Precise spatiotemporal control of optogenetic activation using an acousto-optic device

• Published in: PloS one Vol. 6; no. 12; p. e28468
• Main Authors: Wang, Kaiyu, Liu, Yafeng, Li, Yiding, Guo, Yanmeng, Song, Peipei, Zhang, Xiaohui, Zeng, Shaoqun, Wang, Zuoren
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 09.12.2011; Public Library of Science (PLoS)
• Subjects: Acoustics - instrumentation; Action Potentials - physiology; Action Potentials - radiation effects; Activation; Adenoviruses; Animals; Animals, Genetically Modified; Biology; Brain; Brain slice preparation; Caenorhabditis elegans; Cortex; Deactivation; Drosophila; Drosophila melanogaster - physiology; Drosophila melanogaster - radiation effects; Fruit flies; Gene expression; HEK293 Cells; High resolution; Humans; Inactivation; Laboratories; Lasers; Light; Mice; Microscopy; Nerve Net - physiology; Neural circuitry; Neural networks; Neurons; Neurosciences; Optical Devices; Optics; Photic Stimulation; Physics; Proteins; Questioning; Rats; Rats, Sprague-Dawley; Science; Spinal cord; Stimulation; Synapses - physiology; Synapses - radiation effects; Time Factors; China

Light activation and inactivation of neurons by optogenetic techniques has emerged as an important tool for studying neural circuit function. To achieve a high resolution, new methods are being developed to selectively manipulate the activity of individual neurons. Here, we report that the combinati...