The use of high pressure CO 2 ‐facilitated pH swings to enhance in situ product recovery of butyric acid in a two‐phase partitioning bioreactor
ABSTRACT Through the use of high partial pressures of CO 2 (pCO 2 ) to facilitate temporary pH reductions in two‐phase partitioning bioreactors (TPPBs), improved pH dependent partitioning of butyric acid was observed which achieved in situ product recovery (ISPR), alleviating end‐product inhibition...
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Published in | Biotechnology and bioengineering Vol. 111; no. 11; pp. 2183 - 2191 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
01.11.2014
|
Online Access | Get full text |
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Abstract | ABSTRACT
Through the use of high partial pressures of CO
2
(pCO
2
) to facilitate temporary pH reductions in two‐phase partitioning bioreactors (TPPBs), improved pH dependent partitioning of butyric acid was observed which achieved in situ product recovery (ISPR), alleviating end‐product inhibition (EPI) during the production of butyric acid by
Clostridium tyrobutyricum
(ATCC 25755). Through high pressure pCO
2
studies, media buffering effects were shown to be substantially overcome at 60 bar pCO
2
, resulting in effective extraction of the organic acid by the absorptive polymer Pebax® 2533, yielding a distribution coefficient (D) of 2.4 ± 0.1 after 1 h of contact at this pressure. Importantly, it was also found that
C. tyrobutyricum
cultures were able to withstand 60 bar pCO
2
for 1 h with no decrease in growth ability when returned to atmospheric pressure in batch reactors after several extraction cycles. A fed‐batch reactor with cyclic high pCO
2
polymer extraction recovered 92 g of butyric acid to produce a total of 213 g compared to 121 g generated in a control reactor. This recovery reduced EPI in the TPPB, resulting in both higher productivity (0.65 vs. 0.33 g L
−1
h
−1
) and yield (0.54 vs. 0.40). Fortuitously, it was also found that repeated high pCO
2
‐facilitated polymer extractions of butyric acid during batch growth of
C. tyrobutyricum
lessened the need for pH control, and reduced base requirements by approximately 50%. Thus, high pCO
2
‐mediated absorptive polymer extraction presents a novel method for improving process performance in butyric acid fermentation, and this technique could be applied to the bioproduction of other organic acids as well. Biotechnol. Bioeng. 2014;111: 2183–2191. © 2014 Wiley Periodicals, Inc. |
---|---|
AbstractList | ABSTRACT
Through the use of high partial pressures of CO
2
(pCO
2
) to facilitate temporary pH reductions in two‐phase partitioning bioreactors (TPPBs), improved pH dependent partitioning of butyric acid was observed which achieved in situ product recovery (ISPR), alleviating end‐product inhibition (EPI) during the production of butyric acid by
Clostridium tyrobutyricum
(ATCC 25755). Through high pressure pCO
2
studies, media buffering effects were shown to be substantially overcome at 60 bar pCO
2
, resulting in effective extraction of the organic acid by the absorptive polymer Pebax® 2533, yielding a distribution coefficient (D) of 2.4 ± 0.1 after 1 h of contact at this pressure. Importantly, it was also found that
C. tyrobutyricum
cultures were able to withstand 60 bar pCO
2
for 1 h with no decrease in growth ability when returned to atmospheric pressure in batch reactors after several extraction cycles. A fed‐batch reactor with cyclic high pCO
2
polymer extraction recovered 92 g of butyric acid to produce a total of 213 g compared to 121 g generated in a control reactor. This recovery reduced EPI in the TPPB, resulting in both higher productivity (0.65 vs. 0.33 g L
−1
h
−1
) and yield (0.54 vs. 0.40). Fortuitously, it was also found that repeated high pCO
2
‐facilitated polymer extractions of butyric acid during batch growth of
C. tyrobutyricum
lessened the need for pH control, and reduced base requirements by approximately 50%. Thus, high pCO
2
‐mediated absorptive polymer extraction presents a novel method for improving process performance in butyric acid fermentation, and this technique could be applied to the bioproduction of other organic acids as well. Biotechnol. Bioeng. 2014;111: 2183–2191. © 2014 Wiley Periodicals, Inc. |
Author | Daugulis, Andrew J. Peterson, Eric C. |
Author_xml | – sequence: 1 givenname: Eric C. surname: Peterson fullname: Peterson, Eric C. organization: Department of Chemical Engineering Queen's University Dupuis Hall 19 Division St. Kingston Ontario Canada K7L3N6 – sequence: 2 givenname: Andrew J. surname: Daugulis fullname: Daugulis, Andrew J. organization: Department of Chemical Engineering Queen's University Dupuis Hall 19 Division St. Kingston Ontario Canada K7L3N6 |
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CitedBy_id | crossref_primary_10_1016_j_biotechadv_2018_09_005 crossref_primary_10_1016_j_fluid_2017_03_005 crossref_primary_10_1002_bit_27550 crossref_primary_10_1186_s13068_018_1120_1 crossref_primary_10_1016_j_cej_2015_11_068 crossref_primary_10_1002_btpr_2148 crossref_primary_10_1021_acs_jafc_3c01356 |
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Snippet | ABSTRACT
Through the use of high partial pressures of CO
2
(pCO
2
) to facilitate temporary pH reductions in two‐phase partitioning bioreactors (TPPBs),... |
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Title | The use of high pressure CO 2 ‐facilitated pH swings to enhance in situ product recovery of butyric acid in a two‐phase partitioning bioreactor |
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