Accumulation of Phosphorylated Repressor for Gibberellin Signaling in an F-Box Mutant

• Published in: Science (American Association for the Advancement of Science) Vol. 299; no. 5614; pp. 1896 - 1898
• Main Authors: Sasaki, Akie, Itoh, Hironori, Gomi, Kenji, Ueguchi-Tanaka, Miyako, Ishiyama, Kanako, Kobayashi, Masatomo, Jeong, Dong-Hoon, An, Gynheung, Kitano, Hidemi, Ashikari, Motoyuki, Matsuoka, Makoto
• Format: Journal Article
• Language: English
• Published: Washington, DC American Association for the Advancement of Science 21.03.2003; The American Association for the Advancement of Science
• Subjects: Alleles; alpha-Amylases - biosynthesis; Amino Acid Sequence; Antibodies; Biological and medical sciences; Codon, Terminator; Company growth; DELLA protein; Enzyme Induction; F box proteins; Freshwater; Fundamental and applied biological sciences. Psychology; Genes, Plant; Genetic aspects; Genetics; Gibberellins - metabolism; Gibberellins - pharmacology; GID2 gene; Growth; Immunoblotting; Ligases - metabolism; Literary Devices; Logical Thinking; Molecular Sequence Data; Mutation; Open Reading Frames; Oryza - genetics; Oryza - growth & development; Oryza - metabolism; Oryza sativa; Peptide Hydrolases - metabolism; Phenotype; Phenotypes; Phosphorylation; Plant cells; Plant growth; Plant growth regulators; Plant growth. Development of the storage organs; Plant hormones; Plant physiology and development; Plant proteins; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - metabolism; Plants; Plants (Organisms); Proteasome Endopeptidase Complex; proteasomes; Protein Structure, Tertiary; Rice; Seeds - metabolism; Signal Transduction; SLR1 gene; Ubiquitin - metabolism; Ubiquitin-Protein Ligases; Vegetative apparatus, growth and morphogenesis. Senescence; Yeasts; Japan; Multicatalytic endopeptidase complex; Monocotyledones; Phosphorylation; Enzyme; Growth; Gibberellin; Cereal crop; Protein; Characterization; Dwarfism; Peptidases; Oryza sativa; Signal transduction; Regulation(control); Gene; Gramineae; Angiospermae; Hydrolases; Plant growth substance; Spermatophyta; Mutation
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.1081077

Gibberellin (GA) regulates growth and development in plants. We isolated and characterized a rice GA-insensitive dwarf mutant, gid2. The CID2 gene encodes a putative F-box protein, which interacted with the rice Skp1 homolog in a yeast two-hybrid assay. In gid2, a repressor for GA signaling, SLR1, was highly accumulated in a phosphorylated form and GA increased its concentration, whereas SLR1 was rapidly degraded by GA through ubiquitination in the wild type. We conclude that GID2 is a positive regulator of GA signaling and that regulated degradation of SLR1 is initiated through GA-dependent phosphorylation and finalized by an$SCF^{GID2}-proteasome$pathway.