Formyl peptide derived lipopeptides disclose differences between the receptors in mouse and men and call the pepducin concept in question

• Published in: PloS one Vol. 12; no. 9; p. e0185132
• Main Authors: Winther, Malene, Holdfeldt, André, Sundqvist, Martina, Rajabkhani, Zahra, Gabl, Michael, Bylund, Johan, Dahlgren, Claes, Forsman, Huamei
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 21.09.2017; Public Library of Science (PLoS)
• Subjects: activation; Amino acids; Animals; Biology and Life Sciences; Cell receptors; Formyl peptide receptors; fpr2; G protein-coupled receptors; Genetic aspects; human neutrophils; Humans; Immunology; Inflammation; Intracellular; Leukocytes (neutrophilic); Leukocytes - metabolism; ligand; Ligands; Lipopeptides; Lipopeptides - administration & dosage; Lipopeptides - metabolism; Medicine; Medicine and Health Sciences; Mice; Mice, Inbred C57BL; Mice, Knockout; Models, Molecular; modulation; NADPH Oxidases - metabolism; nadph-oxidase; Neutrophils; Pattern recognition; Peptides; phagocytes; Physical Sciences; Physiological aspects; Properties; Protein Binding; protein-coupled receptors; Proteins; Receptors; Receptors, Formyl Peptide - genetics; Receptors, Formyl Peptide - metabolism; Receptors, Lipoxin - metabolism; Research and Analysis Methods; respiratory burst; Reumatologi och inflammation; Rheumatology; Rheumatology and Autoimmunity; Science & Technology - Other Topics; Selectivity; Species Specificity; Sweden

A pepducin is a lipopeptide containing a peptide sequence that is identical to one of the intracellular domains of the G-protein coupled receptor (GPCR) assumed to be the target. Neutrophils express two closely related formyl peptide receptors belonging to the family of GPCRs; FPR1 and FPR2 in human...