Trace Amounts of Furan-2-Carboxylic Acids Determine the Quality of Solid Agar Plates for Bacterial Culture

• Published in: PloS one Vol. 7; no. 7; p. e41142
• Main Authors: Hara, Shintaro, Isoda, Reika, Tahvanainen, Teemu, Hashidoko, Yasuyuki
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 27.07.2012; Public Library of Science (PLoS)
• Subjects: Agar; Agar - chemistry; Bacteria; Bioassays; Biology; Carboxylic acids; Cell Proliferation; Chemistry; Chromatography; Colonies; Culture Media - chemistry; E coli; Equivalence; Escherichia coli; Escherichia coli K12 - growth & development; Furans; Furans - chemistry; Furoic acid; Motility; Organic acids; Plates; Polysaccharides; Pseudomonas; Quality Control; Quantitative analysis; Salmonella; Salmonella Typhimurium; Swarming; Swimming; Japan

Many investigators have recognised that a significant proportion of environmental bacteria exist in a viable but non-culturable state on agar plates, and some researchers have also noticed that some of such bacteria clearly recover their growth on matrices other than agar. However, the reason why ag...