The Macrophage-Specific Promoter mfap4 Allows Live, Long-Term Analysis of Macrophage Behavior during Mycobacterial Infection in Zebrafish

• Published in: PloS one Vol. 10; no. 10; p. e0138949
• Main Authors: Walton, Eric M, Cronan, Mark R, Beerman, Rebecca W, Tobin, David M
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 07.10.2015; Public Library of Science (PLoS)
• Subjects: Animals; Animals, Genetically Modified - genetics; Animals, Genetically Modified - microbiology; Artificial chromosomes; Bacterial infections; Cell Lineage - genetics; Cloning; Danio rerio; Deoxyribonucleic acid; Disease Models, Animal; DNA; Gene expression; Genetic engineering; Genetics; Genomes; Health aspects; Host-Pathogen Interactions - genetics; Immune system; Immunity, Innate - genetics; Immunology; Infection; Infections; Labeling; Labelling; Larva - genetics; Larva - microbiology; Macrophages; Macrophages - microbiology; Mammals; Microorganisms; Mycobacterium Infections - genetics; Mycobacterium Infections - microbiology; Mycobacterium marinum; Mycobacterium marinum - pathogenicity; Neutrophils; Pathogenesis; Promoter Regions, Genetic - genetics; Proteins; Rodents; Salmonella; Salmonella typhimurium - pathogenicity; Transcription; Transgenes - genetics; Zebrafish; Zebrafish - genetics; Zebrafish - microbiology; Zebrafish Proteins - genetics; North Carolina; United States > US

Transgenic labeling of innate immune cell lineages within the larval zebrafish allows for real-time, in vivo analyses of microbial pathogenesis within a vertebrate host. To date, labeling of zebrafish macrophages has been relatively limited, with the most specific expression coming from the mpeg1 pr...