Molecular; Mutagenesis; Mutation; Nanobodies; Phase transitions; Physical Sciences; Protein denaturation; Protein expression; Protein folding; Protein Refolding; Protein Stability; Simulation; Single-Domain Antibodies - chemistry; Single-Domain Antibodies - genetics; Single-Domain Antibodies - metabolism; Solvents; Stability analysis; Staphylococcal enterotoxin A; Staphylococcal enterotoxin B; Temperature effects; Thermal stability; Transition Temperature