Isoforms of U1-70k control subunit dynamics in the human spliceosomal U1 snRNP

• Published in: PloS one Vol. 4; no. 9; p. e7202
• Main Authors: Hernández, Helena, Makarova, Olga V, Makarov, Evgeny M, Morgner, Nina, Muto, Yutaka, Krummel, Daniel Pomeranz, Robinson, Carol V
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 28.09.2009; Public Library of Science (PLoS)
• Subjects: Biochemistry; Biochemistry/Experimental Biophysical Methods; Biochemistry/Macromolecular Assemblies and Machines; Biochemistry/RNA Structure; Catalysis; Dynamic stability; Dynamics; Exons; HeLa Cells; Humans; Isoforms; Laboratories; Mass spectrometry; Mass Spectrometry - methods; Mass spectroscopy; Molecular biology; Molecular Conformation; Phosphorylation; Post-translation; Post-translational modifications; Protein Isoforms; Protein Processing, Post-Translational; Protein structure; Protein Structure, Quaternary; Proteins; Proteomics; Proteomics - methods; Quaternary; Quaternary structure; Recombinant Proteins - chemistry; Ribonucleic acid; Ribonucleoprotein, U1 Small Nuclear - chemistry; Ribonucleoprotein, U1 Small Nuclear - metabolism; Ribonucleoproteins (small nuclear); RNA; RNA - metabolism; RNA, Small Nuclear - metabolism; Scientific imaging; Spectrometry, Mass, Electrospray Ionization - methods; Spliceosomes - metabolism; Trends

Most human protein-encoding genes contain multiple exons that are spliced together, frequently in alternative arrangements, by the spliceosome. It is established that U1 snRNP is an essential component of the spliceosome, in human consisting of RNA and ten proteins, several of which are post-transla...