Rapid and sensitive detection of elephant endotheliotropic herpesvirus 1 (EEHV1) in blood by loop-mediated isothermal amplification (LAMP)

• Published in: Journal of Veterinary Medical Science Vol. 81; no. 3; pp. 504 - 507
• Main Authors: TAKEHANA, Kazuya, KINJYO, Teruo, NEMOTO, Manabu, MATSUNO, Keita
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2019; Japan Science and Technology Agency; The Japanese Society of Veterinary Science
• Subjects: acute hemorrhagic disease; Animals; Asian elephants (Elephas maximus); DNA Primers; DNA-Directed DNA Polymerase - genetics; elephant endotheliotropic herpesvirus (EEHV); Elephants - blood; Elephants - virology; Female; Hemorrhage; Hemorrhagic disease; Herpesviridae - classification; Herpesviridae - enzymology; Herpesviridae - genetics; Herpesviridae - isolation & purification; Herpesviridae Infections - diagnosis; Herpesviridae Infections - veterinary; Herpesviridae Infections - virology; loop-mediated isothermal amplification (LAMP); Male; Nucleic Acid Amplification Techniques - methods; Nucleic Acid Amplification Techniques - veterinary; Plasmids; polymerase chain reaction (PCR); Polymerase Chain Reaction - veterinary; Sensitivity and Specificity; Time Factors; Wildlife Science; Asian elephants (Elephas maximus); elephant endotheliotropic herpesvirus (EEHV); acute hemorrhagic disease; polymerase chain reaction (PCR); loop-mediated isothermal amplification (LAMP)

Elephant endotheliotropic herpesvirus type 1 (EEHV1) is the most important causative agent of an acute fatal hemorrhagic disease in Asian elephants (Elephas maximus). We employed loop-mediated isothermal amplification (LAMP) to develop a rapid and simple detection method for EEHV1 in blood. When use...