Identification of candidate mi RNA s and expression profile of yak oocytes before and after in vitro maturation by high‐throughput sequencing
Contents Small RNA represents several unique non‐coding RNA classes that have important function in a wide range of biological processes including development of germ cells and early embryonic, cell differentiation, cell proliferation and apoptosis in diverse organisms. However, little is known abou...
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Published in | Reproduction in domestic animals Vol. 51; no. 6; pp. 886 - 894 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
01.12.2016
|
Online Access | Get full text |
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Summary: | Contents
Small
RNA
represents several unique non‐coding
RNA
classes that have important function in a wide range of biological processes including development of germ cells and early embryonic, cell differentiation, cell proliferation and apoptosis in diverse organisms. However, little is known about their expression profiles and effects in yak oocytes maturation and early development. To investigate the function of small
RNA
s in the maturation process of yak oocyte and early development, two small
RNA
libraries of oocytes were constructed from germinal vesicle stage (
GV
) and maturation in vitro to metaphase
II
‐arrested stage (M
II
) and then sequenced using small
RNA
high‐throughput sequencing technology. A total of 9,742,592 and 12,168,523 clean reads were obtained from
GV
and M
II
oocytes, respectively. In total, 801 and 1,018 known mi
RNA
s were acquired from
GV
and M
II
oocytes, and 75 mi
RNA
s were found to be significantly differentially expressed: 47 mi
RNA
s were upregulated and 28 mi
RNA
s were downregulated in the M
II
oocytes compared to the
GV
stage. Among the upregulated mi
RNA
s, miR‐342 has the largest fold change (9.25‐fold). Six highly expressed mi
RNA
s (let‐7i, miR‐10b, miR‐10c, miR‐143, miR‐146b and miR‐148) were validated by real‐time quantitative
PCR
(
RT
‐
qPCR
) and consistent with the sequencing results. Furthermore, the expression patterns of two mi
RNA
s and their potential targets were analysed in different developmental stages of oocytes and early embryos. This study provides the first mi
RNA
profile in the mature process of yak oocyte. Seventy‐five mi
RNA
s are expressed differentially in
GV
and M
II
oocytes as well as among different development stages of early embryos, suggesting mi
RNA
s involved in regulating oocyte maturation and early development of yak. These results showed specific mi
RNA
s in yak oocytes had dynamic changes during meiosis. Further functional and mechanistic studies on the mi
RNA
s during meiosis may beneficial to understanding the role of mi
RNA
s on meiotic division. |
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ISSN: | 0936-6768 1439-0531 |
DOI: | 10.1111/rda.12754 |