Functional Mutation of Multiple Solvent-Exposed Loops in the Ecballium elaterium Trypsin Inhibitor-II Cystine Knot Miniprotein - Kimura, Richard H.; Jones, Douglas S.; Jiang, Lei; Miao, Zheng; Cheng, Zhen; Cochran, Jennifer R. (PloS one, 2011)
Keywords: Adhesive bonding; Amino Acid Sequence; Amino acids; Animals; Antigenic determinants; Binding; Bioengineering; Biology; Cancer; Cell adhesion; Cell Adhesion - drug effects; Chemical bonds; Cucurbitaceae - chemistry; Cysteine; Cystine; Cystine Knot Motifs - genetics; Cystine Knot Motifs - physiology; Disulfide bonds; Dosage; Ecballium elaterium; Engineering; Epidermal growth factor; Epitopes; Extracellular matrix; Female; Genetic aspects; Humans; Integrins; K562 Cells; Kidneys; Libraries; Matrix protein; Medical screening; Mice; Mice, Nude; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Mutation - physiology; Peptide Fragments - chemical synthesis; Peptide Fragments - chemistry; Peptide Fragments - genetics; Peptide Fragments - pharmacology; Peptides; Positron emission; Positron emission tomography; Protein Binding; Protein Engineering - methods; Protein Folding - drug effects; Protein Structure, Tertiary - genetics; Proteins; Receptors; Sequence Homology, Amino Acid; Solvents; Solvents - pharmacology; Stability; Trypsin; Trypsin inhibitors; Trypsin Inhibitors - chemistry; Trypsin Inhibitors - genetics; Trypsin Inhibitors - isolation & purification; Trypsin Inhibitors - metabolism; Tumor Cells, Cultured; Tumors; Vitronectin; Xenograft Model Antitumor Assays; Xenografts; Yeast; United States--US; California