Functional Mutation of Multiple Solvent-Exposed Loops in the Ecballium elaterium Trypsin Inhibitor-II Cystine Knot Miniprotein

• Published in: PloS one Vol. 6; no. 2; p. e16112
• Main Authors: Kimura, Richard H., Jones, Douglas S., Jiang, Lei, Miao, Zheng, Cheng, Zhen, Cochran, Jennifer R.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 18.02.2011; Public Library of Science (PLoS)
• Subjects: Adhesive bonding; Amino Acid Sequence; Amino acids; Animals; Antigenic determinants; Binding; Bioengineering; Biology; Cancer; Cell adhesion; Cell Adhesion - drug effects; Chemical bonds; Cucurbitaceae - chemistry; Cysteine; Cystine; Cystine Knot Motifs - genetics; Cystine Knot Motifs - physiology; Disulfide bonds; Dosage; Ecballium elaterium; Engineering; Epidermal growth factor; Epitopes; Extracellular matrix; Female; Genetic aspects; Humans; Integrins; K562 Cells; Kidneys; Libraries; Matrix protein; Medical screening; Mice; Mice, Nude; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Mutation - physiology; Peptide Fragments - chemical synthesis; Peptide Fragments - chemistry; Peptide Fragments - genetics; Peptide Fragments - pharmacology; Peptides; Positron emission; Positron emission tomography; Protein Binding; Protein Engineering - methods; Protein Folding - drug effects; Protein Structure, Tertiary - genetics; Proteins; Receptors; Sequence Homology, Amino Acid; Solvents; Solvents - pharmacology; Stability; Trypsin; Trypsin inhibitors; Trypsin Inhibitors - chemistry; Trypsin Inhibitors - genetics; Trypsin Inhibitors - isolation & purification; Trypsin Inhibitors - metabolism; Tumor Cells, Cultured; Tumors; Vitronectin; Xenograft Model Antitumor Assays; Xenografts; Yeast; United States > US; California

The Ecballium elaterium trypsin inhibitor (EETI-II), a 28-amino acid member of the knottin family of peptides, contains three interwoven disulfide bonds that form multiple solvent-exposed loops. Previously, the trypsin binding loop of EETI-II has been engineered to confer binding to several alternat...