Babesia gibsoni internal transcribed spacer 1 region is highly conserved amongst isolates from dogs across Japan

Babesia gibsoni is a tick-borne apicomplexan parasite of dogs that often causes fever and hemolytic anemia with highly variable clinical outcome. In this study, we sequenced the 254bp Internal Transcribed Spacer 1 region (ITS1) of 54 B. gibsoni isolates from 14 different geographical regions of Japa...

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Published inJournal of Veterinary Medical Science Vol. 78; no. 5; pp. 863 - 865
Main Authors LIU, Mingming, CAO, Shinuo, VUDRIKO, Patrick, SUZUKI, Hiroshi, SOMA, Takehisa, XUAN, Xuenan
Format Journal Article
LanguageEnglish
Published Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.01.2016
Japanese Society of Veterinary Science
Japan Science and Technology Agency
The Japanese Society of Veterinary Science
Subjects
Animals
Babesia - genetics
Babesia gibsoni
Babesiosis - epidemiology
Babesiosis - parasitology
conserved gene
Conserved Sequence - genetics
DNA, Intergenic - genetics
Dog Diseases - epidemiology
Dog Diseases - parasitology
Dogs
Genetic Markers - genetics
internal transcribed spacer 1 (ITS1)
Japan
Japan - epidemiology
Parasitology
Phylogeny
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Abstract Babesia gibsoni is a tick-borne apicomplexan parasite of dogs that often causes fever and hemolytic anemia with highly variable clinical outcome. In this study, we sequenced the 254bp Internal Transcribed Spacer 1 region (ITS1) of 54 B. gibsoni isolates from 14 different geographical regions of Japan. The 54 isolates shared high sequence identity with each other and with B. gibsoni isolates reported in GenBank database (97.2–100%). Consistent with previous reports, phylogenetic analysis showed that B. gibsoni isolates from Japan formed the same clade with those from U.S.A., Australia, India and Taiwan. Our finding indicates that B. gibsoni ITS1 region is highly conserved among isolates from dogs in Japan, making it a useful genetic marker for molecular epidemiology of the parasite.
AbstractList Babesia gibsoni is a tick-borne apicomplexan parasite of dogs that often causes fever and hemolytic anemia with highly variable clinical outcome. In this study, we sequenced the 254bp Internal Transcribed Spacer 1 region (ITS1) of 54 B. gibsoni isolates from 14 different geographical regions of Japan. The 54 isolates shared high sequence identity with each other and with B. gibsoni isolates reported in GenBank database (97.2–100%). Consistent with previous reports, phylogenetic analysis showed that B. gibsoni isolates from Japan formed the same clade with those from U.S.A., Australia, India and Taiwan. Our finding indicates that B. gibsoni ITS1 region is highly conserved among isolates from dogs in Japan, making it a useful genetic marker for molecular epidemiology of the parasite.
Babesia gibsoni is a tick-borne apicomplexan parasite of dogs that often causes fever and hemolytic anemia with highly variable clinical outcome. In this study, we sequenced the 254bp Internal Transcribed Spacer 1 region (ITS1) of 54 B. gibsoni isolates from 14 different geographical regions of Japan. The 54 isolates shared high sequence identity with each other and with B. gibsoni isolates reported in GenBank database (97.2-100%). Consistent with previous reports, phylogenetic analysis showed that B. gibsoni isolates from Japan formed the same clade with those from U.S.A., Australia, India and Taiwan. Our finding indicates that B. gibsoni ITS1 region is highly conserved among isolates from dogs in Japan, making it a useful genetic marker for molecular epidemiology of the parasite.Babesia gibsoni is a tick-borne apicomplexan parasite of dogs that often causes fever and hemolytic anemia with highly variable clinical outcome. In this study, we sequenced the 254bp Internal Transcribed Spacer 1 region (ITS1) of 54 B. gibsoni isolates from 14 different geographical regions of Japan. The 54 isolates shared high sequence identity with each other and with B. gibsoni isolates reported in GenBank database (97.2-100%). Consistent with previous reports, phylogenetic analysis showed that B. gibsoni isolates from Japan formed the same clade with those from U.S.A., Australia, India and Taiwan. Our finding indicates that B. gibsoni ITS1 region is highly conserved among isolates from dogs in Japan, making it a useful genetic marker for molecular epidemiology of the parasite.
Babesia gibsoni is a tick-borne apicomplexan parasite of dogs that often causes fever and hemolytic anemia with highly variable clinical outcome. In this study, we sequenced the 254bp Internal Transcribed Spacer 1 region (ITS1) of 54 B. gibsoni isolates from 14 different geographical regions of Japan. The 54 isolates shared high sequence identity with each other and with B. gibsoni isolates reported in GenBank database (97.2–100%). Consistent with previous reports, phylogenetic analysis showed that B. gibsoni isolates from Japan formed the same clade with those from U.S.A., Australia, India and Taiwan. Our finding indicates that B. gibsoni ITS1 region is highly conserved among isolates from dogs in Japan, making it a useful genetic marker for molecular epidemiology of the parasite.
Author SOMA, Takehisa
LIU, Mingming
SUZUKI, Hiroshi
XUAN, Xuenan
VUDRIKO, Patrick
CAO, Shinuo
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Cites_doi 10.1016/j.meegid.2015.01.011
10.1089/vbz.2008.0116
10.1136/vr.142.7.168
10.1093/molbev/msr121
10.1186/1756-3305-4-141
10.1016/j.meegid.2014.07.033
10.1016/j.vetpar.2007.11.024
10.1016/j.vetpar.2004.03.012
10.1007/BF01731581
10.1292/jvms.10-0093
10.1016/S0195-5616(03)00039-1
10.1016/S0020-7519(00)00120-X
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– reference: 5. Hernandez, R., Martinez-Calvillo, S., Hernandez-Rivas, R. and Gomez, E. 1993. Trypanosoma cruzi ribosomal RNA genes: a review. Biol. Res. 26: 109–114.
– reference: 3. Bostrom, B., Wolf, C., Greene, C. and Peterson, D. S. 2008. Sequence conservation in the rRNA first internal transcribed spacer region of Babesia gibsoni genotype Asia isolates. Vet. Parasitol. 152: 152–157.
– reference: 11. Lee, M. J., Yu, D. H., Yoon, J. S., Li, Y. H., Lee, J. H., Chae, J. S. and Park, J. 2009. Epidemiologic and clinical surveys in dogs infected with Babesia gibsoni in South Korea. Vector Borne Zoonotic Dis. 9: 681–686.
– reference: 7. Inokuma, H., Yoshizaki, Y., Matsumoto, K., Okuda, M., Onishi, T., Nakagome, K., Kosugi, R. and Hirakawa, M. 2004. Molecular survey of Babesia infection in dogs in Okinawa, Japan. Vet. Parasitol. 121: 341–346.
– reference: 1. Abd Rani, P. A., Irwin, P. J., Coleman, G. T., Gatne, M. and Traub, R. J. 2011. A survey of canine tick-borne diseases in India. Parasit. Vectors 4: 141.
– reference: 12. Mandal, M., Banerjee, P. S., Garg, R., Ram, H., Kundu, K., Kumar, S. and Kumar, G. V. 2014. Genetic characterization and phylogenetic relationships based on 18S rRNA and ITS1 region of small form of canine Babesia spp. from India. Infect. Genet. Evol. 27: 325–331.
– reference: 16. Terao, M., Akter, S., Yasin, M. G., Nakao, R., Kato, H., Alam, M. Z. and Katakura, K. 2015. Molecular detection and genetic diversity of Babesia gibsoni in dogs in Bangladesh. Infect. Genet. Evol. 31: 53–60.
– reference: 8. Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16: 111–120.
– reference: 9. Kjemtrup, A. M., Kocan, A. A., Whitworth, L., Meinkoth, J., Birkenheuer, A. J., Cummings, J., Boudreaux, M. K., Stockham, S. L., Irizarry-Rovira, A. and Conrad, P. A. 2000. There are at least three genetically distinct small piroplasms from dogs. Int. J. Parasitol. 30: 1501–1505.
– reference: 14. Powers, T. O., Todd, T. C., Burnell, A. M., Murray, P. C., Fleming, C. C., Szalanski, A. L., Adams, B. A. and Harris, T. S. 1997. The rDNA Internal transcribed spacer region as a taxonomic marker for nematodes. J. Nematol. 29: 441–450.
– reference: 4. Casapulla, R., Baldi, L., Avallone, V., Sannino, R., Pazzanese, L. and Mizzoni, V. 1998. Canine piroplasmosis due to Babesia gibsoni: clinical and morphological aspects. Vet. Rec. 142: 168–169.
– reference: 13. Mokhtar, A. S., Lim, S. F. and Tay, S. T. 2013. Molecular detection of Anaplasma platys and Babesia gibsoni in dogs in Malaysia. Trop. Biomed. 30: 345–348.
– reference: 2. Boozer, A. L. and Macintire, D. K. 2003. Canine babesiosis. Vet. Clin. North Am. Small Anim. Pract. 33: 885–904, viii.
– reference: 6. Hřibová, E., Čížková, J., Christelová, P., Taudien, S., de Langhe, E. and Doležel, J. 2011. The ITS1-5.8S-ITS2 sequence region in the Musaceae: structure, diversity and use in molecular phylogeny. PLoS ONE 6: e17863.
– ident: 15
  doi: 10.1016/j.meegid.2015.01.011
– ident: 10
  doi: 10.1089/vbz.2008.0116
– ident: 5
– ident: 4
  doi: 10.1136/vr.142.7.168
– ident: 14
  doi: 10.1093/molbev/msr121
– ident: 1
  doi: 10.1186/1756-3305-4-141
– ident: 12
– ident: 11
  doi: 10.1016/j.meegid.2014.07.033
– ident: 3
  doi: 10.1016/j.vetpar.2007.11.024
– ident: 13
– ident: 6
  doi: 10.1016/j.vetpar.2004.03.012
– ident: 7
  doi: 10.1007/BF01731581
– ident: 9
  doi: 10.1292/jvms.10-0093
– ident: 2
  doi: 10.1016/S0195-5616(03)00039-1
– ident: 8
  doi: 10.1016/S0020-7519(00)00120-X
– reference: 20519852 - J Vet Med Sci. 2010 Oct;72(10):1329-35
– reference: 19274180 - J Nematol. 1997 Dec;29(4):441-50
– reference: 21445344 - PLoS One. 2011 Mar 22;6(3):e17863
– reference: 25620376 - Infect Genet Evol. 2015 Apr;31:53-60
– reference: 11428342 - Int J Parasitol. 2000 Dec;30(14):1501-5
– reference: 19271995 - Vector Borne Zoonotic Dis. 2009 Dec;9(6):681-6
– reference: 15135876 - Vet Parasitol. 2004 May 26;121(3-4):341-6
– reference: 18207327 - Vet Parasitol. 2008 Mar 25;152(1-2):152-7
– reference: 7463489 - J Mol Evol. 1980 Dec;16(2):111-20
– reference: 23959500 - Trop Biomed. 2013 Jun;30(2):345-8
– reference: 21771313 - Parasit Vectors. 2011 Jul 19;4:141
– reference: 12910748 - Vet Clin North Am Small Anim Pract. 2003 Jul;33(4):885-904, viii
– reference: 25120099 - Infect Genet Evol. 2014 Oct;27:325-31
– reference: 9519430 - Vet Rec. 1998 Feb 14;142(7):168-9
– reference: 21546353 - Mol Biol Evol. 2011 Oct;28(10):2731-9
– reference: 7670521 - Biol Res. 1993;26(1-2):109-14
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Snippet Babesia gibsoni is a tick-borne apicomplexan parasite of dogs that often causes fever and hemolytic anemia with highly variable clinical outcome. In this...
Babesia gibsoni is a tick-borne apicomplexan parasite of dogs that often causes fever and hemolytic anemia with highly variable clinical outcome. In this...
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SubjectTerms Animals
Babesia - genetics
Babesia gibsoni
Babesiosis - epidemiology
Babesiosis - parasitology
conserved gene
Conserved Sequence - genetics
DNA, Intergenic - genetics
Dog Diseases - epidemiology
Dog Diseases - parasitology
Dogs
Genetic Markers - genetics
internal transcribed spacer 1 (ITS1)
Japan
Japan - epidemiology
Parasitology
Phylogeny
Title Babesia gibsoni internal transcribed spacer 1 region is highly conserved amongst isolates from dogs across Japan
URI https://www.jstage.jst.go.jp/article/jvms/78/5/78_15-0483/_article/-char/en
https://cir.nii.ac.jp/crid/1572824502624533504
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https://www.proquest.com/docview/1793901965
https://www.proquest.com/docview/1808627931
https://pubmed.ncbi.nlm.nih.gov/PMC4905845
Volume 78
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ispartofPNX Journal of Veterinary Medical Science, 2016, Vol.78(5), pp.863-865
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