Impaired Tethering and Fusion of GLUT4 Vesicles in Insulin-Resistant Human Adipose Cells

• Published in: Diabetes (New York, N.Y.) Vol. 62; no. 9; pp. 3114 - 3119
• Main Authors: Lizunov, Vladimir A., Lee, Jo-Ping, Skarulis, Monica C., Zimmerberg, Joshua, Cushman, Samuel W., Stenkula, Karin G.
• Format: Journal Article
• Language: English
• Published: Alexandria, VA American Diabetes Association 01.09.2013
• Subjects: Adipocytes - drug effects; Adipocytes - metabolism; Adipose tissue; Adipose tissues; Biological and medical sciences; Body fat; Cells; Cells, Cultured; Clinical Medicine; Diabetes. Impaired glucose tolerance; Endocrine pancreas. Apud cells (diseases); Endocrinology and Diabetes; Endocrinopathies; Endokrinologi och diabetes; Etiopathogenesis. Screening. Investigations. Target tissue resistance; Genetic aspects; Glucose; Glucose metabolism; Glucose Transporter Type 4 - genetics; Glucose Transporter Type 4 - metabolism; Homeostasis; Human subjects; Humans; Insulin - pharmacology; Insulin resistance; Klinisk medicin; Medical and Health Sciences; Medical sciences; Medicin och hälsovetenskap; Microscopy, Fluorescence; Original Research; Peptide hormones; Physiological aspects; Protein Transport - drug effects; China; Endocrinopathy; Human; Pancreatic hormone; Insulin; Diabetes mellitus

Systemic glucose homeostasis is profoundly influenced by adipose cell function. Here we investigated GLUT4 dynamics in living adipose cells from human subjects with varying BMI and insulin sensitivity index (Si) values. Cells were transfected with hemagglutinin (HA)-GLUT4-green fluorescent protein (...