The absence of N-acetylglucosamine in wall teichoic acids of Listeria monocytogenes modifies biofilm architecture and tolerance to rinsing and cleaning procedures

• Published in: PloS one Vol. 13; no. 1; p. e0190879
• Main Authors: Brauge, Thomas, Faille, Christine, Sadovskaya, Irina, Charbit, Alain, Benezech, Thierry, Shen, Yang, Loessner, Martin J, Bautista, Jean Romain, Midelet-Bourdin, Graziella
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 10.01.2018; Public Library of Science (PLoS)
• Subjects: Acetylglucosamine - metabolism; Acids; Adhesion; Architecture; Bacteria; Bacterial Adhesion - genetics; Bacterial Adhesion - physiology; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biofilms; Biology and Life Sciences; Carbohydrates; Cell Wall - genetics; Cell Wall - metabolism; Cell Wall - ultrastructure; Cleaning; Clonal deletion; Colonies; Epidemics; Extracellular matrix; Food products; Food safety; Gene deletion; Gene mutation; Genes; Genetic aspects; Hydrophobic and Hydrophilic Interactions; Laboratories; Life Sciences; Listeria; Listeria monocytogenes; Listeria monocytogenes - genetics; Listeria monocytogenes - physiology; Listeria monocytogenes - ultrastructure; Medicine and Health Sciences; Microbiology and Parasitology; Microscopy, Electron, Transmission; Mutagenesis; Mutants; Mutation; N-Acetylglucosamine; Nutrition; Phenotype; Physical Sciences; Point mutation; Seafood; Shear stress; Splicing; Stainless Steel; Stainless steels; Stress, Mechanical; Structural steels; Teichoic acids; Teichoic Acids - metabolism; Water; Water flow; Biofilm; Force d'interaction; Fraction saccharidique; Listeria; Acide téichoïque

The wall teichoic acid (WTA) is the major carbohydrate found within the extracellular matrix of the Listeria monocytogenes biofilm. We first addressed the frequency of spontaneous mutations in two genes (lmo2549 and lmo2550) responsible for the GlcNAcylation in 93 serotype 1/2a strains that were mai...