Rapid and sensitive detection of elephant endotheliotropic herpesvirus 1 (EEHV1) in blood by loop-mediated isothermal amplification (LAMP)
Elephant endotheliotropic herpesvirus type 1 (EEHV1) is the most important causative agent of an acute fatal hemorrhagic disease in Asian elephants (Elephas maximus). We employed loop-mediated isothermal amplification (LAMP) to develop a rapid and simple detection method for EEHV1 in blood. When use...
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| Published in | Journal of Veterinary Medical Science Vol. 81; no. 3; pp. 504 - 507 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
Japan
JAPANESE SOCIETY OF VETERINARY SCIENCE
2019
Japan Science and Technology Agency The Japanese Society of Veterinary Science |
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| Abstract | Elephant endotheliotropic herpesvirus type 1 (EEHV1) is the most important causative agent of an acute fatal hemorrhagic disease in Asian elephants (Elephas maximus). We employed loop-mediated isothermal amplification (LAMP) to develop a rapid and simple detection method for EEHV1 in blood. When used to test 21 clinical samples collected in Japan, the EEHV1 assay correctly identified one positive and 20 negative clinical samples. It was observed that when samples were spiked with synthetic DNA plasmids including EEHV1 polymerase gene, the detection limit of the LAMP assay was 101.2 copies/µl and 100-fold higher than that of conventional PCR. These advantages of the LAMP assay for EEHV1 detection may facilitate better veterinary practices for treating elephants suffering from the acute disease. |
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| AbstractList | Elephant endotheliotropic herpesvirus type 1 (EEHV1) is the most important causative agent of an acute fatal hemorrhagic disease in Asian elephants (Elephas maximus). We employed loop-mediated isothermal amplification (LAMP) to develop a rapid and simple detection method for EEHV1 in blood. When used to test 21 clinical samples collected in Japan, the EEHV1 assay correctly identified one positive and 20 negative clinical samples. It was observed that when samples were spiked with synthetic DNA plasmids including EEHV1 polymerase gene, the detection limit of the LAMP assay was 101.2 copies/µl and 100-fold higher than that of conventional PCR. These advantages of the LAMP assay for EEHV1 detection may facilitate better veterinary practices for treating elephants suffering from the acute disease. Elephant endotheliotropic herpesvirus type 1 (EEHV1) is the most important causative agent of an acute fatal hemorrhagic disease in Asian elephants (Elephas maximus). We employed loop-mediated isothermal amplification (LAMP) to develop a rapid and simple detection method for EEHV1 in blood. When used to test 21 clinical samples collected in Japan, the EEHV1 assay correctly identified one positive and 20 negative clinical samples. It was observed that when samples were spiked with synthetic DNA plasmids including EEHV1 polymerase gene, the detection limit of the LAMP assay was 10 copies/µl and 100-fold higher than that of conventional PCR. These advantages of the LAMP assay for EEHV1 detection may facilitate better veterinary practices for treating elephants suffering from the acute disease. Elephant endotheliotropic herpesvirus type 1 (EEHV1) is the most important causative agent of an acute fatal hemorrhagic disease in Asian elephants ( Elephas maximus ). We employed loop-mediated isothermal amplification (LAMP) to develop a rapid and simple detection method for EEHV1 in blood. When used to test 21 clinical samples collected in Japan, the EEHV1 assay correctly identified one positive and 20 negative clinical samples. It was observed that when samples were spiked with synthetic DNA plasmids including EEHV1 polymerase gene, the detection limit of the LAMP assay was 10 1.2 copies/ µl and 100-fold higher than that of conventional PCR. These advantages of the LAMP assay for EEHV1 detection may facilitate better veterinary practices for treating elephants suffering from the acute disease. Elephant endotheliotropic herpesvirus type 1 (EEHV1) is the most important causative agent of an acute fatal hemorrhagic disease in Asian elephants (Elephas maximus). We employed loop-mediated isothermal amplification (LAMP) to develop a rapid and simple detection method for EEHV1 in blood. When used to test 21 clinical samples collected in Japan, the EEHV1 assay correctly identified one positive and 20 negative clinical samples. It was observed that when samples were spiked with synthetic DNA plasmids including EEHV1 polymerase gene, the detection limit of the LAMP assay was 101.2 copies/µl and 100-fold higher than that of conventional PCR. These advantages of the LAMP assay for EEHV1 detection may facilitate better veterinary practices for treating elephants suffering from the acute disease.Elephant endotheliotropic herpesvirus type 1 (EEHV1) is the most important causative agent of an acute fatal hemorrhagic disease in Asian elephants (Elephas maximus). We employed loop-mediated isothermal amplification (LAMP) to develop a rapid and simple detection method for EEHV1 in blood. When used to test 21 clinical samples collected in Japan, the EEHV1 assay correctly identified one positive and 20 negative clinical samples. It was observed that when samples were spiked with synthetic DNA plasmids including EEHV1 polymerase gene, the detection limit of the LAMP assay was 101.2 copies/µl and 100-fold higher than that of conventional PCR. These advantages of the LAMP assay for EEHV1 detection may facilitate better veterinary practices for treating elephants suffering from the acute disease. |
| Author | NEMOTO, Manabu MATSUNO, Keita TAKEHANA, Kazuya KINJYO, Teruo |
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| Keywords | Asian elephants (Elephas maximus) elephant endotheliotropic herpesvirus (EEHV) acute hemorrhagic disease polymerase chain reaction (PCR) loop-mediated isothermal amplification (LAMP) |
| Language | English |
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| References | 7. Long, S. Y., Latimer, E. M. and Hayward, G. S. 2016. Review of elephant endotheliotropic herpesviruses and acute hemorrhagic disease. ILAR J. 56: 283–296. 9. Parida, M., Sannarangaiah, S., Dash, P. K., Rao, P. V. L. and Morita, K. 2008. Loop mediated isothermal amplification (LAMP): a new generation of innovative gene amplification technique; perspectives in clinical diagnosis of infectious diseases. Rev. Med. Virol. 18: 407–421. 2. Dastjerdi, A., Seilern-Moy, K., Darpel, K., Steinbach, F. and Molenaar, F. 2016. Surviving and fatal Elephant Endotheliotropic Herpesvirus-1A infections in juvenile Asian elephants - lessons learned and recommendations on anti-herpesviral therapy. BMC Vet. Res. 12: 178. 5. Kendall, R., Howard, L., Masters, N. and Grant, R. 2016. The impact of elephant endotheliotropic herpesvirus on the captive Asian elephant (Elephas maximus) population of the United Kingdom and Ireland (1995–2013). J. Zoo Wildl. Med. 47: 405–418. 11. Richman, L. K. and Hayward, G. S. 2012. Elephant herpesviruses. pp. 496–502. In: Fowler’s Zoo and Wild Animal Medicine Current Therapy Volume 7 (Miller, R. E. and Fowler, M. eds.), Elsevier Health Science, Amsterdam. 21. Wilkie, G. S., Davison, A. J., Watson, M., Kerr, K., Sanderson, S., Bouts, T., Steinbach, F. and Dastjerdi, A. 2013. Complete genome sequences of elephant endotheliotropic herpesviruses 1A and 1B determined directly from fatal cases. J. Virol. 87: 6700–6712. 1. Azab, W., Damiani, A. M., Ochs, A. and Osterrieder, N. 2018. Subclinical infection of a young captive Asian elephant with elephant endotheliotropic herpesvirus 1. Arch. Virol. 163: 495–500. 19. van den Doel, P. B., Prieto, V. R., van Rossum-Fikkert, S. E., Schaftenaar, W., Latimer, E., Howard, L., Chapman, S., Masters, N., Osterhaus, A. D. M. E., Ling, P. D., Dastjerdi, A. and Martina, B. 2015. A novel antigen capture ELISA for the specific detection of IgG antibodies to elephant endotheliotropic herpes virus. BMC Vet. Res. 11: 203. 20. Whelan, J. A., Russell, N. B. and Whelan, M. A. 2003. A method for the absolute quantification of cDNA using real-time PCR. J. Immunol. Methods 278: 261–269. 12. Richman, L. K., Montali, R. J., Garber, R. L., Kennedy, M. A., Lehnhardt, J., Hildebrandt, T., Schmitt, D., Hardy, D., Alcendor, D. J. and Hayward, G. S. 1999. Novel endotheliotropic herpesviruses fatal for Asian and African elephants. Science 283: 1171–1176. 3. Fuery, A., Tan, J., Peng, R., Flanagan, J. P., Tocidlowski, M. E., Howard, L. L. and Ling, P. D. 2016. Clinical infection of two captive Asian elephants (Elephas maximus) with elephant endotheliotropic herpesvirus 1B. J. Zoo Wildl. Med. 47: 319–324. 15. Sripiboon, S., Angkawanish, T., Boonprasert, K., Sombutputorn, P., Langkaphin, W., Ditcham, W. and Warren, K. 2017. Successful treatment of a clinical elephant endotheliotropic herpesvirus infection: The dynamics of viral load, genotype analysis, and treatment with acyclovir. J. Zoo Wildl. Med. 48: 1254–1259. 6. Latimer, E., Zong, J. C., Heaggans, S. Y., Richman, L. K. and Hayward, G. S. 2011. Detection and evaluation of novel herpesviruses in routine and pathological samples from Asian and African elephants: identification of two new probosciviruses (EEHV5 and EEHV6) and two new gammaherpesviruses (EGHV3B and EGHV5). Vet. Microbiol. 147: 28–41. 8. Notomi, T., Okayama, H., Masubuchi, H., Yonekawa, T., Watanabe, K., Amino, N. and Hase, T. 2000. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 28: E63. 14. Schaftenaar, W., Reid, C., Martina, B., Fickel, J. and Osterhaus, A. D. M. E. 2010. Nonfatal clinical presentation of elephant endotheliotropic herpes virus discovered in a group of captive Asian elephants (Elephas maximus). J. Zoo Wildl. Med. 41: 626–632. 16. Stanton, J. J., Cray, C., Rodriguez, M., Arheart, K. L., Ling, P. D. and Herron, A. 2013. Acute phase protein expression during elephant endotheliotropic herpesvirus-1 viremia in Asian elephants (Elephas maximus). J. Zoo Wildl. Med. 44: 605–612. 17. Stanton, J. J., Zong, J. C., Eng, C., Howard, L., Flanagan, J., Stevens, M., Schmitt, D., Wiedner, E., Graham, D., Junge, R. E., Weber, M. A., Fischer, M., Mejia, A., Tan, J., Latimer, E., Herron, A., Hayward, G. S. and Ling, P. D. 2013. Kinetics of viral loads and genotypic analysis of elephant endotheliotropic herpesvirus-1 infection in captive Asian elephants (Elephas maximus). J. Zoo Wildl. Med. 44: 42–54. 10. Reed, L. J. and Muench, H. 1938. A simple method of estimating fifty per cent endpoints. Am. J. Hyg. 27: 493–497. 13. Sahoo, P. R., Sethy, K., Mohapatra, S. and Panda, D. 2016. Loop mediated isothermal amplification: An innovative gene amplification technique for animal diseases. Vet. World 9: 465–469. 4. Howard, L. L. and Schaftenaar, W. 2018. Elephant endotheliotropic herpesvirus. pp. 672–679. In: Miller-Fowler’s Zoo and Wild Animal Medicine Current Therapy, Volume 9 (Miller, R. E., Lamberski, N. and Calle, P. eds.), Elsevier Health Sciences, Amsterdam. 18. Taniguchi, M., Oba, M., Okazaki, S., Teshima, Y., Shiina, O., Kinjou, T., Azama, K., Shimabukuro, Y., Fukuhara, M., Nakamoto, H., Sochi, C., Kiryu, Y., Miyake, I., Fujino, M., Masatou, H., Iida, S., Shinohara, H., Oouchi, I., Takahara, K., Kimura, S., Sassa, Y., Nagai, M., Furuya, T., Kawakami, S. and Mizutani, T. 2014. Epidemiology of Elephant endotheliotropic Herpesvirus 1 in Elephants in Japanese Zoos. Jour. Jpn. Assoc. Zoo. Aqu. 55: 41–44. 11 12 13 14 15 16 17 18 19 1 2 3 4 5 6 7 8 9 20 10 21 |
| References_xml | – reference: 9. Parida, M., Sannarangaiah, S., Dash, P. K., Rao, P. V. L. and Morita, K. 2008. Loop mediated isothermal amplification (LAMP): a new generation of innovative gene amplification technique; perspectives in clinical diagnosis of infectious diseases. Rev. Med. Virol. 18: 407–421. – reference: 5. Kendall, R., Howard, L., Masters, N. and Grant, R. 2016. The impact of elephant endotheliotropic herpesvirus on the captive Asian elephant (Elephas maximus) population of the United Kingdom and Ireland (1995–2013). J. Zoo Wildl. Med. 47: 405–418. – reference: 8. Notomi, T., Okayama, H., Masubuchi, H., Yonekawa, T., Watanabe, K., Amino, N. and Hase, T. 2000. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 28: E63. – reference: 16. Stanton, J. J., Cray, C., Rodriguez, M., Arheart, K. L., Ling, P. D. and Herron, A. 2013. Acute phase protein expression during elephant endotheliotropic herpesvirus-1 viremia in Asian elephants (Elephas maximus). J. Zoo Wildl. Med. 44: 605–612. – reference: 2. Dastjerdi, A., Seilern-Moy, K., Darpel, K., Steinbach, F. and Molenaar, F. 2016. Surviving and fatal Elephant Endotheliotropic Herpesvirus-1A infections in juvenile Asian elephants - lessons learned and recommendations on anti-herpesviral therapy. BMC Vet. Res. 12: 178. – reference: 1. Azab, W., Damiani, A. M., Ochs, A. and Osterrieder, N. 2018. Subclinical infection of a young captive Asian elephant with elephant endotheliotropic herpesvirus 1. Arch. Virol. 163: 495–500. – reference: 3. Fuery, A., Tan, J., Peng, R., Flanagan, J. P., Tocidlowski, M. E., Howard, L. L. and Ling, P. D. 2016. Clinical infection of two captive Asian elephants (Elephas maximus) with elephant endotheliotropic herpesvirus 1B. J. Zoo Wildl. Med. 47: 319–324. – reference: 6. Latimer, E., Zong, J. C., Heaggans, S. Y., Richman, L. K. and Hayward, G. S. 2011. Detection and evaluation of novel herpesviruses in routine and pathological samples from Asian and African elephants: identification of two new probosciviruses (EEHV5 and EEHV6) and two new gammaherpesviruses (EGHV3B and EGHV5). Vet. Microbiol. 147: 28–41. – reference: 20. Whelan, J. A., Russell, N. B. and Whelan, M. A. 2003. A method for the absolute quantification of cDNA using real-time PCR. J. Immunol. Methods 278: 261–269. – reference: 13. Sahoo, P. R., Sethy, K., Mohapatra, S. and Panda, D. 2016. Loop mediated isothermal amplification: An innovative gene amplification technique for animal diseases. Vet. World 9: 465–469. – reference: 18. Taniguchi, M., Oba, M., Okazaki, S., Teshima, Y., Shiina, O., Kinjou, T., Azama, K., Shimabukuro, Y., Fukuhara, M., Nakamoto, H., Sochi, C., Kiryu, Y., Miyake, I., Fujino, M., Masatou, H., Iida, S., Shinohara, H., Oouchi, I., Takahara, K., Kimura, S., Sassa, Y., Nagai, M., Furuya, T., Kawakami, S. and Mizutani, T. 2014. Epidemiology of Elephant endotheliotropic Herpesvirus 1 in Elephants in Japanese Zoos. Jour. Jpn. Assoc. Zoo. Aqu. 55: 41–44. – reference: 4. Howard, L. L. and Schaftenaar, W. 2018. Elephant endotheliotropic herpesvirus. pp. 672–679. In: Miller-Fowler’s Zoo and Wild Animal Medicine Current Therapy, Volume 9 (Miller, R. E., Lamberski, N. and Calle, P. eds.), Elsevier Health Sciences, Amsterdam. – reference: 7. Long, S. Y., Latimer, E. M. and Hayward, G. S. 2016. Review of elephant endotheliotropic herpesviruses and acute hemorrhagic disease. ILAR J. 56: 283–296. – reference: 17. Stanton, J. J., Zong, J. C., Eng, C., Howard, L., Flanagan, J., Stevens, M., Schmitt, D., Wiedner, E., Graham, D., Junge, R. E., Weber, M. A., Fischer, M., Mejia, A., Tan, J., Latimer, E., Herron, A., Hayward, G. S. and Ling, P. D. 2013. Kinetics of viral loads and genotypic analysis of elephant endotheliotropic herpesvirus-1 infection in captive Asian elephants (Elephas maximus). J. Zoo Wildl. Med. 44: 42–54. – reference: 10. Reed, L. J. and Muench, H. 1938. A simple method of estimating fifty per cent endpoints. Am. J. Hyg. 27: 493–497. – reference: 11. Richman, L. K. and Hayward, G. S. 2012. Elephant herpesviruses. pp. 496–502. In: Fowler’s Zoo and Wild Animal Medicine Current Therapy Volume 7 (Miller, R. E. and Fowler, M. eds.), Elsevier Health Science, Amsterdam. – reference: 21. Wilkie, G. S., Davison, A. J., Watson, M., Kerr, K., Sanderson, S., Bouts, T., Steinbach, F. and Dastjerdi, A. 2013. Complete genome sequences of elephant endotheliotropic herpesviruses 1A and 1B determined directly from fatal cases. J. Virol. 87: 6700–6712. – reference: 12. Richman, L. K., Montali, R. J., Garber, R. L., Kennedy, M. A., Lehnhardt, J., Hildebrandt, T., Schmitt, D., Hardy, D., Alcendor, D. J. and Hayward, G. S. 1999. Novel endotheliotropic herpesviruses fatal for Asian and African elephants. Science 283: 1171–1176. – reference: 19. van den Doel, P. B., Prieto, V. R., van Rossum-Fikkert, S. E., Schaftenaar, W., Latimer, E., Howard, L., Chapman, S., Masters, N., Osterhaus, A. D. M. E., Ling, P. D., Dastjerdi, A. and Martina, B. 2015. A novel antigen capture ELISA for the specific detection of IgG antibodies to elephant endotheliotropic herpes virus. BMC Vet. Res. 11: 203. – reference: 14. Schaftenaar, W., Reid, C., Martina, B., Fickel, J. and Osterhaus, A. D. M. E. 2010. Nonfatal clinical presentation of elephant endotheliotropic herpes virus discovered in a group of captive Asian elephants (Elephas maximus). J. Zoo Wildl. Med. 41: 626–632. – reference: 15. Sripiboon, S., Angkawanish, T., Boonprasert, K., Sombutputorn, P., Langkaphin, W., Ditcham, W. and Warren, K. 2017. Successful treatment of a clinical elephant endotheliotropic herpesvirus infection: The dynamics of viral load, genotype analysis, and treatment with acyclovir. J. Zoo Wildl. Med. 48: 1254–1259. – ident: 7 doi: 10.1093/ilar/ilv041 – ident: 18 – ident: 20 doi: 10.1016/S0022-1759(03)00223-0 – ident: 2 doi: 10.1186/s12917-016-0806-5 – ident: 17 doi: 10.1638/1042-7260-44.1.42 – ident: 21 doi: 10.1128/JVI.00655-13 – ident: 4 doi: 10.1016/B978-0-323-55228-8.00095-3 – ident: 13 doi: 10.14202/vetworld.2016.465-469 – ident: 1 doi: 10.1007/s00705-017-3628-2 – ident: 14 doi: 10.1638/2009-0217.1 – ident: 16 doi: 10.1638/2012-0174R1.1 – ident: 3 doi: 10.1638/2015-0074.1 – ident: 5 doi: 10.1638/2015-0217.1 – ident: 19 doi: 10.1186/s12917-015-0522-6 – ident: 10 doi: 10.1093/oxfordjournals.aje.a118408 – ident: 8 doi: 10.1093/nar/28.12.e63 – ident: 12 doi: 10.1126/science.283.5405.1171 – ident: 11 doi: 10.1016/B978-1-4377-1986-4.00065-2 – ident: 9 doi: 10.1002/rmv.593 – ident: 6 doi: 10.1016/j.vetmic.2010.05.042 – ident: 15 doi: 10.1638/2016-0141R1.1 |
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| Snippet | Elephant endotheliotropic herpesvirus type 1 (EEHV1) is the most important causative agent of an acute fatal hemorrhagic disease in Asian elephants (Elephas... Elephant endotheliotropic herpesvirus type 1 (EEHV1) is the most important causative agent of an acute fatal hemorrhagic disease in Asian elephants ( Elephas... |
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| SubjectTerms | acute hemorrhagic disease Animals Asian elephants (Elephas maximus) DNA Primers DNA-Directed DNA Polymerase - genetics elephant endotheliotropic herpesvirus (EEHV) Elephants - blood Elephants - virology Female Hemorrhage Hemorrhagic disease Herpesviridae - classification Herpesviridae - enzymology Herpesviridae - genetics Herpesviridae - isolation & purification Herpesviridae Infections - diagnosis Herpesviridae Infections - veterinary Herpesviridae Infections - virology loop-mediated isothermal amplification (LAMP) Male Nucleic Acid Amplification Techniques - methods Nucleic Acid Amplification Techniques - veterinary Plasmids polymerase chain reaction (PCR) Polymerase Chain Reaction - veterinary Sensitivity and Specificity Time Factors Wildlife Science |
| Title | Rapid and sensitive detection of elephant endotheliotropic herpesvirus 1 (EEHV1) in blood by loop-mediated isothermal amplification (LAMP) |
| URI | https://www.jstage.jst.go.jp/article/jvms/81/3/81_18-0683/_article/-char/en https://www.ncbi.nlm.nih.gov/pubmed/30700652 https://www.proquest.com/docview/2239621765 https://www.proquest.com/docview/2179451137 https://pubmed.ncbi.nlm.nih.gov/PMC6451909 |
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| ispartofPNX | Journal of Veterinary Medical Science, 2019, Vol.81(3), pp.504-507 |
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