Doxorubicin Induces Apoptosis by Activation of Caspase-3 in Cultured Cardiomyocytes In Vitro and Rat Cardiac Ventricles In Vivo

• Published in: Journal of Pharmacological Sciences Vol. 101; no. 2; pp. 151 - 158
• Main Authors: Ueno, Michihiko, Kakinuma, Yoshihiko, Yuhki, Koh-ichi, Murakoshi, Nobuyuki, Iemitsu, Motoyuki, Miyauchi, Takashi, Yamaguchi, Iwao
• Format: Journal Article
• Language: English
• Published: Japan The Japanese Pharmacological Society 2006; Elsevier
• Subjects: Animals; Animals, Newborn; apoptosis; Apoptosis - drug effects; Atrial Natriuretic Factor - metabolism; cardiomyopathy; caspase; Caspase 3; Caspases - metabolism; Cell Culture Techniques; Cells, Cultured; doxorubicin; Doxorubicin - administration & dosage; Doxorubicin - pharmacology; Drug Administration Schedule; Echocardiography; Endothelin-1 - metabolism; Enzyme Activation; Heart Ventricles - cytology; Injections, Intravenous; Male; mitochondria; Myocytes, Cardiac - cytology; Myocytes, Cardiac - drug effects; Myocytes, Cardiac - enzymology; Rats; Rats, Sprague-Dawley; RNA, Messenger - metabolism

Doxorubicin (DOX) is widely used to treat patients suffering from cancer, but the usage for patients is limited because of the dose-dependent cardiotoxicity. We hypothesized that DOX induces apoptosis through caspase activation in cardiomyocytes, and we examined this hypothesis using both rat primar...