Coronavirus cell entry occurs through the endo-/lysosomal pathway in a proteolysis-dependent manner

• Published in: PLoS pathogens Vol. 10; no. 11; p. e1004502
• Main Authors: Burkard, Christine, Verheije, Monique H, Wicht, Oliver, van Kasteren, Sander I, van Kuppeveld, Frank J, Haagmans, Bart L, Pelkmans, Lucas, Rottier, Peter J M, Bosch, Berend Jan, de Haan, Cornelis A M
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.11.2014; Public Library of Science (PLoS)
• Subjects: Animals; Bacteriology; Biology and life sciences; Cats; Cercopithecus aethiops; Colleges & universities; Coronaviruses; Dogs; Endosomes - metabolism; Endosomes - virology; Experiments; Genes; Genomes; Health aspects; HeLa Cells; Hepatitis; HIV; Host-virus relationships; Human immunodeficiency virus; Humans; Infections; Influenza; Lysosomes - metabolism; Lysosomes - virology; Madin Darby Canine Kidney Cells; Medicine and Health Sciences; Membrane Fusion; Mice; Microscopy; Murine hepatitis virus - genetics; Murine hepatitis virus - metabolism; Peptides; Proteins; Proteolysis; Spike Glycoprotein, Coronavirus - genetics; Spike Glycoprotein, Coronavirus - metabolism; Vero Cells; Virus Internalization; Virus research; Viruses; Zoonoses

Enveloped viruses need to fuse with a host cell membrane in order to deliver their genome into the host cell. While some viruses fuse with the plasma membrane, many viruses are endocytosed prior to fusion. Specific cues in the endosomal microenvironment induce conformational changes in the viral fus...