A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing - Mok, Beverly Y.; de Moraes, Marcos H.; Zeng, Jun; Bosch, Dustin E.; Kotrys, Anna V.; Raguram, Aditya; Hsu, FoSheng; Radey, Matthew C.; Peterson, S. Brook; Mootha, Vamsi K.; Mougous, Joseph D.; Liu, David R. (Nature (London), 2020)
Keywords: 45/41; 45/70; 631/1647/1511; 631/326/1320; 631/61/201/2110; 631/80/642/333; Bacterial toxins; Bacterial Toxins - chemistry; Bacterial Toxins - genetics; Bacterial Toxins - metabolism; Base Sequence; Biocompatibility; Biomedical materials; Burkholderia cenocepacia - enzymology; Burkholderia cenocepacia - genetics; Cell Respiration - genetics; Chemical properties; CRISPR; Crystal structure; Cytidine - metabolism; Cytidine deaminase; Cytidine Deaminase - chemistry; Cytidine Deaminase - genetics; Cytidine Deaminase - metabolism; Cytosine; Deamination; Deoxyribonucleic acid; DNA; DNA, Mitochondrial - genetics; DNA-binding protein; E coli; Enzymes; Gene Editing - methods; Genes, Mitochondrial - genetics; Genetic aspects; Genetic modification; Genome editing; Genome, Mitochondrial - genetics; Genomes; HEK293 Cells; Humanities and Social Sciences; Humans; Methods; Microbial enzymes; Mitochondria; Mitochondria - genetics; Mitochondrial Diseases - genetics; Mitochondrial Diseases - therapy; Mitochondrial DNA; multidisciplinary; Mutation; Nuclease; Oxidative Phosphorylation; Phosphorylation; Protein Engineering; Proteins; Ribonucleic acid; RNA; RNA, Guide, CRISPR-Cas Systems - genetics; Science; Science (multidisciplinary); Substrate Specificity; Therapeutic applications; Toxins; Transcription; Type VI Secretion Systems - metabolism; Unwinding; Uracil; United States