Langerhans cell markers CD 1a and CD 207 are the most rapidly responding genes in lesional psoriatic skin following adalimumab treatment

Abstract TNF α‐, IL ‐23‐ and IL ‐17‐targeting drugs are highly effective in the treatment of psoriasis. However, the precise molecular mechanism remains unknown. In psoriatic skin, the presence of Langerhans cells ( LC s) is reduced, but the role of LC is poorly understood. The purpose of this study...

Full description

Saved in:
Bibliographic Details
Published inExperimental dermatology Vol. 26; no. 9; pp. 804 - 810
Main Authors Raaby, Line, Rosada, Cecilia, Langkilde, Ane, Lauridsen, Kristina Lystlund, Vinter, Hanne, Ommen, Pernille, Kjellerup, Rasmus Boye, Johansen, Claus, Iversen, Lars
Format Journal Article
LanguageEnglish
Published 01.09.2017
Online AccessGet full text

Cover

Abstract Abstract TNF α‐, IL ‐23‐ and IL ‐17‐targeting drugs are highly effective in the treatment of psoriasis. However, the precise molecular mechanism remains unknown. In psoriatic skin, the presence of Langerhans cells ( LC s) is reduced, but the role of LC is poorly understood. The purpose of this study was to investigate the impact of TNF α and IL ‐23/ IL ‐17 on the presence of LC in the skin during treatment. Therefore, psoriatic skin was investigated before and after 4 days of adalimumab or ustekinumab treatment. Furthermore, TNF α and IL ‐17A stimulation was investigated in an ex vivo model of epidermis and dermis from healthy normal skin kept in cultures at an air‐liquid interphase for 4 days. In a gene array analysis, we found that the two LC markers, CD 1a and CD 207, were among the most up‐ or downregulated genes in psoriatic skin after anti‐ TNF α therapy. Validation showed that both mRNA expression and protein level followed the same pattern and became significantly upregulated after 4 days of treatment. No changes were seen after ustekinumab treatment. In the ex vivo skin model, a decrease in the CD 1a level was seen after TNF α stimulation and it was caused by LC migration from epidermis. No response in LC migration was seen after IL ‐17A stimulation. Taken together, we demonstrated that changes in the LC level in epidermis precede the histological and clinical changes during adalimumab treatment in psoriatic skin. Furthermore, TNF α plays a prominent role in orchestrating LC migration in the skin. This seems not to be the true for the IL ‐23/ IL ‐17A pathway.
AbstractList Abstract TNF α‐, IL ‐23‐ and IL ‐17‐targeting drugs are highly effective in the treatment of psoriasis. However, the precise molecular mechanism remains unknown. In psoriatic skin, the presence of Langerhans cells ( LC s) is reduced, but the role of LC is poorly understood. The purpose of this study was to investigate the impact of TNF α and IL ‐23/ IL ‐17 on the presence of LC in the skin during treatment. Therefore, psoriatic skin was investigated before and after 4 days of adalimumab or ustekinumab treatment. Furthermore, TNF α and IL ‐17A stimulation was investigated in an ex vivo model of epidermis and dermis from healthy normal skin kept in cultures at an air‐liquid interphase for 4 days. In a gene array analysis, we found that the two LC markers, CD 1a and CD 207, were among the most up‐ or downregulated genes in psoriatic skin after anti‐ TNF α therapy. Validation showed that both mRNA expression and protein level followed the same pattern and became significantly upregulated after 4 days of treatment. No changes were seen after ustekinumab treatment. In the ex vivo skin model, a decrease in the CD 1a level was seen after TNF α stimulation and it was caused by LC migration from epidermis. No response in LC migration was seen after IL ‐17A stimulation. Taken together, we demonstrated that changes in the LC level in epidermis precede the histological and clinical changes during adalimumab treatment in psoriatic skin. Furthermore, TNF α plays a prominent role in orchestrating LC migration in the skin. This seems not to be the true for the IL ‐23/ IL ‐17A pathway.
Author Iversen, Lars
Lauridsen, Kristina Lystlund
Johansen, Claus
Raaby, Line
Vinter, Hanne
Kjellerup, Rasmus Boye
Ommen, Pernille
Langkilde, Ane
Rosada, Cecilia
Author_xml – sequence: 1
  givenname: Line
  orcidid: 0000-0002-3327-9613
  surname: Raaby
  fullname: Raaby, Line
  organization: Department of Dermatology Aarhus University Hospital Aarhus Denmark, Department of Pathology Aarhus University Hospital Aarhus Denmark
– sequence: 2
  givenname: Cecilia
  surname: Rosada
  fullname: Rosada, Cecilia
  organization: Department of Dermatology Aarhus University Hospital Aarhus Denmark
– sequence: 3
  givenname: Ane
  surname: Langkilde
  fullname: Langkilde, Ane
  organization: Department of Dermatology Aarhus University Hospital Aarhus Denmark
– sequence: 4
  givenname: Kristina Lystlund
  surname: Lauridsen
  fullname: Lauridsen, Kristina Lystlund
  organization: Department of Pathology Aarhus University Hospital Aarhus Denmark
– sequence: 5
  givenname: Hanne
  surname: Vinter
  fullname: Vinter, Hanne
  organization: Department of Pathology Aarhus University Hospital Aarhus Denmark
– sequence: 6
  givenname: Pernille
  surname: Ommen
  fullname: Ommen, Pernille
  organization: Department of Dermatology Aarhus University Hospital Aarhus Denmark
– sequence: 7
  givenname: Rasmus Boye
  surname: Kjellerup
  fullname: Kjellerup, Rasmus Boye
  organization: Department of Dermatology Aarhus University Hospital Aarhus Denmark
– sequence: 8
  givenname: Claus
  surname: Johansen
  fullname: Johansen, Claus
  organization: Department of Dermatology Aarhus University Hospital Aarhus Denmark
– sequence: 9
  givenname: Lars
  surname: Iversen
  fullname: Iversen, Lars
  organization: Department of Dermatology Aarhus University Hospital Aarhus Denmark
BookMark eNotkM1OwzAQhC0EEm3hwBv4yiFlnR87OaLyK1Xi0nu0jdetqWNHdhD0DXhsUmAuMxqNVtpvzs598MTYjYClmHRHX3opigLKMzYTEiADmVfnbAYNyEwqqC7ZPKV3AKEKVc3Y9xr9juIefeIdOcd7jAeKia8euECOXp9SDopjJD7uifchjTziYLU78khpCF5bv-M78pS49dxRssGj40MK0eJoO54OU2-Cc-HzNEWNzvYfPW75GAnHnvx4xS4MukTX_75gm6fHzeolW789v67u11mnyjKTKDV0WgnSUJY1FTUqaHISJlc1GTJARI2paoWN1I3E3JiKgArcCmUkFAt2-3e2iyGlSKYdop1-PrYC2hPBdiLY_hIsfgBxkmfa
CitedBy_id crossref_primary_10_1111_exd_14549
crossref_primary_10_3390_jpm12122072
crossref_primary_10_14814_phy2_15604
crossref_primary_10_1111_bjd_16596
crossref_primary_10_3389_fimmu_2018_00300
crossref_primary_10_1093_ced_llae006
crossref_primary_10_2147_CCID_S412162
crossref_primary_10_1016_j_phrs_2019_104473
crossref_primary_10_1016_j_jid_2021_01_005
crossref_primary_10_3389_fimmu_2018_00093
crossref_primary_10_1111_exd_13942
Cites_doi 10.2340/00015555-1440
10.1016/j.ejcb.2014.12.003
10.1084/jem.161.3.526
10.1038/nri2455
10.1038/sj.jid.5700799
10.1038/jid.2010.340
10.1111/j.1365-2230.1986.tb00514.x
10.1111/j.1365-2133.1988.tb02432.x
10.1056/NEJMra0804595
10.1016/j.jaci.2009.08.046
10.1084/jem.20052367
10.5114/ceji.2012.30803
10.1159/000232307
10.15252/emmm.201404114
10.1038/ni852
10.1016/0003-9969(94)00152-2
10.1016/j.jss.2014.02.027
10.1073/pnas.1110076108
10.1111/bjd.13969
10.1046/j.1365-2249.2003.02146.x
10.1111/j.1365-2133.2010.10036.x
10.1016/j.jaci.2007.03.006
10.1111/bjd.12705
10.1016/j.cyto.2005.07.011
10.1016/S0140-6736(14)61909-7
10.1007/s00403-002-0334-y
10.1111/j.1365-2133.2005.06816.x
10.1016/j.cyto.2014.03.003
10.1038/jid.2013.289
10.1038/jid.2014.98
10.1046/j.1365-2133.1999.02964.x
ContentType Journal Article
DBID AAYXX
CITATION
DOI 10.1111/exd.13304
DatabaseName CrossRef
DatabaseTitle CrossRef
DatabaseTitleList CrossRef
DeliveryMethod fulltext_linktorsrc
Discipline Medicine
EISSN 1600-0625
EndPage 810
ExternalDocumentID 10_1111_exd_13304
GroupedDBID ---
.3N
.GA
.Y3
05W
0R~
10A
1OB
1OC
29G
31~
33P
36B
3SF
4.4
50Y
50Z
51W
51X
52M
52N
52O
52P
52R
52S
52T
52U
52V
52W
52X
53G
5GY
5HH
5LA
5VS
66C
702
7PT
8-0
8-1
8-3
8-4
8-5
8UM
930
A01
A03
AAESR
AAEVG
AAHHS
AAKAS
AANLZ
AAONW
AASGY
AAXRX
AAYXX
AAZKR
ABCQN
ABCUV
ABDBF
ABEML
ABJNI
ABPVW
ABQWH
ABXGK
ACAHQ
ACBWZ
ACCFJ
ACCZN
ACGFO
ACGFS
ACGOF
ACMXC
ACPOU
ACPRK
ACSCC
ACXBN
ACXQS
ADBBV
ADBTR
ADEOM
ADIZJ
ADKYN
ADMGS
ADOZA
ADXAS
ADZCM
ADZMN
ADZOD
AEEZP
AEGXH
AEIGN
AEIMD
AENEX
AEQDE
AEUQT
AEUYR
AFBPY
AFEBI
AFFPM
AFGKR
AFPWT
AFZJQ
AHBTC
AHEFC
AIACR
AIAGR
AITYG
AIURR
AIWBW
AJBDE
ALAGY
ALMA_UNASSIGNED_HOLDINGS
ALUQN
AMBMR
AMYDB
ASPBG
ATUGU
AVWKF
AZBYB
AZFZN
AZVAB
BAFTC
BDRZF
BFHJK
BHBCM
BMXJE
BROTX
BRXPI
BY8
C45
CAG
CITATION
COF
CS3
CYRXZ
D-6
D-7
D-E
D-F
DC6
DCZOG
DPXWK
DR2
DRFUL
DRMAN
DRSTM
DU5
EAD
EAP
EBC
EBD
EBS
EJD
EMB
EMK
EMOBN
ESX
EX3
F00
F01
F04
F5P
FEDTE
FUBAC
FZ0
G-S
G.N
GODZA
H.X
HF~
HGLYW
HVGLF
HZI
HZ~
IHE
IX1
J0M
K48
KBYEO
LATKE
LC2
LC3
LEEKS
LH4
LITHE
LOXES
LP6
LP7
LUTES
LW6
LYRES
MEWTI
MK4
MRFUL
MRMAN
MRSTM
MSFUL
MSMAN
MSSTM
MXFUL
MXMAN
MXSTM
N04
N05
N9A
NF~
O66
O9-
OIG
OVD
P2P
P2W
P2X
P2Z
P4B
P4D
PALCI
Q.N
Q11
QB0
R.K
RIWAO
RJQFR
ROL
RX1
SAMSI
SUPJJ
SV3
TEORI
TUS
UB1
V8K
W8V
W99
WBKPD
WHWMO
WIH
WIJ
WIK
WOHZO
WOW
WQJ
WRC
WUP
WVDHM
WXI
WXSBR
XG1
YFH
YUY
ZZTAW
~IA
~WT
ID FETCH-LOGICAL-c744-6a6d0cd71ed0448e38a7092e1f278efef0eee9f587a96d96a2ff5e0e3ab17f603
ISSN 0906-6705
IngestDate Fri Aug 23 00:33:19 EDT 2024
IsPeerReviewed true
IsScholarly true
Issue 9
Language English
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-c744-6a6d0cd71ed0448e38a7092e1f278efef0eee9f587a96d96a2ff5e0e3ab17f603
ORCID 0000-0002-3327-9613
PageCount 7
ParticipantIDs crossref_primary_10_1111_exd_13304
PublicationCentury 2000
PublicationDate 2017-09-00
PublicationDateYYYYMMDD 2017-09-01
PublicationDate_xml – month: 09
  year: 2017
  text: 2017-09-00
PublicationDecade 2010
PublicationTitle Experimental dermatology
PublicationYear 2017
References e_1_2_8_28_1
e_1_2_8_29_1
e_1_2_8_24_1
e_1_2_8_25_1
e_1_2_8_26_1
e_1_2_8_27_1
e_1_2_8_3_1
e_1_2_8_2_1
e_1_2_8_5_1
e_1_2_8_4_1
e_1_2_8_7_1
e_1_2_8_6_1
e_1_2_8_9_1
e_1_2_8_8_1
e_1_2_8_20_1
e_1_2_8_21_1
e_1_2_8_22_1
e_1_2_8_23_1
e_1_2_8_17_1
e_1_2_8_18_1
e_1_2_8_19_1
e_1_2_8_13_1
e_1_2_8_14_1
e_1_2_8_15_1
e_1_2_8_16_1
e_1_2_8_32_1
e_1_2_8_10_1
e_1_2_8_31_1
e_1_2_8_11_1
e_1_2_8_12_1
e_1_2_8_30_1
References_xml – ident: e_1_2_8_24_1
  doi: 10.2340/00015555-1440
– ident: e_1_2_8_31_1
  doi: 10.1016/j.ejcb.2014.12.003
– ident: e_1_2_8_3_1
  doi: 10.1084/jem.161.3.526
– ident: e_1_2_8_4_1
  doi: 10.1038/nri2455
– ident: e_1_2_8_27_1
  doi: 10.1038/sj.jid.5700799
– ident: e_1_2_8_22_1
  doi: 10.1038/jid.2010.340
– ident: e_1_2_8_10_1
  doi: 10.1111/j.1365-2230.1986.tb00514.x
– ident: e_1_2_8_26_1
  doi: 10.1111/j.1365-2133.1988.tb02432.x
– ident: e_1_2_8_2_1
  doi: 10.1056/NEJMra0804595
– ident: e_1_2_8_20_1
  doi: 10.1016/j.jaci.2009.08.046
– ident: e_1_2_8_14_1
  doi: 10.1084/jem.20052367
– ident: e_1_2_8_11_1
  doi: 10.5114/ceji.2012.30803
– ident: e_1_2_8_18_1
  doi: 10.1159/000232307
– ident: e_1_2_8_12_1
  doi: 10.15252/emmm.201404114
– ident: e_1_2_8_7_1
  doi: 10.1038/ni852
– ident: e_1_2_8_32_1
  doi: 10.1016/0003-9969(94)00152-2
– ident: e_1_2_8_29_1
  doi: 10.1016/j.jss.2014.02.027
– ident: e_1_2_8_6_1
  doi: 10.1073/pnas.1110076108
– ident: e_1_2_8_28_1
  doi: 10.1111/bjd.13969
– ident: e_1_2_8_17_1
  doi: 10.1046/j.1365-2249.2003.02146.x
– ident: e_1_2_8_25_1
  doi: 10.1111/j.1365-2133.2010.10036.x
– ident: e_1_2_8_8_1
  doi: 10.1016/j.jaci.2007.03.006
– ident: e_1_2_8_21_1
  doi: 10.1111/bjd.12705
– ident: e_1_2_8_5_1
  doi: 10.1016/j.cyto.2005.07.011
– ident: e_1_2_8_19_1
  doi: 10.1016/S0140-6736(14)61909-7
– ident: e_1_2_8_13_1
  doi: 10.1007/s00403-002-0334-y
– ident: e_1_2_8_9_1
  doi: 10.1111/j.1365-2133.2005.06816.x
– ident: e_1_2_8_30_1
  doi: 10.1016/j.cyto.2014.03.003
– ident: e_1_2_8_15_1
  doi: 10.1038/jid.2013.289
– ident: e_1_2_8_23_1
  doi: 10.1038/jid.2014.98
– ident: e_1_2_8_16_1
  doi: 10.1046/j.1365-2133.1999.02964.x
SSID ssj0017375
Score 2.2112432
Snippet Abstract TNF α‐, IL ‐23‐ and IL ‐17‐targeting drugs are highly effective in the treatment of psoriasis. However, the precise molecular mechanism remains...
SourceID crossref
SourceType Aggregation Database
StartPage 804
Title Langerhans cell markers CD 1a and CD 207 are the most rapidly responding genes in lesional psoriatic skin following adalimumab treatment
Volume 26
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3battAEF3cBEpfSq80vbGUvgkFXayV9OjYDaE4fQgu5M2stLOtiCwbSaZNv6B_1N_rjFa39AJpX-RFeGXkOczMzp4zy9hbn_LQJPIRvElgTxOd2nEKvh1qDULoJA0kCZzPP4izj9P3l8HlZPJjxFra18lx-u2PupL_sSreQ7uSSvYfLNs_FG_gGO2LV7QwXm9l42Uj2v2M0caiAry1Ia5NWVnzheU2WisaeU5oEb2LMszNtqqtUu4ylV9bZUOPbUQtn8jjUekjB9Olw9pV2zJrurlWVxmREfN8-6URNCrM3Df7jUwGkvqN8v74yABFfr--Ubm_kNI081yOdvQvthU-tynaQprlWR8r6A2vstz0Bp4NE0jOnam2emQcVSGt5XVV5_tCjUsZGB47rlZfk3SELULHbHOD8ciCpO_CqKM7l21E9i0045H_jcxZxl0oN4zZv0QJ-KqOXSrnDKGw2_7_JUL2vMVuxYRT183UO-zQQw-HrvVwdrI4Oe03sEK_6fHcv1Hb1IpIZP3vjlKhUU6zesDut4sRPjPIesgmUDxid89busVj9n0AGCeA8RZgfL7gruQIMBohwDgCjCPAOAGMtwDjA8B4AzCeFbwDGO8BxglgvAcYHwDGe4A9YavTd6v5md0e3WGn4XRqCymUk6rQBeXg-h_8SIZO7IGrvTACDdoBgFgHUShjoWIhPa0DcMCXiRtq4fhP2UGxLeAZ45BIL3Z9cPw0nVIyT3vBgJ8aAu2l6oi96f7E9c40aFn_Zqbnt_nSC3ZvAOVLdlCXe3iFGWedvG6t-xN1-Ikp
link.rule.ids 315,783,787,27936,27937
linkProvider EBSCOhost
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Langerhans+cell+markers+CD+1a+and+CD+207+are+the+most+rapidly+responding+genes+in+lesional+psoriatic+skin+following+adalimumab+treatment&rft.jtitle=Experimental+dermatology&rft.au=Raaby%2C+Line&rft.au=Rosada%2C+Cecilia&rft.au=Langkilde%2C+Ane&rft.au=Lauridsen%2C+Kristina+Lystlund&rft.date=2017-09-01&rft.issn=0906-6705&rft.eissn=1600-0625&rft.volume=26&rft.issue=9&rft.spage=804&rft.epage=810&rft_id=info:doi/10.1111%2Fexd.13304&rft.externalDBID=n%2Fa&rft.externalDocID=10_1111_exd_13304
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0906-6705&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0906-6705&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0906-6705&client=summon