Cardiomyocytes cultured on mechanically compliant substrates, but not on conventional culture devices, exhibit prominent mitochondrial dysfunction due to reactive oxygen species and insulin resistance under high glucose

• Published in: PloS one Vol. 13; no. 8; p. e0201891
• Main Authors: Morishima, Masaki, Horikawa, Kazuki, Funaki, Makoto
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 23.08.2018; Public Library of Science (PLoS)
• Subjects: Accumulation; Animals; Animals, Newborn; Apoptosis; Attenuation; Biology and Life Sciences; Biomechanical Phenomena; Blood glucose; Cardiomyocytes; Cardiovascular disease; Caspase; Caspase-3; Cell culture; Cell Culture Techniques - instrumentation; Cells, Cultured; Culture Media; Cytoskeleton; Cytoskeleton - metabolism; Cytoskeleton - pathology; Deoxyribonucleic acid; Diabetes; Diabetes mellitus; Disease control; DNA; DNA fragmentation; Elasticity; Engineering and Technology; Fibroblasts; Gels; Glucose; Glucose - metabolism; Health aspects; Heart; Heart cells; Heart Ventricles; Hyperglycemia; In vivo methods and tests; Insulin; Insulin resistance; Insulin Resistance - physiology; Levels; Mechanical properties; Medicine and Health Sciences; Membrane potential; Microenvironments; Mitochondria; Mitochondria - metabolism; Mitochondrial diseases; Myocytes, Cardiac - metabolism; Myocytes, Cardiac - pathology; Neonates; Oxygen; Physical Sciences; Rats, Wistar; Reactive oxygen species; Reactive Oxygen Species - metabolism; Risk factors; Rodents; Signal transduction; Stiffness; Substrates; Japan

Diabetes causes cardiac dysfunction, and understanding of its mechanism is still incomplete. One reason could be limitations in modeling disease conditions by current in vitro cardiomyocyte culture. Emerging evidence suggests that the mechanical properties of the microenvironment affect cardiomyocyt...