Novel Cell-Free Strategy for Therapeutic Angiogenesis: In Vitro Generated Conditioned Medium Can Replace Progenitor Cell Transplantation

• Published in: PloS one Vol. 4; no. 5; p. e5643
• Main Authors: Di Santo, Stefano, Yang, Zijiang, Wyler von Ballmoos, Moritz, Voelzmann, Jan, Diehm, Nicolas, Baumgartner, Iris, Kalka, Christoph
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 21.05.2009; Public Library of Science (PLoS)
• Subjects: Adult; Angiogenesis; Animals; Aorta; Apoptosis; Blood flow; Blood vessels; Bone marrow; Bone Marrow Cells - cytology; Bone marrow transplantation; Cardiology; Cardiovascular diseases; Cardiovascular Disorders/Peripheral Vascular Disease; Cardiovascular Disorders/Vascular Biology; CD34 antigen; CD45 antigen; Cell culture; Cell Hypoxia; Cell Line; Cell Survival; Cell-Free System; Cells (biology); Comparative analysis; Conditioning; Culture Media, Conditioned; Developmental Biology/Stem Cells; Endothelial cells; Endothelial Cells - cytology; Endothelial Cells - metabolism; Endothelium; Health aspects; Heart attacks; Heart diseases; Hematopoietic Stem Cell Mobilization; Hindlimb - blood supply; Hindlimb - pathology; Hindlimb - physiopathology; Hospitals; Human subjects; Humans; Hypoxia; Injection; Intercellular Signaling Peptides and Proteins - metabolism; Ischemia; Ischemia - chemically induced; Ischemia - pathology; Ischemia - physiopathology; Leukocytes (mononuclear); Maturation; Medicine; Muscles; Muscles - physiology; Neovascularization, Physiologic; Paracrine signalling; Perfusion; Permeability; Progenitor cells; Rats; Rats, Nude; Recovery of function; Rodents; Secretion; Stem Cell Transplantation; Stem cells; Stem Cells - cytology; Therapy; Transplantation; Vascular endothelial growth factor; Viability

Current evidence suggests that endothelial progenitor cells (EPC) contribute to ischemic tissue repair by both secretion of paracrine factors and incorporation into developing vessels. We tested the hypothesis that cell-free administration of paracrine factors secreted by cultured EPC may achieve an...