Osteoblasts and bone marrow mesenchymal stromal cells control hematopoietic stem cell migration and proliferation in 3D in vitro model

• Published in: PloS one Vol. 5; no. 2; p. e9093
• Main Authors: de Barros, Ana Paula D N, Takiya, Christina M, Garzoni, Luciana R, Leal-Ferreira, Mona Lisa, Dutra, Hélio S, Chiarini, Luciana B, Meirelles, Maria Nazareth, Borojevic, Radovan, Rossi, Maria Isabel D
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 08.02.2010; Public Library of Science (PLoS)
• Subjects: Active control; Adult; Analysis; Animals; Antigens, CD34 - analysis; Arthritis; Biocompatibility; Biomedical materials; Bone marrow; Bone Marrow Cells - cytology; Bone Marrow Cells - metabolism; CD34 antigen; Cell Biology; Cell Biology/Cell Adhesion; Cell Biology/Cell Growth and Division; Cell Culture Techniques; Cell Line; Cell migration; Cell Movement; Cell Proliferation; Cells (biology); Cells, Cultured; Chemokine CXCL12 - genetics; Chemokines; Coculture Techniques; Collagen; Colony-forming cells; Cord blood; CXCL12 protein; Diabetes; Extracellular matrix; Fetal Blood - cytology; Fibroblasts; Fibronectin; Fibronectins; Flow Cytometry; Gene expression; Gene Expression Profiling; Growth factors; Hematology/Hematopoiesis; Hematopoietic stem cells; Hematopoietic Stem Cells - cytology; Hematopoietic Stem Cells - metabolism; Humans; Hypoxia; Infant, Newborn; Laminin; Mesenchymal stem cells; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - metabolism; Mesenchyme; Microscopy; Microscopy, Electron; mRNA; Osteoblasts; Osteoblasts - cytology; Osteoblasts - metabolism; Osteopontin; Progenitor cells; Proteins; Reverse Transcriptase Polymerase Chain Reaction; Rodents; Self-assembly; Spheroids; Spheroids, Cellular - cytology; Spheroids, Cellular - metabolism; Spheroids, Cellular - ultrastructure; Stem cells; Stromal cells; Stromal Cells - cytology; Stromal Cells - metabolism; Three dimensional models; Vascular endothelial growth factor; Wnt protein; Brazil; Rio de Janeiro Brazil

Migration, proliferation, and differentiation of hematopoietic stem cells (HSCs) are dependent upon a complex three-dimensional (3D) bone marrow microenvironment. Although osteoblasts control the HSC pool, the subendosteal niche is complex and its cellular composition and the role of each cell popul...