US CDC Real-Time Reverse Transcription PCR Panel for Detection of Severe Acute Respiratory Syndrome Coronavirus 2

• Published in: Emerging infectious diseases Vol. 26; no. 8; pp. 1654 - 1665
• Main Authors: Lu, Xiaoyan, Wang, Lijuan, Sakthivel, Senthilkumar K., Whitaker, Brett, Murray, Janna, Kamili, Shifaq, Lynch, Brian, Malapati, Lakshmi, Burke, Stephen A., Harcourt, Jennifer, Tamin, Azaibi, Thornburg, Natalie J., Villanueva, Julie M., Lindstrom, Stephen
• Format: Journal Article
• Language: English
• Published: United States U.S. National Center for Infectious Diseases 01.08.2020; Centers for Disease Control and Prevention
• Subjects: 2019 novel coronavirus disease; Betacoronavirus - genetics; Biomarkers - analysis; Centers for Disease Control and Prevention, U.S; coronavirus disease; Coronavirus Infections - diagnosis; Coronavirus Infections - virology; Coronavirus Nucleocapsid Proteins; Coronaviruses; COVID-19; Deoxyribonucleic acid; Disease control; DNA; DNA Primers - chemical synthesis; DNA Primers - genetics; Feces - virology; Fluoresceins - chemistry; Fluorescent Dyes - chemistry; Genetic aspects; Genomes; Health aspects; Humans; Limit of Detection; Medical laboratories; Nasopharynx - virology; Nucleocapsid Proteins - genetics; Pandemics; Phosphoproteins; Pneumonia, Viral - diagnosis; Pneumonia, Viral - virology; Polymerase chain reaction; Real-Time Polymerase Chain Reaction - methods; Real-Time Polymerase Chain Reaction - standards; Reproducibility of Results; Respiratory diseases; RNA; RNA polymerase; RNA, Viral - genetics; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Sputum - virology; Transcription (Genetics); United States; US CDC Real-Time Reverse Transcription PCR Panel for Detection of Severe Acute Respiratory Syndrome Coronavirus 2; Viruses; United States; United States > US; China; COVID-19; SARS-CoV-2; viruses; severe acute respiratory syndrome coronavirus 2; zoonoses; 2019 novel coronavirus disease; coronavirus disease; real-time RT-PCR; real-time reverse transcription PCR; respiratory infections

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was identified as the etiologic agent associated with coronavirus disease, which emerged in late 2019. In response, we developed a diagnostic panel consisting of 3 real-time reverse transcription PCR assays targeting the nucleocapsid gene...