Antibody recognition of the Pneumovirus fusion protein trimer interface

Human metapneumovirus (hMPV) is a leading cause of viral respiratory infection in children, and can cause severe lower respiratory tract infection in infants, the elderly, and immunocompromised patients. However, there remain no licensed vaccines or specific treatments for hMPV infection. Although t...

Full description

Saved in:
Bibliographic Details
Published inPLoS pathogens Vol. 16; no. 10; p. e1008942
Main Authors Huang, Jiachen, Diaz, Darren, Mousa, Jarrod J.
Format Journal Article
LanguageEnglish
Published United States Public Library of Science 09.10.2020
Public Library of Science (PLoS)
Subjects
Antibodies
Antibodies, Monoclonal - immunology
Antibodies, Neutralizing - immunology
Antibodies, Neutralizing - pharmacology
Antibodies, Viral - immunology
Antigens
BASIC BIOLOGICAL SCIENCES
Biology and Life Sciences
Crystallization
Engineering and Technology
Epidemics
Epitopes
Epitopes - immunology
F 2 region
F protein
Fusion protein
Genetic aspects
Glycan
Glycoproteins
Health aspects
Host-virus relationships
Human subjects
Humans
Identification and classification
Immune response
Immune response (humoral)
Immune system
Immunocompromised hosts
Immunology
Infants
Infections
Infectious diseases
Interferometry
Medicine and Health Sciences
Metapneumovirus - immunology
Monoclonal antibodies
Mutation
Neutralization
Neutralizing
Paramyxoviridae Infections - virology
Peptides
Physical Sciences
Pneumovirus - immunology
Proteins
Recognition
Research and Analysis Methods
Respiratory diseases
Respiratory syncytial virus
Respiratory Syncytial Virus, Human - immunology
Respiratory tract
Respiratory tract diseases
RNA viruses
Severe acute respiratory syndrome coronavirus 2
Trimers
Vaccines
Veterinary colleges
Veterinary medicine
Viral Fusion Proteins - immunology
Viral infections
Viral proteins
Viruses
United States
United States > US
Online AccessGet full text

Cover

Abstract Human metapneumovirus (hMPV) is a leading cause of viral respiratory infection in children, and can cause severe lower respiratory tract infection in infants, the elderly, and immunocompromised patients. However, there remain no licensed vaccines or specific treatments for hMPV infection. Although the hMPV fusion (F) protein is the sole target of neutralizing antibodies, the immunological properties of hMPV F remain poorly understood. To further define the humoral immune response to the hMPV F protein, we isolated two new human monoclonal antibodies (mAbs), MPV458 and MPV465. Both mAbs are neutralizing in vitro and were determined to target a unique antigenic site using competitive biolayer interferometry. We determined both MPV458 and MPV465 have higher affinity for monomeric hMPV F than trimeric hMPV F. MPV458 was co-crystallized with hMPV F, and the mAb primarily interacts with an alpha helix on the F2 region of the hMPV F protein. Surprisingly, the major epitope for MPV458 lies within the trimeric interface of the hMPV F protein, suggesting significant breathing of the hMPV F protein must occur for host immune recognition of the novel epitope. In addition, significant glycan interactions were observed with a somatically mutated light chain framework residue. The data presented identifies a novel epitope on the hMPV F protein for epitope-based vaccine design, and illustrates a new mechanism for human antibody neutralization of viral glycoproteins.
AbstractList Human metapneumovirus (hMPV) is a leading cause of viral respiratory infection in children, and can cause severe lower respiratory tract infection in infants, the elderly, and immunocompromised patients. However, there remain no licensed vaccines or specific treatments for hMPV infection. Although the hMPV fusion (F) protein is the sole target of neutralizing antibodies, the immunological properties of hMPV F remain poorly understood. To further define the humoral immune response to the hMPV F protein, we isolated two new human monoclonal antibodies (mAbs), MPV458 and MPV465. Both mAbs are neutralizing in vitro and were determined to target a unique antigenic site using competitive biolayer interferometry. We determined both MPV458 and MPV465 have higher affinity for monomeric hMPV F than trimeric hMPV F. MPV458 was co-crystallized with hMPV F, and the mAb primarily interacts with an alpha helix on the F2 region of the hMPV F protein. Surprisingly, the major epitope for MPV458 lies within the trimeric interface of the hMPV F protein, suggesting significant breathing of the hMPV F protein must occur for host immune recognition of the novel epitope. In addition, significant glycan interactions were observed with a somatically mutated light chain framework residue. The data presented identifies a novel epitope on the hMPV F protein for epitope-based vaccine design, and illustrates a new mechanism for human antibody neutralization of viral glycoproteins.
Human metapneumovirus (hMPV) is a leading cause of viral respiratory infection in children, and can cause severe lower respiratory tract infection in infants, the elderly, and immunocompromised patients. However, there remain no licensed vaccines or specific treatments for hMPV infection. Although the hMPV fusion (F) protein is the sole target of neutralizing antibodies, the immunological properties of hMPV F remain poorly understood. To further define the humoral immune response to the hMPV F protein, we isolated two new human monoclonal antibodies (mAbs), MPV458 and MPV465. Both mAbs are neutralizing in vitro and were determined to target a unique antigenic site using competitive biolayer interferometry. We determined both MPV458 and MPV465 have higher affinity for monomeric hMPV F than trimeric hMPV F. MPV458 was co-crystallized with hMPV F, and the mAb primarily interacts with an alpha helix on the F2 region of the hMPV F protein. Surprisingly, the major epitope for MPV458 lies within the trimeric interface of the hMPV F protein, suggesting significant breathing of the hMPV F protein must occur for host immune recognition of the novel epitope. In addition, significant glycan interactions were observed with a somatically mutated light chain framework residue. The data presented identifies a novel epitope on the hMPV F protein for epitope-based vaccine design, and illustrates a new mechanism for human antibody neutralization of viral glycoproteins.Human metapneumovirus (hMPV) is a leading cause of viral respiratory infection in children, and can cause severe lower respiratory tract infection in infants, the elderly, and immunocompromised patients. However, there remain no licensed vaccines or specific treatments for hMPV infection. Although the hMPV fusion (F) protein is the sole target of neutralizing antibodies, the immunological properties of hMPV F remain poorly understood. To further define the humoral immune response to the hMPV F protein, we isolated two new human monoclonal antibodies (mAbs), MPV458 and MPV465. Both mAbs are neutralizing in vitro and were determined to target a unique antigenic site using competitive biolayer interferometry. We determined both MPV458 and MPV465 have higher affinity for monomeric hMPV F than trimeric hMPV F. MPV458 was co-crystallized with hMPV F, and the mAb primarily interacts with an alpha helix on the F2 region of the hMPV F protein. Surprisingly, the major epitope for MPV458 lies within the trimeric interface of the hMPV F protein, suggesting significant breathing of the hMPV F protein must occur for host immune recognition of the novel epitope. In addition, significant glycan interactions were observed with a somatically mutated light chain framework residue. The data presented identifies a novel epitope on the hMPV F protein for epitope-based vaccine design, and illustrates a new mechanism for human antibody neutralization of viral glycoproteins.
Human metapneumovirus (hMPV) is a leading cause of viral respiratory infection in children, and can cause severe lower respiratory tract infection in infants, the elderly, and immunocompromised patients. However, there remain no licensed vaccines or specific treatments for hMPV infection. Although the hMPV fusion (F) protein is the sole target of neutralizing antibodies, the immunological properties of hMPV F remain poorly understood. To further define the humoral immune response to the hMPV F protein, we isolated two new human monoclonal antibodies (mAbs), MPV458 and MPV465. Both mAbs are neutralizing in vitro and were determined to target a unique antigenic site using competitive biolayer interferometry. We determined both MPV458 and MPV465 have higher affinity for monomeric hMPV F than trimeric hMPV F. MPV458 was co-crystallized with hMPV F, and the mAb primarily interacts with an alpha helix on the F2 region of the hMPV F protein. Surprisingly, the major epitope for MPV458 lies within the trimeric interface of the hMPV F protein, suggesting significant breathing of the hMPV F protein must occur for host immune recognition of the novel epitope. In addition, significant glycan interactions were observed with a somatically mutated light chain framework residue. The data presented identifies a novel epitope on the hMPV F protein for epitope-based vaccine design, and illustrates a new mechanism for human antibody neutralization of viral glycoproteins. Human metapneumovirus (hMPV) is a common cause of lower respiratory tract infection in children, the elderly, and the immunocompromised. There is currently no approved vaccine or therapeutic to prevent or treat hMPV infection. The hMPV fusion (F) protein is the sole target of antibodies that neutralize hMPV. Here we describe new human mAbs that bind a unique epitope on the hMPV F protein. The mAbs were mapped to a new antigenic site on the hMPV F protein located within the trimeric interface of the hMPV F protein based on competitive biolayer interferometry and X-ray crystallographic experiments. This is the first example of a human mAb that binds inside the trimeric interface of a viral glycoprotein and neutralizes the virus. The results suggest such antibodies may exist for other viral fusion proteins, and identify a new epitope on the hMPV F protein for epitope-based vaccine design.
Audience Academic
Author Huang, Jiachen
Mousa, Jarrod J.
Diaz, Darren
AuthorAffiliation 2 Center for Vaccines and Immunology, College of Veterinary Medicine, University of Georgia, Athens, GA, United States of America
1 Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA, United States of America
University of Texas Medical Branch / Galveston National Laboratory, UNITED STATES
AuthorAffiliation_xml – name: University of Texas Medical Branch / Galveston National Laboratory, UNITED STATES
– name: 2 Center for Vaccines and Immunology, College of Veterinary Medicine, University of Georgia, Athens, GA, United States of America
– name: 1 Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA, United States of America
Author_xml – sequence: 1
  givenname: Jiachen
  surname: Huang
  fullname: Huang, Jiachen
– sequence: 2
  givenname: Darren
  orcidid: 0000-0002-0114-3973
  surname: Diaz
  fullname: Diaz, Darren
– sequence: 3
  givenname: Jarrod J.
  orcidid: 0000-0003-4709-2478
  surname: Mousa
  fullname: Mousa, Jarrod J.
BackLink https://www.ncbi.nlm.nih.gov/pubmed/33035266$$D View this record in MEDLINE/PubMed
https://www.osti.gov/servlets/purl/1834810$$D View this record in Osti.gov
BookMark eNqVk19v0zAUxSM0xLbCN0AQwQs8tNix49g8IFUTjEoTIP48W4573bpK7WI7E_v2ODRF6zQhoTwksn_n-N4T3_PixHkHRfEUoxkmDX6z8X1wqpvtdirNMEJc0OpBcYbrmkwb0tCTW9-nxXmMG4QoJpg9Kk4JQaSuGDsrLucu2dYvb8oA2q-cTda70psyraH84qDf-msb-liaPg47u-ATWFemYLcQSusSBKM0PC4eGtVFeDK-J8WPD--_X3ycXn2-XFzMr6a6qWmaUsOIxnVFAWvFGaOagECEVoxj1WJmeEsrwWuDGeLQNohgUAybRjABrWjIpHi-9911PsoxgygrypDghBCaicWeWHq1kbtcpwo30isr_yz4sJIqJKs7kKhlVaU5MrRFtNZtiytiloRXlSC5KZW93o2n9e0WlhpcCqo7Mj3ecXYtV_5aNrXgtGHZ4MXewMdkZdQ2gV5r7xzoJDEnlGOUoVfjKcH_7CEmubVRQ9cpB74fmqNC1IKQKqMv76D3RzBSK5W7tM74XJweTOWc0Tr_fZw7nBSze6j8LGFrc41gbF4_Erw-EmQmwa-0Un2McvHt63-wn47ZZ7dT_hvv4ZZm4O0e0MHHGMDIHKQabmqu2HYSIzmMxCELOYyEHEcii-kd8cH_n7LfO54OoQ
CitedBy_id crossref_primary_10_1146_annurev_immunol_042718_041309
crossref_primary_10_1016_j_immuni_2022_07_003
crossref_primary_10_1016_j_str_2024_08_022
crossref_primary_10_4049_jimmunol_2101171
crossref_primary_10_1038_s41467_022_28931_3
crossref_primary_10_1172_JCI159062
crossref_primary_10_3389_fimmu_2022_941865
crossref_primary_10_1038_s41598_023_42692_z
crossref_primary_10_1038_s41467_022_30205_x
crossref_primary_10_1016_S2666_5247_23_00134_9
crossref_primary_10_1016_j_celrep_2022_111399
crossref_primary_10_1038_s41467_024_50659_5
crossref_primary_10_1128_jvi_00896_22
crossref_primary_10_1038_s41467_024_54287_x
crossref_primary_10_1128_JVI_00593_21
crossref_primary_10_1128_mbio_02122_23
crossref_primary_10_1128_jvi_01052_23
crossref_primary_10_1073_pnas_2203326119
crossref_primary_10_1016_j_chom_2023_07_002
crossref_primary_10_1080_21505594_2023_2284515
crossref_primary_10_1128_IAI_00747_20
crossref_primary_10_4049_jimmunol_2200349
crossref_primary_10_1016_j_coviro_2023_101337
crossref_primary_10_1038_s41467_024_45404_x
crossref_primary_10_17116_labs20231203120
Cites_doi 10.3201/eid0909.030304
10.1111/tid.12074
10.1016/j.virusres.2011.06.014
10.1016/j.cell.2019.04.011
10.1128/JVI.00318-06
10.1107/S010876731009728X
10.1086/367901
10.1371/journal.ppat.1005303
10.1016/j.cell.2019.03.048
10.1086/379200
10.3201/eid1003.030513
10.1126/science.1243283
10.1371/journal.ppat.1005859
10.1128/JVI.00342-19
10.1073/pnas.0801433106
10.1099/vir.0.2008/005199-0
10.1093/nar/gkn316
10.1073/pnas.1609449113
10.3390/v5010087
10.1038/89098
10.1038/s41467-019-09807-5
10.1016/j.jamda.2019.03.015
10.1016/j.jcv.2014.01.003
10.1016/j.ijid.2014.03.1394
10.1016/j.vaccine.2010.07.011
10.1126/science.1254426
10.1016/j.canlet.2016.05.035
10.1038/nsmb.2250
10.1371/journal.ppat.1006837
10.1038/nm.4224
10.1038/s41467-017-01708-9
10.1086/379771
10.1126/scitranslmed.aac4241
10.1016/j.healun.2005.02.014
10.1128/JCM.00152-06
10.1099/vir.0.82753-0
10.1128/JVI.00106-07
10.1016/j.jcv.2007.08.015
10.1016/j.virol.2005.10.016
10.1128/JVI.06706-11
10.1128/JVI.01579-10
10.1086/649587
10.1128/microbiolspec.AID-0027-2014
10.1038/nmicrobiol.2016.272
10.1016/j.arcped.2019.10.002
10.1038/nmicrobiol.2016.271
10.1038/nature12442
10.1128/JVI.02848-13
10.1038/sj.bmt.1703849
10.1126/science.1234914
10.1128/JVI.01208-08
10.1016/j.jim.2007.09.017
10.1128/JVI.01133-12
10.1126/science.aav9033
10.1128/CVI.00398-09
10.1016/j.coviro.2013.03.006
10.1038/ncomms9143
10.1016/j.chom.2019.04.003
10.3389/fimmu.2019.02778
10.1056/NEJMoa025472
10.1093/infdis/jix167
10.1128/JVI.78.23.12877-12887.2004
10.3201/eid1004.030393
10.1128/JVI.01287-06
10.1038/ncomms9167
ContentType Journal Article
Copyright COPYRIGHT 2020 Public Library of Science
2020 Huang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
2020 Huang et al 2020 Huang et al
Copyright_xml – notice: COPYRIGHT 2020 Public Library of Science
– notice: 2020 Huang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
– notice: 2020 Huang et al 2020 Huang et al
CorporateAuthor Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
CorporateAuthor_xml – name: Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
DBID AAYXX
CITATION
CGR
CUY
CVF
ECM
EIF
NPM
ISN
ISR
3V.
7QL
7U9
7X7
7XB
88E
8FE
8FH
8FI
8FJ
8FK
ABUWG
AEUYN
AFKRA
AZQEC
BBNVY
BENPR
BHPHI
C1K
CCPQU
COVID
DWQXO
FYUFA
GHDGH
GNUQQ
H94
HCIFZ
K9.
LK8
M0S
M1P
M7P
PHGZM
PHGZT
PIMPY
PJZUB
PKEHL
PPXIY
PQEST
PQGLB
PQQKQ
PQUKI
PRINS
7X8
OIOZB
OTOTI
5PM
DOA
DOI 10.1371/journal.ppat.1008942
DatabaseName CrossRef
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
Gale In Context: Canada
Gale In Context: Science
ProQuest Central (Corporate)
Bacteriology Abstracts (Microbiology B)
Virology and AIDS Abstracts
Health & Medical Collection
ProQuest Central (purchase pre-March 2016)
Medical Database (Alumni Edition)
ProQuest SciTech Collection
ProQuest Natural Science Collection
Hospital Premium Collection
Hospital Premium Collection (Alumni Edition)
ProQuest Central (Alumni) (purchase pre-March 2016)
ProQuest Central (Alumni)
ProQuest One Sustainability (subscription)
ProQuest Central UK/Ireland
ProQuest Central Essentials
Biological Science Collection (ProQuest)
ProQuest Central
Natural Science Collection (ProQuest)
Environmental Sciences and Pollution Management
ProQuest One Community College
Coronavirus Research Database
ProQuest Central Korea
Health Research Premium Collection
Health Research Premium Collection (Alumni)
ProQuest Central Student
AIDS and Cancer Research Abstracts
SciTech Premium Collection (ProQuest)
ProQuest Health & Medical Complete (Alumni)
ProQuest Biological Science Collection
ProQuest Health & Medical Collection
Medical Database
Biological Science Database
ProQuest Central Premium
ProQuest One Academic
Publicly Available Content Database
ProQuest Health & Medical Research Collection
ProQuest One Academic Middle East (New)
ProQuest One Health & Nursing
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Applied & Life Sciences
ProQuest One Academic
ProQuest One Academic UKI Edition
ProQuest Central China
MEDLINE - Academic
OSTI.GOV - Hybrid
OSTI.GOV
PubMed Central (Full Participant titles)
DOAJ Directory of Open Access Journals
DatabaseTitle CrossRef
MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
Publicly Available Content Database
ProQuest Central Student
ProQuest One Academic Middle East (New)
ProQuest Central Essentials
ProQuest Health & Medical Complete (Alumni)
ProQuest Central (Alumni Edition)
SciTech Premium Collection
ProQuest One Community College
ProQuest One Health & Nursing
ProQuest Natural Science Collection
ProQuest Central China
Environmental Sciences and Pollution Management
ProQuest Central
ProQuest One Applied & Life Sciences
ProQuest One Sustainability
ProQuest Health & Medical Research Collection
Health Research Premium Collection
Health and Medicine Complete (Alumni Edition)
Natural Science Collection
ProQuest Central Korea
Bacteriology Abstracts (Microbiology B)
Health & Medical Research Collection
Biological Science Collection
AIDS and Cancer Research Abstracts
ProQuest Central (New)
ProQuest Medical Library (Alumni)
Virology and AIDS Abstracts
ProQuest Biological Science Collection
ProQuest One Academic Eastern Edition
Coronavirus Research Database
ProQuest Hospital Collection
Health Research Premium Collection (Alumni)
Biological Science Database
ProQuest SciTech Collection
ProQuest Hospital Collection (Alumni)
ProQuest Health & Medical Complete
ProQuest Medical Library
ProQuest One Academic UKI Edition
ProQuest One Academic
ProQuest One Academic (New)
ProQuest Central (Alumni)
MEDLINE - Academic
DatabaseTitleList Publicly Available Content Database


MEDLINE - Academic



MEDLINE
Database_xml – sequence: 1
  dbid: DOA
  name: DOAJ Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
– sequence: 2
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 3
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
– sequence: 4
  dbid: BENPR
  name: ProQuest Central
  url: https://www.proquest.com/central
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Biology
Veterinary Medicine
DocumentTitleAlternate Antibody recognition of the Pneumovirus fusion protein trimer interface
EISSN 1553-7374
ExternalDocumentID 2460983334
oai_doaj_org_article_0b622c80f4b045cbb123fd382293faca
PMC7598476
1834810
A645330129
33035266
10_1371_journal_ppat_1008942
Genre Research Support, U.S. Gov't, Non-P.H.S
Research Support, Non-U.S. Gov't
Journal Article
Research Support, N.I.H., Extramural
GeographicLocations United States
United States--US
GeographicLocations_xml – name: United States
– name: United States--US
GrantInformation_xml – fundername: NIH HHS
  grantid: K01 OD026569
– fundername: NIAID NIH HHS
  grantid: R01 AI143865
– fundername: NCRR NIH HHS
  grantid: S10 RR028976
– fundername: NCATS NIH HHS
  grantid: UL1 TR002378
– fundername: NCRR NIH HHS
  grantid: S10 RR025528
– fundername: ;
  grantid: 1K01OD026569
– fundername: ;
  grantid: 1R01AI143865
GroupedDBID ---
123
29O
2WC
53G
5VS
7X7
88E
8FE
8FH
8FI
8FJ
AAFWJ
AAUCC
AAWOE
AAYXX
ABDBF
ABUWG
ACGFO
ACIHN
ACPRK
ACUHS
ADBBV
AEAQA
AENEX
AEUYN
AFKRA
AFPKN
AFRAH
AHMBA
ALMA_UNASSIGNED_HOLDINGS
AOIJS
B0M
BAWUL
BBNVY
BCNDV
BENPR
BHPHI
BPHCQ
BVXVI
BWKFM
CCPQU
CITATION
CS3
DIK
DU5
E3Z
EAP
EAS
EBD
EMK
EMOBN
ESX
F5P
FPL
FYUFA
GROUPED_DOAJ
GX1
HCIFZ
HMCUK
HYE
IAO
IHR
INH
INR
ISN
ISR
ITC
KQ8
LK8
M1P
M48
M7P
MM.
O5R
O5S
OK1
OVT
P2P
PGMZT
PHGZM
PHGZT
PIMPY
PQQKQ
PROAC
PSQYO
PV9
QF4
QN7
RNS
RPM
RZL
SV3
TR2
TUS
UKHRP
WOW
~8M
ADRAZ
CGR
CUY
CVF
ECM
EIF
H13
IPNFZ
NPM
PJZUB
PPXIY
PQGLB
RIG
WOQ
PMFND
3V.
7QL
7U9
7XB
8FK
AZQEC
C1K
COVID
DWQXO
GNUQQ
H94
K9.
PKEHL
PQEST
PQUKI
PRINS
7X8
AAPBV
ABPTK
M~E
OIOZB
OTOTI
UMP
5PM
PUEGO
-
ADACO
BBAFP
ID FETCH-LOGICAL-c754t-4f63c1524e1ca8664c3e90342681ab16f8b42985f1608eb7031ea61f7969eb973
IEDL.DBID M48
ISSN 1553-7374
1553-7366
IngestDate Fri Nov 26 17:53:16 EST 2021
Wed Aug 27 01:31:51 EDT 2025
Thu Aug 21 14:02:31 EDT 2025
Fri May 19 00:37:20 EDT 2023
Tue Aug 05 10:27:17 EDT 2025
Fri Jul 25 12:08:05 EDT 2025
Tue Jun 17 21:37:29 EDT 2025
Tue Jun 10 20:32:39 EDT 2025
Fri Jun 27 05:12:51 EDT 2025
Fri Jun 27 05:14:34 EDT 2025
Mon Jul 21 06:01:41 EDT 2025
Tue Jul 01 04:24:22 EDT 2025
Thu Apr 24 23:09:11 EDT 2025
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 10
Language English
License This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Creative Commons Attribution License
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c754t-4f63c1524e1ca8664c3e90342681ab16f8b42985f1608eb7031ea61f7969eb973
Notes new_version
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
USDOE Office of Science (SC), Basic Energy Sciences (BES)
JH and JJM are inventors on a patent application describing the monoclonal antibodies and the epitope.
ORCID 0000-0002-0114-3973
0000-0003-4709-2478
0000000201143973
0000000347092478
OpenAccessLink http://journals.scholarsportal.info/openUrl.xqy?doi=10.1371/journal.ppat.1008942
PMID 33035266
PQID 2460983334
PQPubID 1436335
ParticipantIDs plos_journals_2460983334
doaj_primary_oai_doaj_org_article_0b622c80f4b045cbb123fd382293faca
pubmedcentral_primary_oai_pubmedcentral_nih_gov_7598476
osti_scitechconnect_1834810
proquest_miscellaneous_2449959332
proquest_journals_2460983334
gale_infotracmisc_A645330129
gale_infotracacademiconefile_A645330129
gale_incontextgauss_ISR_A645330129
gale_incontextgauss_ISN_A645330129
pubmed_primary_33035266
crossref_citationtrail_10_1371_journal_ppat_1008942
crossref_primary_10_1371_journal_ppat_1008942
ProviderPackageCode CITATION
AAYXX
PublicationCentury 2000
PublicationDate 2020-10-09
PublicationDateYYYYMMDD 2020-10-09
PublicationDate_xml – month: 10
  year: 2020
  text: 2020-10-09
  day: 09
PublicationDecade 2020
PublicationPlace United States
PublicationPlace_xml – name: United States
– name: San Francisco
– name: San Francisco, CA USA
PublicationTitle PLoS pathogens
PublicationTitleAlternate PLoS Pathog
PublicationYear 2020
Publisher Public Library of Science
Public Library of Science (PLoS)
Publisher_xml – name: Public Library of Science
– name: Public Library of Science (PLoS)
References A Watanabe (ppat.1008942.ref062) 2019; 177
S Biacchesi (ppat.1008942.ref036) 2004; 78
J Lee (ppat.1008942.ref063) 2016; 22
B Lormeau (ppat.1008942.ref017) 2019; 26
RG Cox (ppat.1008942.ref034) 2015; 11
V Más (ppat.1008942.ref043) 2016; 12
A Krarup (ppat.1008942.ref040) 2015; 6
DP Shah (ppat.1008942.ref011) 2016; 379
D Corti (ppat.1008942.ref051) 2013; 501
N Principi (ppat.1008942.ref001) 2014; 59
PKS Chan (ppat.1008942.ref020) 2004; 10
D Seynaeve (ppat.1008942.ref015) 2020; 21
PD Adams (ppat.1008942.ref074) 2010; 66
JJ Mousa (ppat.1008942.ref045) 2017; 2
G Cseke (ppat.1008942.ref032) 2009; 106
C Larcher (ppat.1008942.ref008) 2005; 24
A Chang (ppat.1008942.ref031) 2012; 86
G Bajic (ppat.1008942.ref061) 2019; 25
PKS Chan (ppat.1008942.ref022) 2003; 9
J Dong (ppat.1008942.ref060) 2020
JS McLellan (ppat.1008942.ref066) 2013; 340
JS McLellan (ppat.1008942.ref070) 2010; 84
JE Schuster (ppat.1008942.ref050) 2014; 211
J Lüsebrink (ppat.1008942.ref057) 2010; 17
MB Battles (ppat.1008942.ref038) 2017; 8
T Tiller (ppat.1008942.ref071) 2008; 329
J Huang (ppat.1008942.ref035) 2019; 10
X Wen (ppat.1008942.ref052) 2017; 2
X Wen (ppat.1008942.ref055) 2012; 19
MH Skiadopoulos (ppat.1008942.ref030) 2006; 345
PA Cane (ppat.1008942.ref009) 2003; 31
S Bangaru (ppat.1008942.ref064) 2019; 177
JJ Mousa (ppat.1008942.ref058) 2016; 113
JJ Mousa (ppat.1008942.ref053) 2018; 14
JH Lee (ppat.1008942.ref069) 2015; 6
MB Klein (ppat.1008942.ref018) 2010; 201
SA Smith (ppat.1008942.ref056) 2015; 3
C Masante (ppat.1008942.ref027) 2014; 88
SJ Wu (ppat.1008942.ref059) 2007; 88
K Kan-o (ppat.1008942.ref019) 2018; 215
B Huck (ppat.1008942.ref012) 2006; 44
C Dokos (ppat.1008942.ref013) 2013; 15
SA Peña (ppat.1008942.ref014) 2019; 25
X Brochet (ppat.1008942.ref072) 2008; 36
C Dokos (ppat.1008942.ref010) 2013; 15
SA Hashemi (ppat.1008942.ref023) 2020
Y Murata (ppat.1008942.ref037) 2010; 28
LEM Haas (ppat.1008942.ref007) 2013; 5
N Lee (ppat.1008942.ref021) 2007; 40
P Emsley (ppat.1008942.ref075) 2004; 60
BG van den Hoogen (ppat.1008942.ref005) 2003; 188
S Panda (ppat.1008942.ref003) 2014; 25
S Thammawat (ppat.1008942.ref028) 2008; 82
W Kabsch (ppat.1008942.ref073) 2010; 66
(CDC) C for DC and P (ppat.1008942.ref016) 2013; 62
J V Williams (ppat.1008942.ref025) 2004; 350
ND Ulbrandt (ppat.1008942.ref047) 2006; 80
F Touzard-Romo (ppat.1008942.ref024) 2020; 103
RG Cox (ppat.1008942.ref033) 2012; 86
BG Van Den Hoogen (ppat.1008942.ref026) 2004; 10
MSA Gilman (ppat.1008942.ref065) 2019; 10
BG van den Hoogen (ppat.1008942.ref002) 2001; 7
R Piyaratna (ppat.1008942.ref029) 2011; 160
MSA Gilman (ppat.1008942.ref044) 2016; 1
ND Ulbrandt (ppat.1008942.ref048) 2008; 89
Y Bar-Peled (ppat.1008942.ref054) 2019; 93
JB Munro (ppat.1008942.ref067) 2014; 346
RM Schowalter (ppat.1008942.ref042) 2006; 80
MC Crank (ppat.1008942.ref041) 2019; 365
JS McLellan (ppat.1008942.ref039) 2013; 342
AR Falsey (ppat.1008942.ref004) 2003; 187
SA Madhi (ppat.1008942.ref006) 2003; 37
JO Ngwuta (ppat.1008942.ref046) 2015; 7
E Baquero (ppat.1008942.ref068) 2013; 3
J V Williams (ppat.1008942.ref049) 2007; 81
References_xml – volume: 9
  start-page: 1058
  year: 2003
  ident: ppat.1008942.ref022
  article-title: Human metapneumovirus detection in patients with severe acute respiratory syndrome
  publication-title: Emerg Infect Dis
  doi: 10.3201/eid0909.030304
– volume: 15
  start-page: E97
  year: 2013
  ident: ppat.1008942.ref013
  article-title: Fatal human metapneumovirus infection following allogeneic hematopoietic stem cell transplantation
  publication-title: Transpl Infect Dis
  doi: 10.1111/tid.12074
– volume: 160
  start-page: 200
  year: 2011
  ident: ppat.1008942.ref029
  article-title: Genomic analysis of four human metapneumovirus prototypes
  publication-title: Virus Res
  doi: 10.1016/j.virusres.2011.06.014
– volume: 177
  start-page: 1136
  year: 2019
  ident: ppat.1008942.ref064
  article-title: A Site of Vulnerability on the Influenza Virus Hemagglutinin Head Domain Trimer Interface
  publication-title: Cell
  doi: 10.1016/j.cell.2019.04.011
– volume: 80
  start-page: 7799
  year: 2006
  ident: ppat.1008942.ref047
  article-title: Isolation and characterization of monoclonal antibodies which neutralize human metapneumovirus in vitro and in vivo
  publication-title: J Virol
  doi: 10.1128/JVI.00318-06
– volume: 66
  start-page: 125
  year: 2010
  ident: ppat.1008942.ref073
  publication-title: Xds. Acta Cryst
  doi: 10.1107/S010876731009728X
– volume: 187
  start-page: 785
  year: 2003
  ident: ppat.1008942.ref004
  article-title: Human metapneumovirus infections in young and elderly adults
  publication-title: J Infect Dis
  doi: 10.1086/367901
– volume: 11
  start-page: e1005303
  year: 2015
  ident: ppat.1008942.ref034
  article-title: Human metapneumovirus Is capable of entering cells by fusion with endosomal membranes
  publication-title: PLoS Pathog
  doi: 10.1371/journal.ppat.1005303
– volume: 177
  start-page: 1124
  year: 2019
  ident: ppat.1008942.ref062
  article-title: Antibodies to a Conserved Influenza Head Interface Epitope Protect by an IgG Subtype-Dependent Mechanism
  publication-title: Cell
  doi: 10.1016/j.cell.2019.03.048
– volume: 188
  start-page: 1571
  year: 2003
  ident: ppat.1008942.ref005
  article-title: Prevalence and clinical symptoms of human metapneumovirus infection in hospitalized patients
  publication-title: J Infect Dis
  doi: 10.1086/379200
– volume: 10
  start-page: 497
  year: 2004
  ident: ppat.1008942.ref020
  article-title: Human metapneumovirus-associated atypical pneumonia and SARS
  publication-title: Emerg Infect Dis
  doi: 10.3201/eid1003.030513
– volume: 342
  start-page: 592
  year: 2013
  ident: ppat.1008942.ref039
  article-title: Structure-based design of a fusion glycoprotein vaccine for respiratory syncytial virus
  publication-title: Science
  doi: 10.1126/science.1243283
– volume: 12
  start-page: e1005859
  year: 2016
  ident: ppat.1008942.ref043
  article-title: Engineering, structure and immunogenicity of the human metapneumovirus F protein in the postfusion conformation
  publication-title: PLoS Pathog
  doi: 10.1371/journal.ppat.1005859
– volume: 93
  start-page: e00342
  year: 2019
  ident: ppat.1008942.ref054
  article-title: A potent neutralizing site III-specific human antibody neutralizes human metapneumovirus in vivo
  publication-title: J Virol
  doi: 10.1128/JVI.00342-19
– volume: 106
  start-page: 1566
  year: 2009
  ident: ppat.1008942.ref032
  article-title: Integrin alphavbeta1 promotes infection by human metapneumovirus
  publication-title: PNAS
  doi: 10.1073/pnas.0801433106
– volume: 89
  start-page: 3113
  year: 2008
  ident: ppat.1008942.ref048
  article-title: Identification of antibody neutralization epitopes on the fusion protein of human metapneumovirus
  publication-title: J Gen Virol
  doi: 10.1099/vir.0.2008/005199-0
– volume: 36
  start-page: 503
  year: 2008
  ident: ppat.1008942.ref072
  article-title: IMGT/V-QUEST: the highly customized and integrated system for IG and TR standardized V-J and V-D-J sequence analysis
  publication-title: Nucleic Acids Res
  doi: 10.1093/nar/gkn316
– volume: 113
  start-page: E6849
  year: 2016
  ident: ppat.1008942.ref058
  article-title: Structural basis for nonneutralizing antibody competition at antigenic site II of the respiratory syncytial virus fusion protein
  publication-title: PNAS
  doi: 10.1073/pnas.1609449113
– volume: 5
  start-page: 87
  year: 2013
  ident: ppat.1008942.ref007
  article-title: Human metapneumovirus in adults
  publication-title: Viruses
  doi: 10.3390/v5010087
– year: 2020
  ident: ppat.1008942.ref060
  article-title: Anti-influenza H7 human antibody targets antigenic site in hemagglutinin head domain interface
  publication-title: J Clin Invest
– volume: 66
  start-page: 213
  year: 2010
  ident: ppat.1008942.ref074
  article-title: PHENIX: a comprehensive Python-based system for macromolecular structure solution
  publication-title: Acta Cryst
– volume: 7
  start-page: 719
  year: 2001
  ident: ppat.1008942.ref002
  article-title: A newly discovered human pneumovirus isolated from young children with respiratory tract disease
  publication-title: Nat Med
  doi: 10.1038/89098
– volume: 10
  start-page: 2105
  year: 2019
  ident: ppat.1008942.ref065
  article-title: Transient opening of trimeric prefusion RSV F proteins
  publication-title: Nat Commun
  doi: 10.1038/s41467-019-09807-5
– volume: 21
  start-page: 104
  year: 2020
  ident: ppat.1008942.ref015
  article-title: Outbreak of Human Metapneumovirus in a Nursing Home: A Clinical Perspective
  publication-title: J Am Med Dir Assoc
  doi: 10.1016/j.jamda.2019.03.015
– volume: 59
  start-page: 141
  year: 2014
  ident: ppat.1008942.ref001
  article-title: Paediatric human metapneumovirus infection: Epidemiology, prevention and therapy
  publication-title: J Clin Virol
  doi: 10.1016/j.jcv.2014.01.003
– volume: 25
  start-page: 45
  year: 2014
  ident: ppat.1008942.ref003
  article-title: Human metapneumovirus: Review of an important respiratory pathogen
  publication-title: Int J Infect Dis
  doi: 10.1016/j.ijid.2014.03.1394
– year: 2020
  ident: ppat.1008942.ref023
  article-title: Report of death in children with SARS-CoV-2 and Human metapneumovirus (hMPV) co-infection: is hMPV the trigger?
  publication-title: J Med Virol
– volume: 28
  start-page: 6242
  year: 2010
  ident: ppat.1008942.ref037
  article-title: Humoral response to the central unglycosylated region of the respiratory syncytial virus attachment protein
  publication-title: Vaccine
  doi: 10.1016/j.vaccine.2010.07.011
– volume: 346
  start-page: 759
  year: 2014
  ident: ppat.1008942.ref067
  article-title: Conformational dynamics of single HIV-1 envelope trimers on the surface of native virions
  publication-title: Science
  doi: 10.1126/science.1254426
– volume: 379
  start-page: 100
  year: 2016
  ident: ppat.1008942.ref011
  article-title: Human metapneumovirus infections in hematopoietic cell transplant recipients and hematologic malignancy patients: A systematic review
  publication-title: Cancer Lett
  doi: 10.1016/j.canlet.2016.05.035
– volume: 19
  start-page: 461
  year: 2012
  ident: ppat.1008942.ref055
  article-title: Structure of the human metapneumovirus fusion protein with neutralizing antibody identifies a pneumovirus antigenic site
  publication-title: Nat Struc Mol Biol
  doi: 10.1038/nsmb.2250
– volume: 14
  start-page: e1006837
  year: 2018
  ident: ppat.1008942.ref053
  article-title: Human antibody recognition of antigenic site IV on Pneumovirus fusion proteins
  publication-title: PLoS Pathog
  doi: 10.1371/journal.ppat.1006837
– volume: 22
  start-page: 1456
  year: 2016
  ident: ppat.1008942.ref063
  article-title: Molecular-level analysis of the serum antibody repertoire in young adults before and after seasonal influenza vaccination
  publication-title: Nat Med
  doi: 10.1038/nm.4224
– volume: 25
  year: 2019
  ident: ppat.1008942.ref014
  article-title: Severe respiratory illness associated with human metapneumovirus in nursing home, New Mexico, USA
  publication-title: Emerg Infect Dis
– volume: 8
  start-page: 1528
  year: 2017
  ident: ppat.1008942.ref038
  article-title: Structure and immunogenicity of pre-fusion-stabilized human metapneumovirus F glycoprotein
  publication-title: Nat Commun
  doi: 10.1038/s41467-017-01708-9
– volume: 37
  start-page: 1705
  year: 2003
  ident: ppat.1008942.ref006
  article-title: Human metapneumovirus-associated lower respiratory tract infections among hospitalized human immunodeficiency virus type 1 (HIV-1)-infected and HIV-1-uninfected African infants
  publication-title: Clin Infect Dis
  doi: 10.1086/379771
– volume: 7
  start-page: 309ra162
  year: 2015
  ident: ppat.1008942.ref046
  article-title: Prefusion F–specific antibodies determine the magnitude of RSV neutralizing activity in human sera
  publication-title: Sci Transl Med
  doi: 10.1126/scitranslmed.aac4241
– volume: 24
  start-page: 1891
  year: 2005
  ident: ppat.1008942.ref008
  article-title: Human metapneumovirus infection in lung transplant recipients: Clinical presentation and epidemiology
  publication-title: J Hear Lung Transpl
  doi: 10.1016/j.healun.2005.02.014
– volume: 44
  start-page: 2300
  year: 2006
  ident: ppat.1008942.ref012
  article-title: Human metapneumovirus infection in a hematopoietic stem cell transplant recipient with relapsed multiple myeloma and rapidly progressing lung cancer
  publication-title: J Clin Microbiol
  doi: 10.1128/JCM.00152-06
– volume: 62
  start-page: 909
  year: 2013
  ident: ppat.1008942.ref016
  article-title: Outbreaks of human metapneumovirus in two skilled nursing facilities -West Virginia and Idaho, 2011–2012
  publication-title: MMWR Morb Mortal Wkly Rep
– volume: 88
  start-page: 2719
  year: 2007
  ident: ppat.1008942.ref059
  article-title: Characterization of the epitope for anti-human respiratory syncytial virus F protein monoclonal antibody 101F using synthetic peptides and genetic approaches
  publication-title: J Gen Virol
  doi: 10.1099/vir.0.82753-0
– volume: 81
  start-page: 8315
  year: 2007
  ident: ppat.1008942.ref049
  article-title: A recombinant human monoclonal antibody to human metapneumovirus fusion protein that neutralizes virus in vitro and is effective therapeutically in vivo
  publication-title: J Virol
  doi: 10.1128/JVI.00106-07
– volume: 40
  start-page: 333
  year: 2007
  ident: ppat.1008942.ref021
  article-title: Co-circulation of human metapneumovirus and SARS-associated coronavirus during a major nosocomial SARS outbreak in Hong Kong
  publication-title: J Clin Virol
  doi: 10.1016/j.jcv.2007.08.015
– volume: 345
  start-page: 492
  year: 2006
  ident: ppat.1008942.ref030
  article-title: Individual contributions of the human metapneumovirus F, G, and SH surface glycoproteins to the induction of neutralizing antibodies and protective immunity
  publication-title: Virology
  doi: 10.1016/j.virol.2005.10.016
– volume: 86
  start-page: 3230
  year: 2012
  ident: ppat.1008942.ref031
  article-title: Human Metapneumovirus (HMPV) binding and infection are mediated by interactions between the HMPV fusion protein and heparan sulfate
  publication-title: J Virol
  doi: 10.1128/JVI.06706-11
– volume: 84
  start-page: 12236
  year: 2010
  ident: ppat.1008942.ref070
  article-title: Structure of a major antigenic site on the respiratory syncytial virus fusion glycoprotein in complex with neutralizing antibody 101F
  publication-title: J Virol
  doi: 10.1128/JVI.01579-10
– volume: 201
  start-page: 297
  year: 2010
  ident: ppat.1008942.ref018
  article-title: Viral pathogens including human metapneumovirus are the primary cause of febrile respiratory illness in HIV-infected adults receiving antiretroviral therapy
  publication-title: J Infect Dis
  doi: 10.1086/649587
– volume: 3
  start-page: 1
  year: 2015
  ident: ppat.1008942.ref056
  article-title: Use of human hybridoma technology to isolate human monoclonal antibodies
  publication-title: Microbiol Spectr
  doi: 10.1128/microbiolspec.AID-0027-2014
– volume: 2
  start-page: 16272
  year: 2017
  ident: ppat.1008942.ref052
  article-title: Structural basis for antibody cross-neutralization of respiratory syncytial virus and human metapneumovirus
  publication-title: Nat Microbiol
  doi: 10.1038/nmicrobiol.2016.272
– volume: 26
  start-page: 479
  year: 2019
  ident: ppat.1008942.ref017
  article-title: Epidemiological survey in a day care center following toddler sudden death due to human metapneumovirus infection
  publication-title: Arch Pediatr
  doi: 10.1016/j.arcped.2019.10.002
– volume: 2
  start-page: 16271
  year: 2017
  ident: ppat.1008942.ref045
  article-title: A novel pre-fusion conformation-specific neutralizing epitope on the respiratory syncytial virus fusion protein
  publication-title: Nat Microbiol
  doi: 10.1038/nmicrobiol.2016.271
– volume: 501
  start-page: 439
  year: 2013
  ident: ppat.1008942.ref051
  article-title: Cross-neutralization of four paramyxoviruses by a human monoclonal antibody
  publication-title: Nature
  doi: 10.1038/nature12442
– volume: 88
  start-page: 6423
  year: 2014
  ident: ppat.1008942.ref027
  article-title: The human metapneumovirus small hydrophobic protein has properties consistent with those of a viroporin and can modulate viral fusogenic activity
  publication-title: J Virol
  doi: 10.1128/JVI.02848-13
– volume: 31
  start-page: 309
  year: 2003
  ident: ppat.1008942.ref009
  article-title: Human metapneumovirus in a haematopoietic stem cell transplant recipient with fatal lower respiratory tract disease
  publication-title: Bone Marrow Transplant
  doi: 10.1038/sj.bmt.1703849
– volume: 340
  start-page: 1113
  year: 2013
  ident: ppat.1008942.ref066
  article-title: Structure of RSV fusion glycoprotein trimer bound to a prefusion-specific neutralizing antibody
  publication-title: Science
  doi: 10.1126/science.1234914
– volume: 15
  start-page: 97
  year: 2013
  ident: ppat.1008942.ref010
  article-title: Fatal human metapneumovirus infection following allogeneic hematopoietic stem cell transplantation
  publication-title: Transpl Infect Dis
  doi: 10.1111/tid.12074
– volume: 82
  start-page: 11767
  year: 2008
  ident: ppat.1008942.ref028
  article-title: Role of cellular glycosaminoglycans and charged regions of viral G protein in human metapneumovirus infection
  publication-title: J Virol
  doi: 10.1128/JVI.01208-08
– volume: 329
  start-page: 112
  year: 2008
  ident: ppat.1008942.ref071
  article-title: Efficient generation of monoclonal antibodies from single human B cells by single cell RT-PCR and expression vector cloning
  publication-title: J Immunol Methods
  doi: 10.1016/j.jim.2007.09.017
– volume: 86
  start-page: 12148
  year: 2012
  ident: ppat.1008942.ref033
  article-title: The human metapneumovirus fusion protein mediates entry via an interaction with RGD-binding integrins
  publication-title: J Virol
  doi: 10.1128/JVI.01133-12
– volume: 211
  start-page: 1
  year: 2014
  ident: ppat.1008942.ref050
  article-title: A broadly neutralizing human monoclonal antibody exhibits in vivo efficacy against both human metapneumovirus and respiratory syncytial virus
  publication-title: J Infect Dis
– volume: 365
  start-page: 505
  year: 2019
  ident: ppat.1008942.ref041
  article-title: A proof of concept for structure-based vaccine design targeting RSV in humans
  publication-title: Science
  doi: 10.1126/science.aav9033
– volume: 1
  start-page: 1
  year: 2016
  ident: ppat.1008942.ref044
  article-title: Rapid profiling of RSV antibody repertoires from the memory B cells of naturally infected adult donors
  publication-title: Sci Immunol
– volume: 17
  start-page: 481
  year: 2010
  ident: ppat.1008942.ref057
  article-title: High Seroprevalence of Neutralizing Capacity against Human Metapneumovirus in All Age Groups Studied in Bonn, Germany
  publication-title: Clin Vaccine Immunol
  doi: 10.1128/CVI.00398-09
– volume: 3
  start-page: 143
  year: 2013
  ident: ppat.1008942.ref068
  article-title: Intermediate conformations during viral fusion glycoprotein structural transition
  publication-title: Curr Opin Virol
  doi: 10.1016/j.coviro.2013.03.006
– volume: 60
  start-page: 2126
  year: 2004
  ident: ppat.1008942.ref075
  article-title: Coot: model-building tools for molecular graphics
  publication-title: Acta Cryst
– volume: 6
  start-page: 8143
  year: 2015
  ident: ppat.1008942.ref040
  article-title: A highly stable prefusion RSV F vaccine derived from structural analysis of the fusion mechanism
  publication-title: Nat Commun
  doi: 10.1038/ncomms9143
– volume: 25
  start-page: 827
  year: 2019
  ident: ppat.1008942.ref061
  article-title: Influenza Antigen Engineering Focuses Immune Responses to a Subdominant but Broadly Protective Viral Epitope
  publication-title: Cell Host Microbe
  doi: 10.1016/j.chom.2019.04.003
– volume: 10
  start-page: 2778
  year: 2019
  ident: ppat.1008942.ref035
  article-title: Antibody Epitopes of Pneumovirus Fusion Proteins
  publication-title: Front Immunol
  doi: 10.3389/fimmu.2019.02778
– volume: 350
  start-page: 2151
  year: 2004
  ident: ppat.1008942.ref025
  article-title: Human metapneumovirus and lower respiratory tract disease in otherwise healthy infants and children
  publication-title: N Engl J Med
  doi: 10.1056/NEJMoa025472
– volume: 215
  start-page: 1536
  year: 2018
  ident: ppat.1008942.ref019
  article-title: Human metapneumovirus infection in chronic obstructive pulmonary disease: Impact of glucocorticosteroids and interferon
  publication-title: J Infect Dis
  doi: 10.1093/infdis/jix167
– volume: 78
  start-page: 12877
  year: 2004
  ident: ppat.1008942.ref036
  article-title: Recombinant human metapneumovirus lacking the small hydrophobic SH and/or attachment G glycoprotein: Deletion of G yields a promising vaccine candidate
  publication-title: J Virol
  doi: 10.1128/JVI.78.23.12877-12887.2004
– volume: 10
  start-page: 658
  year: 2004
  ident: ppat.1008942.ref026
  article-title: Antigenic and genetic variability of human metapneumoviruses
  publication-title: Emerg Infect Dis
  doi: 10.3201/eid1004.030393
– volume: 80
  start-page: 10931
  year: 2006
  ident: ppat.1008942.ref042
  article-title: Characterization of human metapneumovirus F protein-promoted membrane fusion: critical roles for proteolytic processing and low pH
  publication-title: J Virol
  doi: 10.1128/JVI.01287-06
– volume: 6
  start-page: 8167
  year: 2015
  ident: ppat.1008942.ref069
  article-title: Antibodies to a conformational epitope on gp41 neutralize HIV-1 by destabilizing the Env spike
  publication-title: Nat Commun
  doi: 10.1038/ncomms9167
– volume: 103
  start-page: 75
  year: 2020
  ident: ppat.1008942.ref024
  article-title: Co-infection with SARS-CoV-2 and Human Metapneumovirus
  publication-title: R I Med J (2013)
SSID ssj0041316
Score 2.45538
Snippet Human metapneumovirus (hMPV) is a leading cause of viral respiratory infection in children, and can cause severe lower respiratory tract infection in infants,...
SourceID plos
doaj
pubmedcentral
osti
proquest
gale
pubmed
crossref
SourceType Open Website
Open Access Repository
Aggregation Database
Index Database
Enrichment Source
StartPage e1008942
SubjectTerms Antibodies
Antibodies, Monoclonal - immunology
Antibodies, Neutralizing - immunology
Antibodies, Neutralizing - pharmacology
Antibodies, Viral - immunology
Antigens
BASIC BIOLOGICAL SCIENCES
Biology and Life Sciences
Crystallization
Engineering and Technology
Epidemics
Epitopes
Epitopes - immunology
F 2 region
F protein
Fusion protein
Genetic aspects
Glycan
Glycoproteins
Health aspects
Host-virus relationships
Human subjects
Humans
Identification and classification
Immune response
Immune response (humoral)
Immune system
Immunocompromised hosts
Immunology
Infants
Infections
Infectious diseases
Interferometry
Medicine and Health Sciences
Metapneumovirus - immunology
Monoclonal antibodies
Mutation
Neutralization
Neutralizing
Paramyxoviridae Infections - virology
Peptides
Physical Sciences
Pneumovirus - immunology
Proteins
Recognition
Research and Analysis Methods
Respiratory diseases
Respiratory syncytial virus
Respiratory Syncytial Virus, Human - immunology
Respiratory tract
Respiratory tract diseases
RNA viruses
Severe acute respiratory syndrome coronavirus 2
Trimers
Vaccines
Veterinary colleges
Veterinary medicine
Viral Fusion Proteins - immunology
Viral infections
Viral proteins
Viruses
SummonAdditionalLinks – databaseName: DOAJ Directory of Open Access Journals
  dbid: DOA
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Nb9QwELXQSkhcEJSPLm2RQUicQpPYsZ3jgigFiQoBlXqzbMeGlYqz2iRI_ffMJNmoQUW9cF3PfvjN2POcHb8h5JVg2EoyZYnC67m8KIqkzCuXKM9kJS2DpIh3hz-fidNz_umiuLjW6gtrwgZ54AG449SKPHcqDdwC-3DWwlYbKoY65SwY11MjyHm7w9SwB8PO3Dc9xaY4iWRCjJfmmMyORx-92WwMykenquT5LCn12v3TDr2oYamh8ull3dzEQv8upryWnU4ekPsjraSrYToPyR0f98jdodHk1SPyYRXbta2rKzoVDNWR1oEC_aNfou9-1b_X266hocOnZ7RXb1hH2qL4_5aipsQWYPCPyfnJ--_vTpOxhULiZMHbhAfBHKRo7jNnlBDcMV-i6p9QmbGZCMpCQlJFyESqvEUxe29EFmQpSm9LyZ6QRayj3yeUV3D2qRgDD3iuXGGNgaNSyJHxVZURS8J2GGo36otjm4tL3f9pJuGcMWChEXk9Ir8kyfSuzaCvcYv9W3TPZIvq2P0LEDN6jBl9W8wsyUt0rkb9i4gFNj9M1zT647czvRIc622BBf3T6OvM6PVoFGqYrDPjpQaADHW1ZpaHM0tYxW42fICBpoH3oHivwyonB7NWeFE6XZJ9jL8dIo3OuUhLxRjj8Lm7mLx5-MU0jF-JJXXR1x3acNSaYwwwfTqE8IQq_CbsmwA-lbPgnsE-H4nrn704uSxKIDzi2f_w0wG5l-PjDazXKA_Jot12_gg4YGuf98v9D87jVoY
  priority: 102
  providerName: Directory of Open Access Journals
– databaseName: Health & Medical Collection
  dbid: 7X7
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwhV3db9MwELegCIkXBOVjZQMZhMRTWBI7tvOECmIMpE0IGNqbFTv2VmnEpWmQ9t9zl7qBoAGv8bVx7sN3tu9-R8hzwbCVZMoSheW5vCiKpMxrmyjHZC0NA6eItcNHx-LwhH84LU7jgVsb0yq3a2K_UNfB4hn5fs5FWirGGH-1_J5g1yi8XY0tNK6TGwhdhlotT4cNF6zPfetTbI2TSCZELJ1jMtuPknq5XFYIIp2qkucj19Qj-A_r9CSAwSH-6UVor4pF_0yp_M1HHdwht2NwSecbbbhLrrlmSm5u2k1eTsn0K-a-9AW49Cjeqd8j7-bNemFCfUmHZKLQ0OAphIb0Y-O6b-HHYtW11Hd4skZ7ZIdFQ9fYGGBFEW9i5Svr7pOTg7df3hwmsb1CYmXB1wn3gllw39xltlJCcMtciYiAQmWVyYRXBpyVKnwmUuUMAt27SmRelqJ0ppTsAZk0oXE7hPIa9kU1Y8ZkjitbmKqCbZTPMRqs60rMCNtyVtuIPY4tMC50f6EmYQ-y4ZBGeegojxlJhl8tN9gb_6F_jUIbaBE5u38QVmc6GqJOjchzq1LPDUSzFmacM18zxL1nwKxqRp6hyDViYzSYfHNWdW2r338-1nPBMRcXIqS_En0aEb2IRD7Ax9oqFjwAyxBza0S5N6IEC7ej4V1UPw0xEQL7WsyAsvDVCouo0xnZQa3ccqTVvywF_nerqVcPPx2G8ZWYbte40CENRxw6xoCnDzeKPXAV5oQ9FUCmcqTyI7aPR5rFeQ9cLosSgiHx6N_T2iW3cjzUwCyNco9M1qvOPYbIb22e9Ob9E6s3VVE
  priority: 102
  providerName: ProQuest
Title Antibody recognition of the Pneumovirus fusion protein trimer interface
URI https://www.ncbi.nlm.nih.gov/pubmed/33035266
https://www.proquest.com/docview/2460983334
https://www.proquest.com/docview/2449959332
https://www.osti.gov/servlets/purl/1834810
https://pubmed.ncbi.nlm.nih.gov/PMC7598476
https://doaj.org/article/0b622c80f4b045cbb123fd382293faca
http://dx.doi.org/10.1371/journal.ppat.1008942
Volume 16
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV3fa9swEBZdymAvY7-btgveGOzJxbZkSX4YIx3tukFD6RbIm7BkqQ1kdmrHY_nvd-c4Zh4p22t0duxPJ91JPn0fIe84RSnJgPoSj-eyOI79JMqMLy0VmdAUgiKeHb6c8Isp-zqLZ3tkq9naAljtXNqhntS0XJz8ult_hAH_oVFtEOH2opPlMkVC6EAmDCblfYhNAjUNLln3XQFm7EYMFcVyfEE5bw_T3XeXXrBqOP27mXtQwBBERtRFUe3KTv8usvwjap0_IY_bdNMbb_zjKdmz-TPycCNAuX5OPo_z1VwX2drrComK3CucB2mhd5Xb-kfxc17Wledq3FXzGlaHee6tUBSg9JBronSpsS_I9Pzs-6cLv5VW8I2I2cpnjlMDoZvZ0KSSc2aoTZANkMsw1SF3UkOgkrELeSCtRpJ7m_LQiYQnVieCviSDvMjtAfFYBmuijFKtQ8ukiXWawhLKRZgJZlnKh4RuMVSm5R1H-YuFaj6mCVh_bLBQiLxqkR8Sv7tqueHd-If9KXZPZ4us2c0PRXmj2kGoAs2jyMjAMQ2ZrIEnjqjLKHLeUwArHZK32LkKeTFyLLy5SeuqUl--TdSYM6zDhezoXqPrntH71sgV8LImbQ87AGTIt9WzPO5Zwug2veYjdDQF-RCS-hqsfjLw1hIPUAdDcoD-t0WkUhHjQSIppQzuu_XJ3c1vumb8Syy1y21Row1DDjpKAdNXGxfuUIVnQj0F6FPRc-4e7P2WfH7bkJaLOIFEiB_-D3pH5FGE2xpYp5Eck8GqrO1ryP1WekQeiJkYkf3Ts8nV9ajZQRk1Q_w3UyNZDg
linkProvider Scholars Portal
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV3db9MwELdGEYIXBOVjZQMMAvEUlsSO4zwgVD5Gy9YKwYb6ZmLHGZVGUpoG1H-Kv5G7NAkEDXjaa31Jnbuz786--x0hjwTDVpIucySW5_IgCJzIT4wjLQuTUDMwilg7PJmK0TF_OwtmW-RHUwuDaZXNnlht1Elu8Ix8z-fCjSRjjD9ffHWwaxTerjYtNDZqcWDX3yFkK56NX4F8H_v-_uujlyOn7irgmDDgK4enghmwWtx6JpZCcMNshEB4Qnqx9kQqNezRMkg94UqrEd_dxsJLw0hEVkchg_deIBfB8LoY7IWzNsADe1C1WsVWPE7IhKhL9Vjo7dWa8XSxiBG02pUR9zumsOoY0NqFXg4LHPFWT_PiLN_3zxTO32zi_jVytXZm6XCjfdfJls365NKmveW6T_ofMdemKvilk_oO_wZ5M8xWc50na9omL-UZzVMKrih9l9nyS_5tviwLmpZ4kkcrJIl5RlfYiGBJEd9imcbG3iTH58L4W6SX5ZndJpQnEIcljGntWS5NoOMYwrbUR-8zSWIxIKzhrDI11jm23DhV1QVeCDHPhkMK5aFqeQyI0z612GB9_If-BQqtpUWk7uqHfHmi6oWvXC1830g35Rq8ZwMz9lmaMMTZZ8CseEAeosgVYnFkmOxzEpdFocYfpmooOOb-gkf2V6L3HaInNVGaw8eauC6wAJYhxleHcrdDCTuK6QzvoPop8MEQSNhgxpWBr5ZYtO0OyDZqZcORQv1amfDeRlPPHn7QDuNfYnpfZvMSaTji3jEGPL29UeyWqzAn7OEAMg07Kt9he3ckm3-ugNLDIALnS9z597Tuk8ujo8mhOhxPD3bIFR8PVDBDJNolvdWytHfB61zpe9VSp-TTee8tPwFhdZA6
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1fb9MwELdGJxAvCMqflQ0ICMRTaBI7TvKAUMdWVsaqajC0NxM7zqg0ktI0oH41Ph13iRMIGvC01_qSOnfnu7N99ztCnnKKrSQdaodYnst837cjL1F2qGmQBJKCU8Ta4aMpPzhhb0_90w3yo6mFwbTKxiZWhjrJFZ6RDz3GnSiklLJhatIiZnvjV4uvNnaQwpvWpp1GrSKHev0dtm_Fy8keyPqZ5433P7w-sE2HAVsFPlvZLOVUgQdj2lVxyDlTVEcIisdDN5YuT0MJ9jr0U5c7oZaI9a5j7qZBxCMto4DCe6-QzQB3RT2yubs_nR03fgC8Q9V4FRvz2AHl3BTu0cAdGj15sVjECGHthBHzOo6x6h_QeoleDssd0VfP8-KiSPjPhM7fPOT4JrlhQltrVOviLbKhsz65Wje7XPdJ_yNm3lTlv9aRudG_Td6MstVc5snaalOZ8szKUwsCU2uW6fJL_m2-LAsrLfFcz6pwJeaZtcK2BEsL0S6Waaz0HXJyKay_S3pZnuktYrEEdmUJpVK6moXKl3EMm7jUw1g0SWI-ILThrFAG-RwbcJyL6jovgB1QzSGB8hBGHgNit08tauSP_9DvotBaWsTtrn7Il2fCmAHhSO55KnRSJiGWVjBjj6YJRdR9CsyKB-QJilwgMkeGOn4Wl0UhJu-nYsQZZgJDfPZXouMO0XNDlObwsSo25RbAMkT86lDudCjBvqjO8Daqn4CIDGGFFeZfKfjqEEu4nQHZQq1sOFKIX-sU3tto6sXDj9th_EtM9st0XiINQxQ8SoGn92rFbrkKc8KODiDToKPyHbZ3R7L55wo2PfAjCMX4_X9P6xG5BnZFvJtMD7fJdQ9PVzBdJNohvdWy1A8gBF3Jh2atW-TTZZuXnyv8ldU
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Antibody+recognition+of+the+Pneumovirus+fusion+protein+trimer+interface&rft.jtitle=PLoS+pathogens&rft.au=Huang%2C+Jiachen&rft.au=Diaz%2C+Darren&rft.au=Mousa%2C+Jarrod+J&rft.date=2020-10-09&rft.pub=Public+Library+of+Science&rft.issn=1553-7366&rft.volume=16&rft.issue=10&rft_id=info:doi/10.1371%2Fjournal.ppat.1008942&rft.externalDBID=ISR&rft.externalDocID=A645330129
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1553-7374&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1553-7374&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1553-7374&client=summon