Development of Real-Time PCR Methods for the Detection of Bacterial Meningitis Pathogens without DNA Extraction - Vuong, Jeni; Collard, Jean-Marc; Whaley, Melissa J.; Bassira, Issaka; Seidou, Issaka; Diarra, Seydou; Ouédraogo, Rasmata T.; Kambiré, Dinanibè; Taylor, Thomas H.; Sacchi, Claudio; Mayer, Leonard W.; Wang, Xin (PloS one, 2016)
Keywords: Analysis; Bacteria; Bacterial meningitis; Bacteriology; Biology and Life Sciences; Cerebrospinal fluid; Contamination; Deoxyribonucleic acid; Diagnosis; Diagnostic systems; Disease control; Disease prevention; DNA; DNA polymerase; DNA, Bacterial; DNA, Bacterial - isolation & purification; Epidemiology; Extraction; Genetic aspects; Haemophilus influenzae; Health risks; Health surveillance; Human health and pathology; Humans; Infectious diseases; Laboratories; Lead; Life Sciences; Limit of Detection; Medicine and Health Sciences; Meningitis; Meningitis, Bacterial; Meningitis, Bacterial - cerebrospinal fluid; Meningitis, Bacterial - diagnosis; Methods; Microbiology and Parasitology; Neisseria meningitidis; Pathogens; Polymerase chain reaction; Public health; Real time; Real-Time Polymerase Chain Reaction; Real-Time Polymerase Chain Reaction - methods; Research and Analysis Methods; Risk reduction; Santé publique et épidémiologie; Sensitivity; Sensitivity and Specificity; Streptococcus infections; Streptococcus pneumoniae; Vaccines; Brazil; Atlanta Georgia; United States--US