Development of an Efficient Recombinant Mosquito Densovirus-Mediated RNA Interference System and Its Preliminary Application in Mosquito Control

• Published in: PloS one Vol. 6; no. 6; p. e21329
• Main Authors: Gu, Jinbao, Liu, Min, Deng, Yuhua, Peng, Hongjuan, Chen, Xiaoguang
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 16.06.2011; Public Library of Science (PLoS)
• Subjects: Adenosine triphosphatase; Aedes aegypti; Aedes albopictus; Animals; Anopheles gambiae; Aquatic insects; ATPases; Base Sequence; Bioassays; Biology; Caenorhabditis elegans; Cloning; Crop diseases; Culicidae; Culicidae - growth & development; Culicidae - virology; Densovirus - genetics; Disease prevention; DNA Primers; Gene expression; Genes; Genetic engineering; Genomes; Genomics; H+-transporting ATPase; Higher education; Infection; Infections; Infectious diseases; Insect control; Insects; Interference; Laboratories; Larvae; Medicine; Mosquito Control; Mosquitoes; Nucleic acids; Plasmids; Proteins; Public health; Recombination, Genetic; Reverse Transcriptase Polymerase Chain Reaction; Ribonucleic acid; RNA; RNA Interference; RNA-mediated interference; Transfection; Trends; Tribolium; Vectors; Viruses; United States > US; China

The Aedes aegypti densovirus (AeDNV) has potential as a delivery vector for foreign nucleic acids into mosquito cells. In this study, we investigated the ability of plasmids containing recombinant viral transducing genome to induce RNA interference (RNAi) effects in C6/C36 cells. We then evaluated t...