Cell–cell fusion induced by reovirus FAST proteins enhances replication and pathogenicity of non-enveloped dsRNA viruses

• Published in: PLoS pathogens Vol. 15; no. 4; p. e1007675
• Main Authors: Kanai, Yuta, Kawagishi, Takahiro, Sakai, Yusuke, Nouda, Ryotaro, Shimojima, Masayuki, Saijo, Masayuki, Matsuura, Yoshiharu, Kobayashi, Takeshi
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 25.04.2019; Public Library of Science (PLoS)
• Subjects: Animals; Arthritis; Biology and Life Sciences; Cell Fusion; Double-stranded RNA; Encephalitis; FAST protein; Funding; Fusion protein; Fusion proteins; Glycoproteins; Homology; Infection; Infections; Infectious diseases; Male; Medicine and Health Sciences; Mice; Mice, Inbred C3H; Mutation; Pathogenicity; Pathogens; Physiological aspects; Plasmids; Pneumonia; Proteins; Reoviridae - genetics; Reoviridae - pathogenicity; Reoviridae Infections - genetics; Reoviridae Infections - metabolism; Reoviridae Infections - virology; Replication; Research and Analysis Methods; Risk factors; Rotavirus infections; Veterinary medicine; Viral Fusion Proteins - genetics; Viral Fusion Proteins - metabolism; Virology; Virulence; Virus Replication; Viruses; Japan

Fusogenic reoviruses encode fusion-associated small transmembrane (FAST) protein, which induces cell-cell fusion. FAST protein is the only known fusogenic protein in non-enveloped viruses, and its role in virus replication is not yet known. We generated replication-competent, FAST protein-deficient...