Establishment and directed differentiation of induced pluripotent stem cells from glycogen storage disease type I b patient

• Published in: Genes to cells : devoted to molecular & cellular mechanisms Vol. 18; no. 12; pp. 1053 - 1069
• Main Authors: Satoh, Daisuke, Maeda, Tohru, Ito, Tetsuya, Nakajima, Yoko, Ohte, Mariko, Ukai, Akane, Nakamura, Katsunori, Enosawa, Shin, Toyota, Masashi, Miyagawa, Yoshitaka, Okita, Hajime, Kiyokawa, Nobutaka, Akutsu, Hidenori, Umezawa, Akihiro, Matsunaga, Tamihide
• Format: Journal Article
• Language: English
• Published: 01.12.2013
• ISSN: 1356-9597; 1365-2443
• DOI: 10.1111/gtc.12101

Glycogen storage disease type I b ( GSDI b) is caused by a deficiency in the glucose‐6‐phosphate transporter ( G 6 PT ), which leads to neutrophil dysfunction. However, the underlying causes of these dysfunctions and their relationship with glucose homeostasis are unclear. Induced pluripotent stem cells (i PSC s) hold a great promise for advances in developmental biology, cell‐based therapy and modeling of human disease. Here, we examined the use of i PSC s as a model for GSDI b. In this study, one 2‐year‐old patient was genetically screened and diagnosed with GSDI b. We established i PSC s and differentiated these cells into hepatocytes and neutrophils, which comprise the main pathological components of GSDI b. Cells that differentiated into hepatocytes exhibited characteristic albumin secretion and indocyanine green uptake. Moreover, i PSC ‐derived cells generated from patients with GSDI b metabolic abnormalities recapitulated key pathological features of the diseases affecting the patients from whom they were derived, such as glycogen, lactate, pyruvate and lipid accumulation. Cells that were differentiated into neutrophils also showed the GSDI b pathology. In addition to the expression of neutrophil markers, we showed increased superoxide anion production, increased annexin V binding and activation of caspase‐3 and caspase‐9, consistent with the GSDI b patient's neutrophils. These results indicate valuable tools for the analysis of this pathology and the development of future treatments.