Different Oxidative Stress Response in Keratinocytes and Fibroblasts of Reconstructed Skin Exposed to Non Extreme Daily-Ultraviolet Radiation

• Published in: PloS one Vol. 5; no. 8; p. e12059
• Main Authors: Marionnet, Claire, Pierrard, Cécile, Lejeune, François, Sok, Juliette, Thomas, Marie, Bernerd, Françoise
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 10.08.2010; Public Library of Science (PLoS)
• Subjects: Analysis; Antioxidants; Biological effects; Biomarkers - metabolism; Biotechnology/Bioengineering; Cell Biology/Gene Expression; Cell Differentiation - radiation effects; Computer simulation; Deoxyribonucleic acid; Dermatologic Surgical Procedures; Dermatology; Dermis - cytology; Dermis - radiation effects; DNA; Epidermis - cytology; Epidermis - radiation effects; Exposure; Extreme ultraviolet radiation; Fibroblasts; Fibroblasts - cytology; Fibroblasts - metabolism; Fibroblasts - radiation effects; Gene expression; Gene Expression Profiling; Genomes; Histology; Humans; Irradiance; Keratinocytes; Keratinocytes - cytology; Keratinocytes - metabolism; Keratinocytes - radiation effects; Markers; Mitochondrial DNA; Modulation; Morphogenesis; Morphology; mRNA; Oxidative stress; Oxidative Stress - radiation effects; Physiological aspects; Physiology; Proteins; Pseudomonas aeruginosa; Radiation Dosage; Reconstructive Surgical Procedures; RNA; RNA, Messenger - genetics; RNA, Messenger - metabolism; Simulators; Skin; Skin - cytology; Skin - radiation effects; Solar simulators; Studies; Sun; Three dimensional models; Ultraviolet radiation; Ultraviolet Rays
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0012059

Experiments characterizing the biological effects of sun exposure have usually involved solar simulators. However, they addressed the worst case scenario i.e. zenithal sun, rarely found in common outdoor activities. A non-extreme ultraviolet radiation (UV) spectrum referred as "daily UV radiation" (DUVR) with a higher UVA (320-400 nm) to UVB (280-320 nm) irradiance ratio has therefore been defined. In this study, the biological impact of an acute exposure to low physiological doses of DUVR (corresponding to 10 and 20% of the dose received per day in Paris mid-April) on a 3 dimensional reconstructed skin model, was analysed. In such conditions, epidermal and dermal morphological alterations could only be detected after the highest dose of DUVR. We then focused on oxidative stress response induced by DUVR, by analyzing the modulation of mRNA level of 24 markers in parallel in fibroblasts and keratinocytes. DUVR significantly modulated mRNA levels of these markers in both cell types. A cell type differential response was noticed: it was faster in fibroblasts, with a majority of inductions and high levels of modulation in contrast to keratinocyte response. Our results thus revealed a higher sensitivity in response to oxidative stress of dermal fibroblasts although located deeper in the skin, giving new insights into the skin biological events occurring in everyday UV exposure.