Predictable and precise template-free CRISPR editing of pathogenic variants - Shen, Max W.; Arbab, Mandana; Hsu, Jonathan Y.; Worstell, Daniel; Culbertson, Sannie J.; Krabbe, Olga; Cassa, Christopher A.; Liu, David R.; Gifford, David K.; Sherwood, Richard I. (Nature (London), 2018)
Keywords: 45; 45/77; 631/114/2785; 631/208/191; Algorithms; Alleles; Artificial intelligence; Base Sequence; Cell lines; CRISPR; CRISPR-Associated Protein 9 - metabolism; CRISPR-Cas Systems - genetics; CRISPR-Cas technology; Data mining; Deoxyribonucleic acid; Design; Disruption; DNA; DNA repair; DNA Repair - genetics; Editing; Fibroblasts; Fibroblasts - metabolism; Fibroblasts - pathology; Gene disruption; Gene Editing - methods; Gene Editing - standards; Genes; Genome editing; Genomes; Genomics; Genotype & phenotype; Genotypes; HCT116 Cells; HEK293 Cells; Hermanski-Pudlak Syndrome - genetics; Hermanski-Pudlak Syndrome - pathology; Hermansky-Pudlak syndrome; Human genetic engineering; Human genome; Humanities and Social Sciences; Humans; Innovations; K562 Cells; Learning algorithms; Libraries; Machine Learning; Medical research; Menkes Kinky Hair Syndrome - genetics; Menkes Kinky Hair Syndrome - pathology; Menkes syndrome; Methods; multidisciplinary; Mutation; Repair; Reproducibility of Results; Science; Science (multidisciplinary); Stem cell research; Stem cells; Substrate Specificity; Templates, Genetic; United States; Microhomology-mediated End Joining (MMEJ); Low-density Lipoprotein Receptor (LDLR); gRNA Expression Plasmid; Hermansky-Pudlak Syndrome (HPS1); Production Editor